Article
Immunology
Shubham Chakravarty, Layla Ramos-Hegazy, Abigail Gasparovic, Gregory G. Anderson
Summary: The overexpression of polB significantly reduced T3SS transcription and repressed translation of the master T3SS regulator ExsA. Furthermore, polB does not act through previously described genetic pathways that post-transcriptionally regulate ExsA.
MICROBES AND INFECTION
(2021)
Review
Microbiology
Megan R. O'Malley, Jeffrey C. Anderson
Summary: P. syringae utilizes the T3SS to promote virulence, but these genes are not constitutively expressed and must be induced during infection. Recent research has identified plant-derived amino acids and organic acids that induce T3SS-inducing genes in P. syringae. The regulatory machinery governing T3SS deployment includes master regulators HrpRS and HrpL, as well as environmental factors.
Review
Immunology
Albane Jouault, Alessandra Mattos Saliba, Lhousseine Touqui
Summary: This mini-review discusses how the type III secretion system (T3SS) of Pseudomonas aeruginosa and the bacterial effectors secreted through this system can activate and manipulate the host immune response, promoting the survival and establishment of the pathogen.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Plant Sciences
Xiaoli Liu, Jun Cai, Xiaoxu Li, Feng Yu, Dousheng Wu
Summary: The article hypothesizes that type III effectors manipulate the microbiota composition by mediating ternary pathogen-plant-microbiota interactions, providing new insights for studying the biological functions of type III effectors and developing new strategies for plant disease control.
PLANT CELL AND ENVIRONMENT
(2022)
Article
Fisheries
Zhen Tao, Haoda Ye, Chaozheng Zhang, Suming Zhou, Kequan Wang, Mingming Zhang, Jianjun Xie, Hetron Mweemba Munang'andu, Cheng Xu, Pengcheng Wang, Xiaojun Yan
Summary: In this study, a novel two-component system PvgAS has been identified in P. plecoglossicida, which regulates the expression of T6SS-1 and plays a crucial role in bacterial pathogenicity. This finding provides important insights into the understanding of the pathogenic mechanisms of P. plecoglossicida and potential therapeutic interventions against this bacterium.
Article
Biotechnology & Applied Microbiology
Yushan Xia, Congjuan Xu, Dan Wang, Yuding Weng, Yongxin Jin, Fang Bai, Zhihui Cheng, Oscar P. Kuipers, Weihui Wu
Summary: YbeY is a highly conserved RNase in bacteria that plays essential roles in rRNA processing, small RNA regulation, and bacterial responses to environmental stresses. Mutation of the ybeY gene in Pseudomonas aeruginosa leads to dysregulation of secretion system gene expression and biofilm formation, indicating the pleiotropic functions of YbeY in bacterial regulation.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2021)
Article
Microbiology
Jing Wang, Yuan Luo, Yilin Gu, Hai-Lei Wei
Summary: Pseudomonas fluorescens 2P24 is a plant growth-promoting rhizobacterium with a complete SPI-1 type III secretion system gene cluster. HilA and InvF were identified as two transcriptional regulator homologs within the SPI-1 T3SS gene cluster, regulating gene expression positively and up-regulating genes, respectively. The SPI-1 mutants of 2P24 showed different phenotypes in resistance against Fusarium graminearum, biofilm formation, and chemotactic responses to glucose compared to the wild type strain.
FRONTIERS IN MICROBIOLOGY
(2021)
Review
Cell Biology
Flavia Viana, Shruthi Sachidanandan Peringathara, Arshad Rizvi, Gunnar N. Schroeder
Summary: Proteases are powerful enzymes that irreversibly cleave peptide bonds to control critical cell fate decisions. Bacterial pathogens deliver protease effectors through secretion systems to manipulate host processes, with functional diversity reflecting adaptations to specific hosts. Emerging concepts like effector-triggered immunity provide insights into host defense against protease attacks.
CELLULAR MICROBIOLOGY
(2021)
Article
Immunology
Ines Serrano Pereira, Sara Vilela Pais, Vitor Borges, Maria Jose Borrego, Joao Paulo Gomes, Luis Jaime Mota
Summary: A study revealed that the CteG effector protein of Chlamydia trachomatis is involved in the lytic exit of host cells. Cells infected with wild-type C. trachomatis released more infectious chlamydiae in the culture supernatant compared to cells infected with a CteG-deficient strain. CteG also played a role in host cell cytotoxicity. Furthermore, CteG and Pgp4 were found to participate in a cascade of events leading to host cell lysis and release of infectious chlamydiae.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Lu-Lu He, Xin Wang, Dylan O'Neill Rothenberg, Xiaoli Xu, Hai-Hong Wang, Xin Deng, Zi-Ning Cui
Summary: In this study, a high-throughput screening reporter system was constructed to screen for imidazole, oxazole, and thiazole compounds. The screening identified three compounds that significantly inhibited the activity of hrpW and hrpL gene promoters in P. syringae without affecting its growth. These compounds were found to suppress the expression of T3SS genes and inhibit the pathogenicity of P. syringae in tomato and bean. The findings suggest the potential of novel P. syringae T3SS inhibitors for disease prevention and control.
PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Charlotta Sundin, Michael Saleeb, Sara Spjut, Liena Qin, Mikael Elofsson
Summary: The study identified small molecule binders of PcrV through surface plasmon resonance screening, with subsequent analysis showing their protective effects on macrophages in a P. aeruginosa cell-based infection assay. Treatment of P. aeruginosa infections is challenging due to resistance factors and virulence systems, making virulence blocking molecules targeting PcrV valuable for development of next generation antibacterials.
Review
Microbiology
Elizabeth A. Rucks
Summary: Type III secretion systems (T3SS) are utilized by Gram-negative pathogens to deliver effector proteins into target eukaryotic cells for manipulating cell functions and enhancing pathogenesis. This review will discuss the history, biochemical characterization, and function of chlamydial T3SS, as well as the use of heterologous/surrogate models for studying it. The review will also cover the history of chlamydial effectors and recent advances in the field.
MICROBIOLOGY AND MOLECULAR BIOLOGY REVIEWS
(2023)
Article
Multidisciplinary Sciences
Eduardo Soto, Jorge E. Galan, Marfa Lara-Tejero
Summary: In this study, an extensive in vivo cross-linking strategy aided by structure modeling was used to investigate the sorting platform complex of type III secretion systems. The assembly process of this bacterial structure was mapped using identified cross-links as signatures for pairwise intersubunit interactions and systematic genetic deletions. Insights generated by this study could be used for the rational development of antivirulence strategies against medically important bacterial pathogens.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Microbiology
Antoine Zboralski, Adrien Biessy, Martin Filion
Summary: The distribution and functions of Type III secretion systems (T3SSs) in two important bacterial groups, nitrogen-fixing rhizobia and plant-beneficial Pseudomonas spp., are discussed. T3SSs and their effectors play important roles in legume-rhizobia symbiosis and in plant-beneficial Pseudomonas spp. The diversity of T3SSs in plant-beneficial bacteria suggests their significant roles in interkingdom interactions in the rhizosphere.
Article
Microbiology
Hao Chen, Xuetao Gong, Zheng Fan, Yushan Xia, Yongxin Jin, Fang Bai, Zhihui Cheng, Xiaolei Pan, Weihui Wu
Summary: Rising antimicrobial resistance poses a severe threat to human health, and understanding bacterial regulation of virulence and antimicrobial resistance determinants is urgently needed for developing new antimicrobial strategies. This study demonstrates that a mutation in citrate synthase gltA increases the expression of type III secretion system (T3SS) genes and antibiotic tolerance in Pseudomonas aeruginosa. The stringent response is activated in the gltA mutant, and restoring the antibiotic tolerance and T3SS gene expression to wild-type level requires deletion of the (p)ppGpp synthetase gene relA. Furthermore, increased intracellular cAMP level in the gltA mutant due to the stringent response enhances the expression of the T3SS master regulator gene exsA. This work reveals the important roles of GltA in metabolism, antibiotic tolerance, and virulence, as well as a novel regulatory mechanism of the stringent response-mediated regulation of the T3SS in P. aeruginosa.
MICROBIOLOGY SPECTRUM
(2023)
Article
Microbiology
Dacie R. Bridge, Jeannette M. Whitmire, Jeremy J. Gilbreath, Eleanor S. Metcalf, D. Scott Merrell
INTERNATIONAL JOURNAL OF MEDICAL MICROBIOLOGY
(2015)
Review
Infectious Diseases
Stephanie L. Servetas, Dacie R. Bridge, D. Scott Merrell
CURRENT OPINION IN INFECTIOUS DISEASES
(2016)
Article
Immunology
Dacie R. Bridge, Karen H. Martin, Elizabeth R. Moore, Wendy M. Lee, James A. Carroll, Claudia L. Rocha, Joan C. Olson
INFECTION AND IMMUNITY
(2012)
Article
Biology
Matthew J. Novotny, Dacie R. Bridge, Karen H. Martin, Scott A. Weed, Robert B. Wysolmerski, Joan C. Olson
Review
Gastroenterology & Hepatology
Dacie R. Bridge, D. Scott Merrell
Article
Ophthalmology
Lee Wiley, Dacie R. Bridge, Lee A. Wiley, J. Vernon Odom, Thomas Elliott, Joan C. Olson
INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE
(2012)