Article
Ecology
Aliona S. Rosca, Joana Castro, Angela Franca, Mario Vaneechoutte, Nuno Cerca
Summary: Bacterial vaginosis (BV) is a common bacterial vaginal infection with an unknown etiology, characterized by structured polymicrobial biofilms primarily formed by Gardnerella spp. and other anaerobic species. The study compared triple-species biofilms using pre-conditioned and competitive models, finding more significant synergistic growth interactions in the competitive model. Despite model differences, G. vaginalis consistently dominated the biofilm composition, emphasizing its relevance in BV.
Article
Biotechnology & Applied Microbiology
Mor Tsamir-Rimon, Elhanan Borenstein
Summary: This study successfully characterizes the progression from healthy Lactobacillus-dominant populations to Bacterial vaginosis (BV) and accurately quantifies the health condition of samples along this route by analyzing 8026 vaginal samples and using a manifold-detection framework.
NPJ BIOFILMS AND MICROBIOMES
(2023)
Article
Microbiology
Xiaolu Ma, Xiaoxi Wang, Shengna Ye, Jinnan Liu, Hong Yuan, Nan Wang
Summary: This study analyzed the pathogenic factors and drug sensitivity associated with clinical treatment of bacterial vaginosis (BV) in Northeast China. The results showed that Gardnerella vaginalis is more resistant to metronidazole than clindamycin, and neither antibiotic is able to effectively eradicate vaginal biofilms.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Microbiology
Hanyu Qin, Yun Liu, Zhengyuan Zhai, Bingbing Xiao
Summary: This study found that different subgroups of Gardnerella exhibit differences in biofilm formation ability and antibiotic resistance. Strains with strong biofilm ability contain more genes involved in carbohydrate synthesis and metabolism. These findings contribute to the development of novel treatments for bacterial vaginosis.
Article
Biotechnology & Applied Microbiology
Tongyang Deng, Anquan Shang, Ying Zheng, Lianzhen Zhang, Hong Sun, Wei Wang
Summary: The aim of this study was to explore a new marker for the quantitative detection of bacterial vaginosis (BV) with high sensitivity and specificity. By comparing the differences in bacterial abundance between BV, healthy, and BV-intermediate groups using 16S rRNA-sequencing, candidate markers were identified. Real-time reverse transcription-quantitative polymerase chain reaction (RT-qPCR) was then used to quantitatively detect these candidate markers and evaluate their sensitivity and specificity. Four new indicators for BV detection were discovered, with log L. crispatus/G. vaginalis (L/G) < 0 being the best indicator. A molecular diagnostic system independent of the Nugent Score could have an important impact on the clinical management of BV.
Article
Microbiology
Qiuxiang Zhang, Qiuhan Cheng, Shumao Cui, Jianxin Zhao, Wei Chen, Hao Zhang
Summary: This study evaluated the inhibitory effect of Lactobacillus gasseri CCFM1201 on Gardnerella vaginalis, the core pathogen of bacterial vaginosis. In vitro and in vivo experiments showed that L. gasseri CCFM1201 can inhibit the formation of G. vaginalis biofilm and its adhesion capacity. Furthermore, L. gasseri CCFM1201 reduced G. vaginalis in mice, modulated the inflammatory response, and improved histopathological damage to vaginal tissue.
ARCHIVES OF MICROBIOLOGY
(2022)
Article
Microbiology
Hyemin Kim, YongGyeong Kim, Chang-Ho Kang
Summary: This study evaluated the effects of three lactic acid bacteria on bacterial vaginosis in a mice model, showing that they inhibited the growth of Gardnerella vaginalis, reduced the expression of inflammatory cytokines, and decreased exfoliation of vaginal epithelial cells. These lactic acid bacteria also demonstrated good tolerance, probiotic effects, and potential as food ingredients for ameliorating BV in women.
Review
Immunology
Omid Gholizadeh, Zahra Yekanipour, Setareh Kivi, Farnoosh Farahbod, Mohadeseh Aqayinejad, Yalda Tasoujizade, Saman Yasamineh, Parya Amini, Sama Akbarzadeh, Mehrnaz Hajiesmaeili, Vahdat Poortahmasebi
Summary: The human papillomavirus (HPV) is a common sexually transmitted infection, typically acquired after the first sexual activity. Most HPV infections are asymptomatic and temporary. Recurrent infections with high-risk HPV strains can lead to various cancers later in life. HPV infections are also significantly associated with reproductive function abnormalities, including male infertility and increased risk of miscarriage and premature labor. Gardnerella vaginalis (GV), an anaerobic bacterium, can contribute to bacterial vaginosis (BV) and reduced fertility in women. More research is needed to understand the exact mechanisms of how GV and HPV cause infertility.
MICROBIAL PATHOGENESIS
(2023)
Review
Immunology
Xiaodi Chen, Yune Lu, Tao Chen, Rongguo Li
Summary: The vaginal microbiome is crucial for women's health, with bacterial vaginosis (BV) associated with various adverse gynecologic and obstetric outcomes. Research on diagnosis and treatment strategies for BV is making progress.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2021)
Article
Veterinary Sciences
Jinok Kwak, Sriniwas Pandey, Jinho Cho, Minho Song, Eun Sol Kim, Hyunok Doo, Gi Beom Keum, Sumin Ryu, Yejin Choi, Juyoun Kang, Sheena Kim, Jeongyun Kim, Hyeun Bum Kim
Summary: This study developed a mouse model for human BV induced by G. vaginalis, and demonstrated comparable clinical attributes to patients with BV.
FRONTIERS IN VETERINARY SCIENCE
(2023)
Article
Infectious Diseases
Agnieszka Latka, Leen Van Simaey, Marijke Reynders, Piet Cools, Tess Rogier, Barbara Lebbe, Lorenzo Corsini, Christine Landlinger, Mario Vaneechoutte
Summary: The quantification of living cells in biofilm or after eradication treatments is problematic. This study assessed the performance of pre-treatment with propidium monoazide (PMAxx) to prevent qPCR-based amplification of DNA from killed cells. The optimized PMAxx treatment condition showed a significant reduction of Gardnerella cells, validating its use in vaginal samples from women with bacterial vaginosis.
Article
Microbiology
Soo-Im Choi, GaYeong Won, YongGyeong Kim, Chang-Ho Kang, Gun-Hee Kim
Summary: This study investigated the effects of a mixture of five lactobacilli strains on bacterial vaginitis (BV) in HeLa cells and GV-infected BV mice. The findings suggest that this mixture can inhibit the growth of GV, reduce inflammation, and suppress the exfoliation of vaginal epithelial cells.
Article
Biotechnology & Applied Microbiology
Yu-Ping Huang, Jie-Yan Shi, Si-Chen Luo, Shao-Yan Xu, Jia-Dong Zhang, Istvan Molnar, Qiong-Qiong Yang, Bo-Bo Zhang
Summary: This study found that acetic acid and lactic acid in the cell-free supernatant of L. rhamnosus inhibit the growth of G. vaginalis by altering the morphology of the bacteria and affecting their ATP synthesis. The results provide valuable information for the use of L. rhamnosus in the treatment of bacterial vaginosis.
PROBIOTICS AND ANTIMICROBIAL PROTEINS
(2023)
Article
Immunology
Chia-Wen Liu, Bor-Chyuan Su, Jyh-Yih Chen
Summary: This study demonstrated that TP4 can attenuate the virulence of G. vaginalis by inducing resolving M2c and tissue repair M2a macrophage polarizations. TP4 switches M1 macrophages toward a resolving M2c phenotype via the MAPK/ERK pathway and IL-10-STAT3 signaling. Additionally, TP4-treated VK2 cells induce tissue repair M2a macrophages by upregulating TSG-6 and subsequently activating STAT6.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Infectious Diseases
Joana Castro, Lucia G. V. Sousa, Angela Franca, Lenka Podpera Tisakova, Lorenzo Corsini, Nuno Cerca
Summary: Bacterial vaginosis (BV) is a common vaginal infection in reproductive-age women. This study investigated the role of an investigational engineered phage endolysin in eradicating biofilms associated with BV.
Article
Obstetrics & Gynecology
Matthew Josiah Allen-Daniels, Myrna G. Serrano, Lindsey P. Pflugner, Jennifer M. Fettweis, Melissa A. Prestosa, Vishal N. Koparde, J. Paul Brooks, Jerome F. Strauss, Roberto Romero, Tinnakorn Chaiworapongsa, David A. Eschenbach, Gregory A. Buck, Kimberly K. Jefferson
AMERICAN JOURNAL OF OBSTETRICS AND GYNECOLOGY
(2015)
Article
Microbiology
J. Paul Brooks, David J. Edwards, Michael D. Harwich, Maria C. Rivera, Jennifer M. Fettweis, Myrna G. Serrano, Robert A. Reris, Nihar U. Sheth, Bernice Huang, Philippe Girerd, Jerome F. Strauss, Kimberly K. Jefferson, Gregory A. Buck
Article
Multidisciplinary Sciences
Joana Castro, Patricia Alves, Carmen Sousa, Tatiana Cereija, Angela Franca, Kimberly K. Jefferson, Nuno Cerca
SCIENTIFIC REPORTS
(2015)
Article
Microbiology
Abdallah A. Abdelmaksoud, Vishal N. Koparde, Nihar U. Sheth, Myrna G. Serrano, Abigail L. Glascock, Jennifer M. Fettweis, Jerome F. Strauss, Gregory A. Buck, Kimberly K. Jefferson
Article
Multidisciplinary Sciences
Nabil M. Abraham, Lei Liu, Brandon Lyon Jutras, Akhilesh K. Yadav, Sukanya Narasimhan, Vissagan Gopalakrishnan, Juliana M. Ansari, Kimberly K. Jefferson, Felipe Cava, Christine Jacobs-Wagner, Erol Fikrig
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2017)
Review
Microbiology
Lita M. Proctor
CELL HOST & MICROBE
(2014)
Article
Medical Laboratory Technology
Bernice Huang, Jennifer M. Fettweis, J. Paul Brooks, Kimberly K. Jefferson, Gregory A. Buck
CLINICS IN LABORATORY MEDICINE
(2014)
Article
Microbiology
Jennifer M. Fettweis, J. Paul Brooks, Myrna G. Serrano, Nihar U. Sheth, Philippe H. Girerd, David J. Edwards, Jerome F. Strauss, Kimberly K. Jefferson, Gregory A. Buck
Article
Multidisciplinary Sciences
Jennifer M. Fettweis, Myrna G. Serrano, Bernice Huang, J. Paul Brooks, Abigail L. Glascock, Nihar U. Sheth, Jerome F. Strauss, Kimberly K. Jefferson, Gregory A. Buck
Article
Multidisciplinary Sciences
Naren Gajenthra Kumar, Daniel Contaifer, Paul R. S. Baker, Kim Ekroos, Kimberly K. Jefferson, Dayanjan S. Wijesinghe
Article
Obstetrics & Gynecology
Kimberly K. Jefferson, Hardik Parikh, Erin M. Garcia, David J. Edwards, Myrna G. Serrano, Martin Hewison, Judith R. Shary, Anna M. Powell, Bruce W. Hollis, Jennifer M. Fettweis, Jerome F. Strauss, Gregory A. Buck, Carol L. Wagner
JOURNAL OF PERINATOLOGY
(2019)
Article
Multidisciplinary Sciences
Naren Gajenthra Kumar, Daniel Contaifer, Dayanjan S. Wijesinghe, Kimberly K. Jefferson
Summary: Bacterial lipases, including the newly discovered SAL3 from Staphylococcus aureus, play crucial roles during infection by specifically targeting short chain fatty acids and binding negatively charged lipids. A catalytic triad mutation can abolish the lipase activity of SAL3 while maintaining its binding ability to host lipid substrates.
Article
Genetics & Heredity
Abigail L. Glascock, Nicole R. Jimenez, Sam Boundy, Vishal N. Koparde, J. Paul Brooks, David J. Edwards, Jerome F. Strauss, Kimberly K. Jefferson, Myrna G. Serrano, Gregory A. Buck, Jennifer M. Fettweis
Summary: The study reveals that there are differences in genomic structure and metabolic potential between two vaginal bacterial taxa grouped within the genus Megasphaera, which have distinct impacts on the vaginal environment. MP1 is strongly associated with bacterial vaginosis, while MP2 is positively linked with trichomoniasis. Additionally, MP1 remains present and transcriptionally active in pregnancy, while MP2 is largely absent in pregnant women.
MICROBIAL GENOMICS
(2021)
Article
Multidisciplinary Sciences
Cathyrn R. O'Brien, Jacob Raskin, Ivypel Amankwa Asare, Christine Wei, Joy T. Ma, Zion K. McCoy, Kimberly Jefferson
Summary: The association between Sneathia vaginalis and preterm birth is being explored. The bacteria produces a large exotoxin called cytopathogenic toxin A (CptA), which forms pores in human epithelial cells and red blood cells. A recombinant protein containing the structured amino-terminal portion of CptA was found to be sufficient to permeabilize cells, while the repeat region of the protein bound to cells but did not cause permeability or lysis of red blood cells. This analysis sheds light on the mechanism of action of CptA, the only virulence factor of S. vaginalis studied to date.
Article
Obstetrics & Gynecology
J. Paul Brooks, David J. Edwards, Diana L. Blithe, Jennifer M. Fettweis, Myrna G. Serrano, Nihar U. Sheth, Jerome F. Strauss, Gregory A. Buck, Kimberly K. Jefferson