Chitosan matrices modified with carbon nanotubes for use in mediated microbial biosensing

We describe a microbial sensor based on Pseudomonas fluorescens cells that was prepared by modifying graphite electrodes with chitosan and carbon nanotubes. Chronoamperometry was performed at +0.3 V in the presence of hexacyanoferrate as a mediator and revealed a good response to glucose which is linear in the 1.0 to 5.0 mM concentration range. Linearity was defined by the equation of y = 102.120x-13.279 (R (2) = 0.998) (y shows current density as nA.cm(-2) and x shows glucose concentration in mM). The effect of the CNTs on the response was compared to that of electrodes made without CNTs.