期刊
MICROBIOLOGY-SGM
卷 158, 期 -, 页码 1482-1492出版社
SOC GENERAL MICROBIOLOGY
DOI: 10.1099/mic.0.057745-0
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资金
- Ministry of Education, Culture, Sports, Science and Technology of Japan [17076016, 8310133, 21241047]
- Micro-Nano Technology Research Center of Hosei University
- Grants-in-Aid for Scientific Research [17076016] Funding Source: KAKEN
The BasS-BasR two-component system is known as an iron- and zinc-sensing transcription regulator in Escherichia coli, but so far only a few genes have been identified to be under the direct control of phosphorylated BasR. Using Genomic SELEX (systematic evolution of ligands by exponential enrichment) screening, we have identified a total of at least 38 binding sites of phosphorylated BasR on the E. coli genome, and based on the BasR-binding sites, have predicted more than 20 novel targets of regulation. By DNase I footprint analysis for high-affinity BasR-binding sites, a direct repeat of a TTAAnnTT sequence was identified as the BasR box. Transcription regulation in vivo of the target genes was confirmed after Northern blot analysis of target gene mRNAs from both wild-type E. coli and an otherwise isogenic basR deletion mutant. The BasR regulon can be classified into three groups of genes: group 1 includes the genes for the formation and modification of membrane structure; group 2 includes genes for modulation of membrane functions; and group 3 includes genes for stress-response cell functions, including csgD, the master regulator of biofilm formation.
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