期刊
MICROBIOLOGY-SGM
卷 154, 期 -, 页码 2680-2688出版社
SOC GENERAL MICROBIOLOGY
DOI: 10.1099/mic.0.2008/019075-0
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资金
- MRC
- Wellcome Trust (UK)
- Marie Curie Research Training Network
- European Molecular Biology Organization
- MRC [G0800148, G0100471] Funding Source: UKRI
- Medical Research Council [G0100471, G0800148] Funding Source: researchfish
Intracellular replication of Salmonella enterica serovar Typhimurium within membrane-bound compartments, called Salmonella-containing vacuoles, depends on the activities of several effector proteins translocated by the Salmonella pathogenicity island 2 (SPI-2)-encoded type III secretion system. The SPI-2 effector protein SseJ shows similarity at the amino acid level to several GDSL lipases with glycerophospholipid : cholesterol acyltransferase (GCAT) activity. In this study, we show that catalytic serine-dependent phospholipase A (PLA) and GCAT activity of recombinant SseJ is potentiated by factor(s) present in HeLa cells, RAW macrophages and Saccharomyces cerevisiae. SseJ activity was enhanced with increasing amounts of, or preincubation with, eukaryotic cell extracts. Analysis of the activating factor(s) shows that it is soluble and heat- and protease-sensitive. We conclude that PLA and GCAT activities of SseJ are potentiated by proteinaceous eukaryotic factor(s).
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