Article
Biotechnology & Applied Microbiology
Jessika Nowak, Sandra B. Visnovsky, Andrew R. Pitman, Cristina D. Cruz, Jon Palmer, Graham C. Fletcher, Steve Flint
Summary: Listeria monocytogenes is a common foodborne pathogen that contaminates food during processing, likely due to biofilm formation. Multiple genes have been identified to be involved in biofilm formation by this pathogen, impacting biofilm structure.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Hellen Buijze, Volker Brinkmann, Robert Hurwitz, Anca Dorhoi, Stefan H. E. Kaufmann, Gang Pei
Summary: Human guanylate-binding proteins (hGBPs) are associated with intracellular Mycobacterium tuberculosis (Mtb) and Listeria monocytogenes (Lm) through disruption of phagosomal membranes. The formation of puncta structures by hGBP1 is dependent on GTP-binding and isoprenylation and is required for endolysosomal integrity and repair.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Food Science & Technology
Haoqiu Wang, Mengyao Feng, Tanveer Muhammad Anwar, Wenqin Chai, Abdelaziz Ed-Dra, Xiamei Kang, Kalliopi Rantsiou, Corinna Kehrenberg, Min Yue, Yan Li
Summary: This study aimed to determine the difference in survival and antimicrobial susceptibility of L. monocytogenes and other Listeria species under different food processing environment stressors. The results showed that L. monocytogenes strains were more tolerant to environmental stresses. Additionally, anaerobic incubation after exposure to stressors rendered Listeria species more resistant to antimicrobials. Different stressors induced an increase in the minimum inhibitory concentrations of certain antimicrobials. These findings have important implications for controlling Listeria species in the food industry.
FRONTIERS IN SUSTAINABLE FOOD SYSTEMS
(2023)
Article
Food Science & Technology
Carolina Ibarguren, M. Virginia Guitian, Romina M. Lenz, Soria M. Cecilia, M. Carina Audisio
Summary: Listeria monocytogenes is a foodborne pathogen that can survive in harsh food processing environments. The use of bacteriocins as a natural alternative to control this pathogen has been hindered by the emergence of resistant strains. This study compares the properties of susceptible and resistant strains, including growth, antibiotic susceptibility, cell morphology, virulence factor expression, and biofilm formation. The results contribute to the understanding of bacteriocin-resistance development in L. monocytogenes and its implications for the use of these peptides as biopreservatives.
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY
(2022)
Article
Microbiology
Lixia Wang, Chunhui Ji, Xianzhu Xia, Xuepeng Cai, Qingling Meng, Jun Qiao
Summary: This study investigated the regulatory roles and mechanisms of sRNA Rli43 in Listeria monocytogenes. The results showed that Rli43 plays important roles in environmental adaptation, biofilm formation, and virulence modulation in L. monocytogenes.
Article
Microbiology
Robert Hanes, Fangyuan Zhang, Zuyi Huang
Summary: Listeria monocytogenes is a dangerous foodborne pathogen that poses a significant threat to the food industry. A data analysis approach was used to study its stress response, virulence, and antimicrobial resistance by creating protein interaction networks. Key proteins were identified, including sigB, flaA, cheA, cheY, and lmo0693, which show high potential as targets for combating L. monocytogenes. These findings offer new directions for improving food preservation methods and developing treatments for L. monocytogenes.
Article
Food Science & Technology
Zhipeng Gao, Weiming Zhong, Ting Liu, Tianyu Zhao, Jiajing Guo
Summary: In this study, the response of Listeria monocytogenes exposed to linalool was explored using iTRAQ-based quantitative proteomics analysis to uncover the mode of action and drug targets of linalool against LM. A total of 445 differentially expressed proteins were identified, with 31 significantly enriched gene ontology functional categories and 60 significantly enriched biological pathways. The analysis suggested that cell membranes, cell walls, nucleoids, and ribosomes might be the targets of linalool against LM.
Article
Microbiology
Damayanti Chakravarty, Gyan Sahukhal, Mark Arick II, Morgan L. Davis, Janet R. Donaldson
Summary: This study analyzed the differences in transcript levels produced by Listeria monocytogenes under conditions mimicking the gastrointestinal tract, revealing that anaerobic environments have an impact on gene transcription, particularly under acidic conditions. These findings provide comprehensive information on how L. monocytogenes responds to the gastrointestinal tract.
FRONTIERS IN MICROBIOLOGY
(2021)
Review
Microbiology
Natalia Wiktorczyk-Kapischke, Krzysztof Skowron, Katarzyna Grudlewska-Buda, Ewa Walecka-Zacharska, Jakub Korkus, Eugenia Gospodarek-Komkowska
Summary: Listeria monocytogenes is a Gram-positive, facultatively anaerobic bacterium that poses a significant threat to human health, especially to vulnerable populations such as the elderly, pregnant women, and newborns. Its ability to adapt and thrive in various environmental conditions, particularly in food processing environments, highlights the importance of understanding its mechanisms of adaptation for effective control in the food industry.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Food Science & Technology
Yuan Wang, Linjun Sun, Lili Hu, Zhen Wang, Xiang Wang, Qingli Dong
Summary: According to the study, the nutrition status affects the adhesion ability and biofilm formation of Listeria monocytogenes. Nutrient deficiency can enhance the adhesion and biofilm formation of the bacteria, and also lead to changes in the expression of critical biofilm-associated genes. These findings are important for understanding the spread and control of Listeria in food processing.
FOOD RESEARCH INTERNATIONAL
(2022)
Article
Biotechnology & Applied Microbiology
J. Nowak, S. B. Visnovsky, C. D. Cruz, G. C. Fletcher, A. H. M. van Vliet, D. Hedderley, R. Butler, S. Flint, J. Palmer, A. R. Pitman
Summary: The study revealed that biofilm formation and gallidermin resistance of L. monocytogenes are influenced by the mprF gene, with its inactivation leading to enhanced biofilm formation and abiotic surface attachment but compromising invasiveness.
JOURNAL OF APPLIED MICROBIOLOGY
(2021)
Article
Immunology
Changyong Cheng, Feng Liu, Haobo Jin, Xiangfei Xu, Jiali Xu, Simin Deng, Jing Xia, Yue Han, Lei Lei, Xian Zhang, Houhui Song
Summary: Research shows that DegU plays a vital role in heat resistance in L. monocytogenes, sensing heat stress through autoregulation and upregulating the production of heat-shock proteins. DegU can also directly regulate the expression of specific operons, thus regulating heat resistance in L. monocytogenes.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Cristina Serra-Castello, Ilario Ferrocino, Anna Jofre, Luca Cocolin, Sara Bover-Cid, Kalliopi Rantsiou
Summary: The combination of lactate as an antimicrobial agent and high-pressure processing (HPP) as a lethal treatment is an effective strategy for controlling L. monocytogenes in cooked meat products. The molecular mechanisms underlying the protective effect of lactate involve promoting shifts in pathogen metabolism and membrane properties, enhancing resistance to HPP. This study provides insights into the adaptive responses of L. monocytogenes to lactate and HPP, contributing to the understanding of the piezo-protective effect of lactate.
Review
Food Science & Technology
Minmin Hu, Qingli Dong, Yangtai Liu, Tianmei Sun, Mingliang Gu, Huajian Zhu, Xuejuan Xia, Zhuosi Li, Xiang Wang, Yue Ma, Shuo Yang, Xiaojie Qin
Summary: This study aimed to conduct a meta-analysis of Listeria monocytogenes response to sanitizer treatments. The results showed that environmental factors such as sanitizer concentration, temperature, and treatment time affected the average log-reduction of L. monocytogenes. L. monocytogenes exhibited strong resistance to citric acid and sodium hypochlorite but had low resistance to electrolyzed water. The physical and chemical properties of the contaminated or inoculated matrix or surface also influenced the sanitizer effectiveness.
Article
Food Science & Technology
Christos Toliopoulos, Efstathios Giaouris
Summary: Listeria monocytogenes is a foodborne pathogen that can form biofilms on various surfaces and express different genes in biofilm and planktonic cells, which could affect its stress tolerance and virulence. This study investigated the expression of ten key stress response and/or virulence-related genes in three different strains of L. monocytogenes and found strain-dependent differential gene expression between biofilm and planktonic cells, suggesting the importance of considering strain variability in studies and eradication methods of pathogenic biofilms.
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY
(2023)
Article
Immunology
Juan Zhang, Shuyi Wang, Tjakko Abee, Stijn van der Veen
Summary: This study highlights the importance of base excision repair (BER) for Listeria monocytogenes virulence and survival during host colonization. By deleting specific DNA glycosylases and apurinic/apyrimidinic endonucleases, researchers found that certain mutants were attenuated in virulence in a mouse model and showed reduced colonization in the liver and spleen.
JOURNAL OF INFECTIOUS DISEASES
(2021)
Article
Infectious Diseases
Jing Yan, Yan Chen, Fan Yang, Xia Ling, Shengnan Jiang, Feng Zhao, Yunsong Yu, Stijn van der Veen
Summary: This study found a high percentage of ceftriaxone-resistant FC428 clone among clinical isolates from a single hospital in Hangzhou, China, with seven isolates displaying high-level ceftriaxone resistance and treatment failure potentially linked to initial prescription of oral cephalosporins. Retreatment with a higher dose of IV ceftriaxone appeared successful in these cases.
JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY
(2021)
Article
Biochemistry & Molecular Biology
Yu Chen, Eunice van Pelt-KleinJan, Berdien van Olst, Sieze Douwenga, Sjef Boeren, Herwig Bachmann, Douwe Molenaar, Jens Nielsen, Bas Teusink
Summary: Cells adapt to different conditions by adjusting gene expression to regulate metabolism for optimal fitness, but constraints on the cellular proteome may limit these adaptive strategies. Understanding the impact of proteome constraints on growth rate in nutrient-rich environments can inform strategies for improving fitness in microorganisms.
MOLECULAR SYSTEMS BIOLOGY
(2021)
Article
Biology
Chrats Melkonian, Lucas Fillinger, Siavash Atashgahi, Ulisses Nunes da Rocha, Esther Kuiper, Brett Olivier, Martin Braster, Willi Gottstein, Rick Helmus, John R. Parsons, Hauke Smidt, Marcelle van der Waals, Jan Gerritse, Bernd W. Brandt, Wilfred F. M. Roeling, Douwe Molenaar, Rob J. M. van Spanning
Summary: Researchers used metagenomics and transcriptomics to study microbial dynamics in a 15-year old bioreactor with benzene as the main carbon source. Their findings revealed that most members of the microbial community likely feed on metabolic leftovers or necromass, while only a few are capable of degrading benzene. These benzene degraders are essential for maintaining species richness and ecosystem stability within the community.
COMMUNICATIONS BIOLOGY
(2021)
Article
Genetics & Heredity
Elena G. Lara, Isabelle van der Windt, Douwe Molenaar, Marjon G. J. de Vos, Chrats Melkonian
Summary: A computational approach using metabolic gene annotation was used to rapidly predict putative interactions between members of urinary tract infection microbial communities, revealing that Enteroccus spp. were most complemented in its metabolism by other community members. Experimental supplementation of predicted metabolites confirmed the increase in population density of UTI Enterococcus isolates. This method shows promise as a rapid in silico screening tool for predicting metabolic interactions in microbial communities.
Correction
Biology
Chrats Melkonian, Lucas Fillinger, Siavash Atashgahi, Ulisses Nunes da Rocha, Esther Kuiper, Brett Olivier, Martin Braster, Willi Gottstein, Rick Helmus, John R. Parsons, Hauke Smidt, Marcelle van der Waals, Jan Gerritse, Bernd W. Brandt, Wilfred F. M. Roeling, Douwe Molenaar, Rob J. M. van Spanning
COMMUNICATIONS BIOLOGY
(2021)
Article
Soil Science
Kristof Brenzinger, Ohana Y. A. Costa, Adrian Ho, Guusje Koorneef, Bjorn Robroek, Douwe Molenaar, Gerard Korthals, Paul L. E. Bodelier
Summary: The study demonstrated that steering soil microbiome through application of specific organic amendments can reduce greenhouse gases emissions, increase soil carbon content, and promote crop growth. Organic amendments have the potential to modulate the microbial community in soils, making them more climate-smart compared to conventional use of mineral fertilizers.
BIOLOGY AND FERTILITY OF SOILS
(2021)
Article
Infectious Diseases
Qiang Wang, Xiaofang Tang, Stijn van der Veen
Summary: This case report describes a surgical patient with Mycoplasma hominis bloodstream infection and pneumonia. After unsuccessful treatment with standard antimicrobial therapy, the pathogen was identified through culturing and sequencing, and combination therapy based on antimicrobial susceptibility testing was administered. Importantly, a prostate abscess was identified as a possible primary source of infection.
BMC INFECTIOUS DISEASES
(2022)
Article
Chemistry, Multidisciplinary
Rajesh Varkhedkar, Fan Yang, Rakesh Dontha, Jianglin Zhang, Jiyong Liu, Bernhard Spingler, Stijn van der Veen, Simon Duttwyler
Summary: The identification of an alternative chemical space is crucial for discovering novel antimicrobial lead compounds to address the global challenge of antimicrobial resistance. Boron clusters are being explored due to their unique properties, but their synthesis and derivatization techniques have limitations. A new method was developed to rapidly construct a library of molecules and evaluate their antibacterial activity.
ACS CENTRAL SCIENCE
(2022)
Article
Biochemistry & Molecular Biology
Rosanne Hertzberger, Ali May, Gertjan Kramer, Isabelle van Vondelen, Douwe Molenaar, Remco Kort
Summary: Glycogen in the female lower reproductive tract is an important carbon source for colonization and acidification by vaginal Lactobacillus crispatus. This study characterizes the genetic variation and regulation of the pulA gene, which correlates with the ability to utilize glycogen for growth. The findings suggest that environmental and genetic factors contribute to the variation of Lactobacillus crispatus alpha-glucosidases in the vaginal environment.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Cell Biology
Chunting Hua, Qiaoli Zheng, Jiang Zhu, Siji Chen, Yinjing Song, Stijn van der Veen, Hao Cheng
Summary: This study found that high-risk human papillomavirus 16 infection is associated with activation of the mitogen activated protein kinase (MAPK)/ERK signaling pathway and induction of canonical autophagy. Additionally, the expression of dual-specificity phosphates 5 (DUSP5) is repressed by HPV16 E7. These findings suggest that DUSP5 may serve as a potential therapeutic target for cervical cancer.
OXIDATIVE MEDICINE AND CELLULAR LONGEVITY
(2022)
Article
Microbiology
Shuai Gao, Abidullah Khan, Xuhong Chen, Guohui Xiao, Stijn van der Veen, Yin Chen, Xu'ai Lin
Summary: This study demonstrates that c-di-GMP can stimulate immune responses and reduce MRSA infections by enhancing the secretion of antimicrobial peptides and chemokines. In a mouse skin wound infection model, pre-treatment with c-di-GMP significantly reduced bacterial colonization and promoted inflammatory cell infiltration, making it more difficult for MRSA to establish an infection.
Article
Cell Biology
Jodie H. K. Man, Charlotte A. G. H. van Gelder, Marjolein Breur, Daniel Okkes, Douwe Molenaar, Sophie van der Sluis, Truus Abbink, Maarten Altelaar, Marjo S. van der Knaap, Marianna Bugiani
Summary: This study comprehensively analyzed the cortex of vanishing white matter (VWM) patients using high-resolution mass spectrometry-based proteomics and immunohistochemistry. The findings revealed a large number of differentially expressed proteins in the VWM cortex, forming a major protein interaction network. Gene ontology analysis further indicated enrichment for cellular metabolism, particularly mitochondrial activity. Importantly, the study identified astrocytic involvement in the cortical pathology of VWM, with morphological changes and decreased complexity observed in VWM cortical astrocytes compared to control cells. These astrocytes were found to be immature and non-reactive. These insights into cortical involvement in VWM have significant implications for the development of therapeutic strategies.
Article
Immunology
Lingyu Gao, Zhemin Wang, Stijn van der Veen
Summary: The study elucidated the mechanisms of Neisseria gonorrhoeae resistance to palmitic acid. A stable palmitic acid-resistant strain obtained through serial passage outcompeted its parent strain in a murine infection model, with resistance-related SNPs and determinants enhancing gene expression of the FarAB efflux pump and enzyme activity of the FadD enzyme.
JOURNAL OF INFECTIOUS DISEASES
(2021)
Review
Microbiology
Frank J. Bruggeman, Robert Planque, Douwe Molenaar, Bas Teusink
FEMS MICROBIOLOGY REVIEWS
(2020)
