Article
Microbiology
Jaclyn A. Cusumano, Kathryn E. Daffinee, Paul Ugalde-Silva, Wolfgang Peti, Michel Arthur, Charlene Desbonnet, Louis B. Rice, Kerry L. LaPlante, Monica Garcia-Solache
Summary: Ampicillin-ceftriaxone is an effective treatment for Enterococcus faecalis endocarditis. This study characterized the penicillin-binding protein (PBP) profiles of different E. faecalis strains and tested for synergy to optimize beta-lactam treatment options for E. faecalis infections. Increased expression of PBP4 and PBP2B was observed in a strain with elevated MICs, but PBP2B did not significantly contribute to the elevated beta-lactam MICs. The combination of meropenem plus ceftriaxone or ertapenem plus ceftriaxone showed the most consistent synergistic activity.
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2023)
Article
Microbiology
Wenlin Yang, Qiu Xu, Qin Yang, Lingli Wang, Siguo Liu, Yao Zhu, Wanjiang Zhang
Summary: The occurrence and dissemination of linezolid-resistant Gram-positive bacteria among food-producing animals poses severe threats to public health. In this study, the optrA resistance gene was found in a multi-drug resistant strain of E.faecalis isolated from a goat in China. The strain belonged to the high-risk clone ST16 and carried the optrA gene on a novel plasmid. Active surveillance of optrA-positive E.faecalis high-risk clones in food-producing animals is urgently warranted.
VETERINARY MICROBIOLOGY
(2022)
Article
Microbiology
Renata Kwit, Magdalena Zajac, Aleksandra Smialowska-Weglinska, Magdalena Skarzynska, Arkadiusz Bomba, Anna Lalak, Ewelina Skrzypiec, Dominika Wojdat, Weronika Koza, Emilia Mikos-Wojewoda, Paulina Pasim, Milena Skora, Marcin Polak, Jaroslaw Wiacek, Dariusz Wasyl
Summary: This study aimed to assess the prevalence of Enterococcus spp. in wild birds in Poland, determine their antimicrobial susceptibility, and conduct Whole Genome Sequencing (WGS) analysis. The results showed that out of 138 samples from various species of free-living birds, 66.7% tested positive for Enterococcus. Fourteen species were detected, with Enterococcus faecalis being the most common. Resistance to antimicrobial agents was observed in some E. faecalis and E. faecium strains, along with the presence of multidrug resistance phenotype in one E. faecium strain. WGS analysis also revealed variations in virulence gene diversity between E. faecalis and E. faecium strains, as well as the presence of plasmid replicons in both strains.
Review
Chemistry, Medicinal
Islam A. A. Ali, Prasanna Neelakantan
Summary: This review discusses the antimicrobial tolerance attributes of Enterococcus faecalis biofilms, explores the potential of using phytochemicals to combat microbial pathogens, and reports the antibiofilm activity and mechanisms of action of these compounds against E. faecalis.
PHYTOTHERAPY RESEARCH
(2022)
Article
Microbiology
Stephen Wandro, Pooja Ghatbale, Hedieh Attai, Clark Hendrickson, Cyril Samillano, Joy Suh, Sage J. B. Dunham, David T. Pride, Katrine Whiteson
Summary: This study isolated and developed a collection of Enterococcus phages that can infect both E faecalis and E. faecium. It was found that combining phages into cocktails often prevented the growth of phage-resistant mutants. Genomic characterization showed consistent mutations in exopolysaccharide synthesis genes when resistant mutants emerged. These findings inform efforts to improve efficacy against Enterococcus isolates and reduce the emergence of resistance.
Article
Microbiology
Diane Soussan, Marine Salze, Pierre Ledormand, Nicolas Sauvageot, Amine Boukerb, Olivier Lesouhaitier, Gwennaele Fichant, Alain Rince, Yves Quentin, Cecile Muller
Summary: In this study, the role of the NagY antiterminator in regulating the nagY-nagE operon and HylA virulence factor expression in the presence of N-acetylglucosamine was investigated. It was found that NagY regulates target gene expression through the interaction of an RNA antiterminator sequence overlapping a rho-independent terminator. This study provides new insights into the mechanism of host sensing in Enterococcus faecalis.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Agriculture, Dairy & Animal Science
Dagmara Stepien-Pysniak, Tomasz Hauschild, Marta Dec, Agnieszka Marek, Renata Urban-Chmiel, Urszula Kosikowska
Summary: Bacterial infections of yolk sacs in chicks can lead to high mortality rates and economic losses in poultry production. This study aimed to characterize enterococci isolated from yolk sac infections in broiler chickens from Poland and the Netherlands, finding important virulence factors and genetic relatedness among isolates.
Article
Biochemistry & Molecular Biology
Wiriya Maisat, Koichi Yuki
Summary: This study investigated the role of ion channels/transporters and volatile anesthetics in biofilm formation by E. faecalis. The chloride transporter mutant showed reduced biofilm formation compared to the parental strain, while isoflurane exposure enhanced biofilm formation. These findings provide a better understanding of the underlying mechanisms of E. faecalis biofilm formation and highlight the potential impact of ion transporters and volatile anesthetics on this process.
Article
Gastroenterology & Hepatology
Natalia Nunez, Aurelie Derre-Bobillot, Nicolas Trainel, Goran Lakisic, Alexandre Lecomte, Francoise Mercier-Nome, Anne-Marie Cassard, Helene Bierne, Pascale Serror, Cristel Archambaud
Summary: This study reveals the ability of Enterococcus faecalis to survive and proliferate in hepatocytes and exhibit similar behaviors in kidney cells. This finding is of great significance for understanding pathobiont-driven diseases.
Article
Food Science & Technology
Marlena Golas-Pradzynska, Magdalena Luszczynska, Jolanta Grazyna Rola
Summary: This study investigates the antimicrobial resistance, virulence, and resistance genes of Enterococcus faecalis and Enterococcus faecium isolated from raw goat's and sheep's milk and cheese. The results show that E. faecalis is the dominant species, with high levels of antimicrobial resistance and presence of virulence genes in some strains.
Article
Plant Sciences
Jianyan Qi, Min Gong, Rui Zhang, Yumeng Song, Qian Liu, Hongyan Zhou, Jue Wang, Yufeng Mei
Summary: Tea tree essential oil (TTO) is known for its traditional medicinal uses and earlier research has shown its effectiveness in inhibiting oral microorganisms. This study found that TTO can destroy cell membrane, inhibit biofilm formation, and eliminate mature biofilms of E. faecalis, suggesting its potential as a novel antibacterial drug.
JOURNAL OF ETHNOPHARMACOLOGY
(2021)
Article
Microbiology
Beatriz del Rio, Esther Sanchez-Llana, Noelia Martinez, Maria Fernandez, Victor Ladero, Miguel A. Alvarez
Summary: Enterococci are a diverse group of Gram-positive, lactic acid bacteria that can be found in various environments, including fermented foods, posing a health threat and hospital-acquired infections due to antimicrobial resistance. Bacteriophages are ideal biotechnological weapons for controlling bacterial growth, but require a combination of phages with different host ranges. The isolation of Enterococcus faecalis-infecting bacteriophages from cheese samples adds to the potential arsenal for bacteriophage-based biocontrol of harmful E. faecalis populations.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Veterinary Sciences
Sung Hyun Bae, Sunghyun Yoon, Koeun Kim, Yeong Bin Kim, Young Ju Lee
Summary: The study compared chloramphenicol-resistant Enterococcus faecalis isolated from bulk tank milk of four major dairy companies in Korea. Results showed significant differences in antimicrobial resistance genes and the emergence of resistance among the dairy companies, emphasizing the need for effective surveillance programs at a multidisciplinary level.
VETERINARY SCIENCES
(2021)
Article
Pharmacology & Pharmacy
Mingxin Hu, Shanthini Kalimuthu, Chengfei Zhang, Islam A. A. Ali, Prasanna Neelakantan
Summary: Enterococcus faecalis is a common opportunistic pathogen with high antibiotic tolerance and biofilm-forming ability. Combining trans-cinnamaldehyde with an FDA-approved biosurfactant significantly reduces bacterial viability and biomass of E. faecalis biofilms. This study highlights the potential of the newly developed TC-ASL complex as an anti-biofilm strategy.
Review
Medicine, General & Internal
Masoud Dadashi, Parastoo Sharifian, Nazila Bostanshirin, Bahareh Hajikhani, Narjess Bostanghadiri, Nafiseh Khosravi-Dehaghi, Alex van Belkum, Davood Darban-Sarokhalil
Summary: Based on the analysis of 114 studies worldwide, it was found that the prevalence of antibiotic-resistant E. faecalis and E. faecium to linezolid, tigecycline, and daptomycin is relatively low but increasing.
FRONTIERS IN MEDICINE
(2021)