期刊
MICROCHIMICA ACTA
卷 175, 期 1-2, 页码 79-86出版社
SPRINGER WIEN
DOI: 10.1007/s00604-011-0645-9
关键词
Glucose oxidase; Polypyrrole; Enzymatic synthesis; Self-assembled monolayer; Gold surface
资金
- European Union [SF-PD-2009-08-17-0151]
We describe a new method for the visualization of the activity of red-ox proteins on a gold interface. Glucose oxidase was selected as a model system. Surfaces were modified by adhesion of glucose oxidase on (a) electrochemically cleaned gold; (b) gold films modified with gold nanoparticles, (c) a gold surface modified with self-assembled monolayer, and (d) covalent immobilization of protein on the gold surface modified with a self-assembled monolayer. The simple optical method for the visualization of enzyme on the surfaces is based on the enzymatic formation of polypyrrole. The activity of the enzyme was quantified via enzymatic formation of polypyrrole, which was detected and investigated by quartz microbalance and amperometric techniques. The experimental data suggest that the enzymatic formation of the polymer may serve as a method to indicate the adhesion of active redox enzyme on such surfaces.
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