期刊
MICROCHIMICA ACTA
卷 172, 期 3-4, 页码 247-260出版社
SPRINGER WIEN
DOI: 10.1007/s00604-010-0519-6
关键词
Metallointercalator; Biosensor; Signal amplification; DNA binding; DNA damage
资金
- National Science Foundation [NSF-0901303]
- WV EPSCoR
- National Natural Science Foundation of China [20890112, 20825519, 20921063]
- Div Of Electrical, Commun & Cyber Sys
- Directorate For Engineering [0901303] Funding Source: National Science Foundation
Strategies for electrochemical sensing of DNA can be classified into label-free and label-based approaches, categories of which include enzyme-, nanomaterial- and redox labels that are attached to DNA either by covalent or non-covalent means. Metallointercalators represent one group of small molecule redox labels that non-covalently enter the groove of a DNA. The metallointercalator plays a dual-role in acting as a structure indicator (for hybridization) and a signal generator. Labeling is not needed, and electrochemical measurements can be carried out in a label-free solution of an electrolyte. However, such metallointercalators lack the option of catalytic signal generation as in the case of enzyme-and nanomaterial-based labels. Therefore, signal amplification becomes crucial. We first survey here recent progress in this area. A signal-amplifying system is presented that relies on the electroatalytic oxidation of a metallointercalator ruthenium(II) bipyridine/phenoxazine complex in the presence of electron donor species such as oxalate, DNA bases, or tripropylamine. Recent work on such DNA sensors is discussed. Results suggest that such metallointercalator-based DNA sensors represent a viable platform for developing high-throughput and automated PCR/lab-on-a-chip devices as well as visualized multifunctional DNA sensors.
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