期刊
MICROCHIMICA ACTA
卷 171, 期 3-4, 页码 385-392出版社
SPRINGER WIEN
DOI: 10.1007/s00604-010-0444-8
关键词
Cu-Mg-Al layered double hydroxide; Nafion; Cytochrome c; Nitrite biosensor; Direct electrochemistry; Determination
资金
- National Natural Science Foundation of China [20775044]
- Natural Science Foundation of Shandong province, China [Y2006B20]
An amperometric biosensor for nitrite was prepared by immobilizing cytochrome c (Cyt c) on a gold electrode that was modified with Nafion and a Cu-Mg-Al layered double hydroxide (Cu-LDH). The Cu-LDH was characterized by Fourier transfom infrared spectroscopy and powder X-ray diffraction. The UV-visible spectrum suggests that Cyt c retains its native conformation in the modified film. The direct electrochemical investigation indicated that the composite film represents a good platform for the immobilization of Cyt c as well as an excellent promoter for the electron transfer between Cyt c and the gold electrode. Moreover, the biosensor showed a remarkable bioelectrocatalytic activity for the oxidation of nitrite with a linear range from 0.75 to 123 mu M. The detection limit is 2x10(-7) M (S/N=3). The biosensor was successfully applied to the determination of nitrite in food samples.
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