Article
Microbiology
Pierre-Etienne Marcoux, Antony T. Vincent, Marie-Ange Massicotte, Valerie E. Paquet, Emilie J. Doucet, Nava Hosseini, Melanie V. Trudel, Gabriel Byatt, Mathilde Laurent, Michel Frenette, Steve J. Charette
Summary: European strains of Aeromonas salmonicida subsp. salmonicida are more prone to losing the TTSS locus compared to Canadian strains. A cluster of genes encoding putative proteins with DNA binding capacity and phage proteins was found to be almost perfectly correlated with the susceptibility of different strains to lose the locus. This discovery opens up new opportunities for studying the thermosensitivity of pAsa5.
Article
Microbiology
Hongyan Cai, Jiaying Yu, Ying Qiao, Ying Ma, Jiang Zheng, Mao Lin, Qingpi Yan, Lixing Huang
Summary: Aeromonas salmonicida, a psychrophilic bacterial pathogen, is a major threat to salmon farming, causing significant economic losses. This study found that Hcp is an important virulence gene in A. salmonicida SRW-OG1.
Article
Multidisciplinary Sciences
Gabriel U. Oka, Diorge P. Souza, William Cenens, Bruno Y. Matsuyama, Marcus V. C. Cardoso, Luciana C. Oliveira, Filipe da Silva Lima, Iolanda M. Cuccovia, Cristiane R. Guzzo, Roberto K. Salinas, Chuck S. Farah
Summary: The study identified an interaction between XVIPCD and VirD4, dividing XVIPCD into two regions with distinct functions. The N-terminal region interacts with VirD4, while both the N- and C-terminal regions are required for efficient X-Tfe translocation into target cells. The conformational stability of the N-terminal region is reduced at lower pH, potentially facilitating X-Tfe unfolding and translocation.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Biotechnology & Applied Microbiology
Xiaoye Liang, Tong-Tong Pei, Zeng-Hang Wang, Weiliang Xiong, Li-Li Wu, Ping Xu, Shuangjun Lin, Tao G. Dong
Summary: The Type VI Secretion System (T6SS) is utilized by Gram-negative bacteria for interspecies interaction. This study identified a lysozyme-like effector TseP in Aeromonas dhakensis, showcasing its cell wall-lysing activities and T6SS dependency. Inactivating TseP and two other effectors led to the creation of a detoxified T6SS-active strain for potential protein delivery to various recipient cells.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2021)
Article
Microbiology
Sophie A. Howard, R. Christopher D. Furniss, Dora Bonini, Himani Amin, Patricia Paracuellos, David Zlotkin, Tiago R. D. Costa, Asaf Levy, Despoina A. I. Mavridou, Alain Filloux
Summary: The T6SS is a bacterial nanoscale weapon that delivers toxins into prey and plays a key role in the arms race between organisms. By using Hcp as bait, researchers identified uncharacterized toxins and investigated the role of Hcp in the T6SS mechanism.
Article
Chemistry, Applied
Liangting Shao, Shanshan Chen, Zhenzhen Ning, Xinglian Xu, Huhu Wang
Summary: In this study, a hemagglutinin protease (Hap) belonging to the M4 family metallopeptidase was identified from Aeromonas salmonicida 29 isolate, which is conserved in various dominant spoilage bacteria. Hap demonstrated high spoilage ability on meat, increasing TVB-N, pH values, and the production of volatile organic compounds (VOCs), confirming its determinant role for meat spoilage. These findings will enrich the spoilage theory and provide new insights into the control of food quality and safety.
Article
Multidisciplinary Sciences
Marc-Andre Leblanc, Morgan R. Fink, Thomas T. Perkins, Marcelo C. Sousa
Summary: The study shows that effector proteins from certain bacteria can be injected into host cells through a type III secretion system. These proteins need to be partially unfolded to pass through the narrow channel. Mechanical unfolding of the proteins revealed that they exhibit low force and high mechanical compliance, facilitating efficient secretion.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Microbiology
B. Izquierdo Lafuente, R. Ummels, C. Kuijl, W. Bitter, A. Speer
Summary: CpnT, a potent secreted toxin of Mycobacterium tuberculosis, is exclusively secreted by the ESX-5 system in bacterial culture but relies on intact ESX-1, ESX-4, and ESX-5 systems during infection studies. This highlights the intricate interplay of three different secretion systems in secreting one substrate during infection.
Review
Biochemistry & Molecular Biology
Julia Sanchez-Garrido, David Ruano-Gallego, Jyoti S. Choudhary, Gad Frankel
Summary: Recent studies have shown that T3SS effectors interact with each other in the host, revealing their interdependency and context-dependent essentiality, which is of great significance for understanding the pathogenesis of the pathogens.
TRENDS IN MICROBIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Dongying Liu, Yantao Luo, Xiaofeng Zheng, Xinye Wang, Minxia Chou, Gehong Wei
Summary: NopP, a T3SS effector of Mesorhizobium amorphae CCNWGS0123, is secreted through T3SS-I during early infection stages and its deficiency reduces nitrogenase activity in Robinia pseudoacacia nodules. TRAPPC13 has been identified as a target protein of NopP in R. pseudoacacia roots, showing colocalization on the plasma membrane. The interaction between NopP and TRAPPC13 affects genes associated with cell wall remodeling and plant innate immunity in early infection stages.
MOLECULAR PLANT-MICROBE INTERACTIONS
(2021)
Article
Plant Sciences
Irene Jimenez-Guerrero, Francisco Javier Lopez-Baena, Carlos Medina
Summary: Rhizobia establish mutually beneficial interactions with legume plants through nodulation, where the bacteria fix atmospheric nitrogen. The compatibility of this interaction is determined by bacterial recognition of flavonoids secreted by plants, which trigger the synthesis of Nod factors. Other bacterial signals, such as extracellular polysaccharides and secreted proteins, also contribute to the efficiency of the interaction.
Article
Virology
Nava Hosseini, Valerie E. Paquet, Mahdi Chehreghani, Sylvain Moineau, Steve J. Charette
Summary: Aquaculture is facing antibiotic resistance issues in fighting pathogenic bacteria, with phage therapy being a potential alternative. A specific combination of four phages has shown effectiveness against A. salmonicida subsp. salmonicida, but strains with Prophage 3 exhibit reduced sensitivity. This highlights the impact of prophage content on the efficacy of phage therapy.
Article
Biochemistry & Molecular Biology
Manisha Yadav, Mahalashmi Srinivasan, Nikhil K. Tulsian, Yu Xuan Liu, Qingsong Lin, Ilan Rosenshine, J. Sivaraman
Summary: This study identified the specific interaction between CesT and its key effector, NleH2, and explored the potential role of NleH2 as a kinase for CesT phosphorylation. Effector proteins bind to CesT at the beta-sheet core and dimer interface, while the CsrA regulator interacts predominantly through the C-terminal region. NleH2 was found to remain an active kinase even in complex with CesT and is responsible for autophosphorylation and phosphorylation of CesT at Tyr153.
Article
Multidisciplinary Sciences
Catalina Rodriguez-Puerto, Rupak Chakraborty, Raksha Singh, Perla Rocha-Loyola, Clemencia M. Rojas
Summary: The plant pathogenic bacterium Pseudomonas syringae pv. tomato DC3000 (Pst DC3000) is capable of suppressing plant defense responses and promoting pathogenicity through its type III secretion system and a mitochondrial-localized effector, HopG1. HopG1 triggers necrotic cell death and interacts with a plant immunity-related protein, AtNHR2B, which attenuates its virulence functions.
SCIENTIFIC REPORTS
(2022)
Article
Veterinary Sciences
Jiajia Wang, Lijun Xiu, Ying Qiao, Youyu Zhang
Summary: It was previously believed that psychrophilic Aeromonas salmonicida could not infect mammals and humans due to its inability to grow above 25 degrees C. However, a new study found that a specific strain of mesophilic A. salmonicida isolated from Epinephelus coioides was able to cause furunculosis. The researchers discovered that the Zn2+ uptake related genes znuA, znuB, and znuC might play a role in the virulence regulation of A. salmonicida SRW-OG1. Silencing these genes resulted in restricted growth under Fe2+ starvation and decreased motility, biofilm formation, adhesion, and hemolysis. The expression of znuABC was also found to be affected by different growth conditions and stressors.
FRONTIERS IN VETERINARY SCIENCE
(2023)
Article
Biochemical Research Methods
Andree-Eve Chretien, Isabelle Gagnon-Arsenault, Alexandre K. Dube, Xavier Barbeau, Philippe C. Despres, Claudine Lamothe, Anne-Marie Dion-Cote, Patrick Lague, Christian R. Landry
MOLECULAR & CELLULAR PROTEOMICS
(2018)
Article
Biochemistry & Molecular Biology
Paresh M. Vadhadiya, Marc-Alexandre Jean, Chahrazed Bouzriba, Thomas Tremblay, Patrick Lague, Sebastien Fortin, John Boukouvalas, Denis Giguere
Article
Chemistry, Medicinal
Elsa Forcellini, Sophie Boutin, Carole-Anne Lefebvre, Elnur Elyar Shayhidin, Marie-Chloe Boulanger, Gabrielle Rheaume, Xavier Barbeau, Patrick Lague, Patrick Mathieu, Jean-Francois Paquin
EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY
(2018)
Correction
Biochemical Research Methods
Andree-Eve Chretien, Isabelle Gagnon-Arsenault, Alexandre K. Dube, Xavier Barbeau, Philippe C. Despres, Claudine Lamothe, Anne-Marie Dion-Cote, Patrick Lague, Christian R. Landry
MOLECULAR & CELLULAR PROTEOMICS
(2018)
Article
Biochemistry & Molecular Biology
Maxime Lesperance, Xavier Barbeau, Jenny Roy, Rene Maltais, Patrick Lague, Donald Poirier
Article
Biochemistry & Molecular Biology
Claudia Carpentier, Xavier Barbeau, Jabrane Azelmat, Katy Vaillancourt, Daniel Grenier, Patrick Lague, Normand Voyer
BIOORGANIC & MEDICINAL CHEMISTRY
(2018)
Article
Chemistry, Multidisciplinary
Pierre-Alexandre Paquet-Cote, Matthieu Fillion, Marie-Eve Provencher, Francois Otis, Justine Dionne, Sebastien Cardinal, Barbara Collignon, Jochen Buerck, Patrick Lague, Anne S. Ulrich, Michele Auger, Normand Voyer
SUPRAMOLECULAR CHEMISTRY
(2019)
Article
Pharmacology & Pharmacy
Yacine Abed, Clement Fage, Liva Checkmahomed, Gabriel Begin, Julie Carbonneau, Patrick Lague, Guy Boivin
ANTIVIRAL RESEARCH
(2019)
Article
Biochemistry & Molecular Biology
Van Dung Pham, Tuan Anh To, Cynthia Gagne-Thivierge, Manon Couture, Patrick Lague, Deqiang Yao, Marie-Eve Picard, Louis-Andre Lortie, Sabrina A. Attere, Xiaojun Zhu, Roger C. Levesque, Steve J. Charette, Rong Shi
JOURNAL OF BIOLOGICAL CHEMISTRY
(2020)
Article
Engineering, Chemical
Dariush Azizi, Faical Larachi, Alain Garnier, Patrick Lague, Benoit Levasseur
Summary: Through density functional theory (DFT) simulations, this study examined the interaction between 20 natural amino acids and several mineral surfaces, finding that the adsorption affinities of amino acids vary at different pH values, with pyrite showing the highest affinity. The results of the research help in selecting the most suitable amino acid collectors for improved separation of sulphidic minerals in flotation processes.
CANADIAN JOURNAL OF CHEMICAL ENGINEERING
(2021)
Article
Biochemistry & Molecular Biology
N. T. Hang Pham, Myriam Letourneau, Marlene Fortier, Gabriel Begin, M. Sameer Al-Abdul-Wahid, Fabrizio Pucci, Benjamin Folch, Marianne Rooman, David Chatenet, Yves St-Pierre, Patrick Lague, Charles Calmettes, Nicolas Doucet
Summary: This study successfully modulated the immunosuppressive protein GAL-7's activity through protein engineering and mutational techniques, demonstrating that perturbing the protein-protein interface can affect its biological function, highlighting new avenues for the design of galectin-specific modulators.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2021)
Article
Infectious Diseases
Samuel Cashman-Kadri, Patrick Lague, Ismail Fliss, Lucie Beaulieu
Summary: This study investigated the structure-activity relationships and mode of action of synthesized GAPDH-related antimicrobial peptides. It was found that increasing the net positive charge and hydrophobicity improved the antimicrobial and antifungal activities of the peptides. The peptides were shown to cause membrane permeabilization and target intracellular components, indicating a mixed mode of action.
Article
Nanoscience & Nanotechnology
Abolfazl Alizadeh Sahraei, Barbara Mejia Bohorquez, Denise Tremblay, Sylvain Moineau, Alain Garnier, Faical Larachi, Patrick Lague
Summary: This study aimed to evaluate the interactions of peptides with quartz as potential flotation collectors, in order to find biofriendly alternatives to biohazardous chemical reagents in mining practices. The results showed that the flexibility of peptide chains directly affected their adsorption behavior. Overall, this study is of great significance for the rational design of peptide sequences for mineral processing.
ACS APPLIED MATERIALS & INTERFACES
(2023)
Article
Infectious Diseases
Samuel Cashman-Kadri, Patrick Laguee, Ismail Fliss, Lucie Beaulieu
Summary: This study analyzed the antimicrobial activity of SJGAP and its analogs under different physicochemical conditions. The presence of divalent cations inhibited their antibacterial activity, while monovalent cations had a lesser impact and could even promote the activity of the native SJGAP. The peptides showed higher activity at acidic pH values, but their stability varied after a heat treatment. Additionally, the peptides exhibited low hemolytic activity, especially degrading rapidly in the stomach compartment of the digestion model.
Letter
Immunology
Yacine Abed, Clement Fage, Patrick Lague, Julie Carbonneau, Jesse Papenburg, Donald C. Vinh, Guy Boivin
EMERGING INFECTIOUS DISEASES
(2019)