Article
Microbiology
Donghoon Kang, Natalia V. Kirienko
Summary: Pseudomonas aeruginosa is a multidrug-resistant pathogen that causes acute infections in immunocompromised patients, using the siderophore pyoverdine to facilitate infection and virulence. Inhibiting pyoverdine production can reduce pathogen virulence and may be a promising therapeutic target.
Article
Immunology
Jan G. T. Vogel, Joko P. Wibowo, Hillina Fan, Rita Setroikromo, Kan Wang, Alexander Domling, Frank J. Dekker, Wim J. Quax
Summary: This study successfully synthesized chromene-derived inhibitory molecules that competitively inhibit the acylase PvdQ, reducing the formation of pyoverdine in P. aeruginosa and demonstrating a protective effect in an infection model.
MICROBES AND INFECTION
(2022)
Article
Biology
Esraa H. Hamza, Ashraf M. El-Shawadfy, Ayman A. Allam, Wesam A. Hassanein
Summary: In people with cystic fibrosis, chronic lung infections and hospital-acquired pneumonia are caused by Pseudomonas aeruginosa. This bacterium produces siderophores to obtain iron ions for growth and infection. The lasR gene is crucial for the production of siderophores and biofilm formation in P. aeruginosa.
SAUDI JOURNAL OF BIOLOGICAL SCIENCES
(2023)
Article
Infectious Diseases
Kei Ikarashi, Ryo Kutsuna, Junko Tomida, Yoshiaki Kawamura, Yuji Morita
Summary: The study found that MexXY overexpression and deficiency in multidrug-resistant Pseudomonas aeruginosa can impact pyoverdine production, with MexXY deficiency leading to increased pyoverdine production. MexXY-deficient mutants may be more susceptible to iron deficiency, while high-risk clones are usually MexXY overproducers but defective pyoverdine producers.
Review
Biotechnology & Applied Microbiology
Geum-Jae Jeong, Fazlurrahman Khan, Sohail Khan, Nazia Tabassum, Sonu Mehta, Young-Mog Kim
Summary: Pseudmonas aeruginosa is a widespread Gram-negative bacterium responsible for infections in various parts of the body. The development of multiple resistance mechanisms has led to failure of antimicrobial therapy. Inhibition of siderophore production in P. aeruginosa has emerged as a promising therapeutic option to limit their infection.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2023)
Article
Microbiology
Rebecca J. Quinn, Ivana Barraza, Laura Garcia-Dieguez, Camryn Pajon, Lauren E. Krausfeldt, Kerollos Ibrahim, Laura A. Enzinna, Morgan E. Thorn, Omar Tonsi Eldakar, Travis J. A. Craddock, Robert P. Smith
Summary: Understanding environmental factors affecting virulence factor production is important in evolution and medicine. Periodic physical disturbance can impact the production of virulence factors, such as pyoverdine, by altering the spatial structure of bacterial populations. Mathematical modeling predicts that the synthesis rate of pyoverdine and biofilm density determine the amount of pyoverdine produced during disturbance.
Article
Biochemistry & Molecular Biology
Rafael Amieva, Teresa Gil-Gil, Jose Luis Martinez, Manuel Alcalde-Rico
Summary: Multidrug efflux pumps are crucial for antibiotic resistance in bacteria, but they also modulate other aspects of bacterial physiology, such as quorum sensing and iron homeostasis.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Microbiology
Thais P. Mello, Iuri C. Barcellos, Michaela Lackner, Marta H. Branquinha, Andre L. S. Santos
Summary: In recent years, researchers have investigated the interactions between bacteria and fungi cohabiting and/or responsible for human pathologies. Pseudomonas aeruginosa, a Gram-negative bacterium, and fungal species belonging to the Scedosporium/Lomentospora genera are commonly found in patients with cystic fibrosis. The present study explored the inhibitory effect of bioactive molecules secreted by P. aeruginosa on Scedosporium/Lomentospora species, revealing the complex mechanisms behind this phenomenon.
Article
Microbiology
Derek C. K. Chan, Lori L. L. Burrows
Summary: Iron is essential for bacterial growth but its poor solubility hampers this process. Bacteria produce siderophores to bind iron and overcome this limitation. Pseudomonas aeruginosa, an opportunistic pathogen, has multiple transporters that recognize its own siderophores as well as those produced by other bacteria or fungi, giving it a competitive advantage.
Article
Agricultural Engineering
Chunzhe Lu, Nina Leitner, Rene H. Wijffels, Vitor A. P. Martins dos Santos, Ruud A. Weusthuis
Summary: This study developed a biocatalyst for the production of mcl-diols from n-alkanes under mild conditions. The engineered strain P. putida KT2440, with overexpression of Est12, can efficiently hydrolyze esterified diols, enabling the production of mcl-diols.
BIORESOURCE TECHNOLOGY
(2022)
Review
Biology
Ghassan Ghssein, Zeinab Ezzeddine
Summary: This study summarizes the important characteristics of three metallophores produced by Pseudomonas aeruginosa (pyoverdine, pyochelin, and pseudopaline), including their biosynthesis process, secretion, metal uptake, and genetic regulation.
Article
Microbiology
Donghoon Kang, Alexey Revtovich, Alexander E. Deyanov, Natalia Kirienko, Patricia A. Bradford
Summary: Pseudomonas aeruginosa is a multidrug-resistant pathogen that causes various infections, and exploring new therapeutic avenues, such as targeting virulence factors, is crucial. Gallium(III) nitrate has been recognized for inhibiting pathogen growth and biofilm formation, but the effectiveness can be limited by pyoverdine. Inhibitors of pyoverdine, such as 5-fluorocytosine and tetracycline, can synergize with gallium nitrate to inhibit P. aeruginosa growth and biofilm formation.
Article
Biochemical Research Methods
Forster Anne, Graulier Gwenaelle, Schalk Isabelle, Fechter Pierre
Summary: Pseudomonas aeruginosa (PA) is a common cause of chronic infections, especially in cystic fibrosis patients. PA adapts to the lung environment and treatments by producing and secreting siderophores. We improved a cloning method to study PA's interaction with other bacteria, particularly in competitive environments. This research helps differentiate intra and inter-specific competitions and highlights the importance of bacterial species in siderophore production adaptation.
JOURNAL OF MICROBIOLOGICAL METHODS
(2023)
Article
Biochemistry & Molecular Biology
Alba Arranz San Martin, Steffen Lorenz Drees, Susanne Fetzner
Summary: The opportunistic pathogen Pseudomonas aeruginosa utilizes quorum sensing to regulate its virulence, with the Pseudomonas quinolone signal PQS playing a key role. Certain 3-hydroxy-4(1H)-quinolone 2,4-dioxygenases have been identified as quorum quenching enzymes, breaking down PQS to reduce pathogenic virulence. The activity of HQD from Streptomyces bingchenggensis was unexpectedly stabilized by culture supernatants of P. aeruginosa, indicating an interaction with the pathogen's outer membrane vesicles.
Article
Biochemistry & Molecular Biology
Simon Ernst, Almuth Maehrlein, Niklas H. Ritzmann, Steffen L. Drees, Susanne Fetzner
Summary: Many natural products contain N-O functional groups that play a crucial role in biological activity. This study presents three flavoprotein N-hydroxylases that belong to a distinct phylogenetic branch within the GR2-fold FPMOs, expanding the knowledge on the catalytic repertoire of GR2-type FPMOs and providing a basis for the discovery of other nitrogen functionalizing reactions.
Correction
Oncology
Ilaria Romito, Manuela Porru, Maria Rita Braghini, Luca Pompili, Nadia Panera, Annalisa Crudele, Daniela Gnani, Cristiano De Stefanis, Marco Scarsella, Silvia Pomella, Stefano Levi Mortera, Emmanuel de Billy, Adrian Libenzio Conti, Valeria Marzano, Lorenza Putignani, Manlio Vinciguerra, Clara Balsano, Anna Pastore, Rossella Rota, Marco Tartaglia, Carlo Leonetti, Anna Alisi
JOURNAL OF EXPERIMENTAL & CLINICAL CANCER RESEARCH
(2022)
Article
Biochemical Research Methods
Stefano Levi Mortera, Pamela Vernocchi, Ilaria Basadonne, Alessandro Zandona, Marco Chierici, Martina Durighello, Valeria Marzano, Simone Gardini, Antonio Gasbarrini, Andrea Urbani, Stefano Vicari, Paola Roncada, Cesare Furlanello, Paola Venuti, Lorenza Putignani
Summary: In recent years, studies have shown an increasing relationship between neurodevelopmental disorders and the microbial communities of the intestinal tract. This research utilized a novel metaproteomic approach to investigate the gut microbiota of children with Autism Spectrum Disorders (ASD) compared to relatives and age-matched controls, revealing distinctive functional differences.
JOURNAL OF PROTEOMICS
(2022)
Review
Immunology
Payam Behzadi, Aga Syed Sameer, Saniya Nissar, Mujeeb Zafar Banday, Mario Gajdacs, Herney Andres Garcia-Perdomo, Kulsum Akhtar, Marina Pinheiro, Peter Magnusson, Meysam Sarshar, Cecilia Ambrosi
Summary: Interleukins (ILs), as important members of cytokines, play a crucial role in immune responses. They directly affect immune cell growth, activation, and differentiation, and contribute to inflammatory and anti-inflammatory responses by interacting with a wide range of receptors. Mutations or polymorphisms in IL genes can have significant biological consequences and be associated with various diseases.
JOURNAL OF IMMUNOLOGY RESEARCH
(2022)
Editorial Material
Microbiology
Payam Behzadi, Cecilia Ambrosi, Daniela Scribano, Stefania Zanetti, Meysam Sarshar, Mario Gajdacs, Matthew Gavino Donadu
FRONTIERS IN MICROBIOLOGY
(2022)
Review
Immunology
Antonia Piazzesi, Lorenza Putignani
Summary: This article reviews the impact of helminths and gut microbiota on childhood development and pediatric diseases, and discusses how helminths enact changes in their host through gut microbes. The modifications of gut microbiota induced by helminths are of great importance in overcoming parasitic infections and treating inflammatory diseases. Deepening the understanding of helminth-microbiota interactions may lead to novel and more effective therapeutic strategies for childhood disorders.
PARASITE IMMUNOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Elisa Fardelli, Massimiliano Lucidi, Michael Di Gioacchino, Shadi Bashiri, Luca Persichetti, Giulia Capecchi, Tecla Gasperi, Armida Sodo, Paolo Visca, Giovanni Capellini
Summary: This article investigates the bio-physical mechanisms of the membrane of two A. baumannii strains during dehydration and reveals a decoupling between the phase transition from liquid crystalline to gel phase and evidence of cell lysis. The findings highlight the core mechanism of A. baumannii's resistance against dehydration and the differences in drought resistance between strains.
BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES
(2022)
Article
Biochemistry & Molecular Biology
Stefano Levi Mortera, Valeria Marzano, Pamela Vernocchi, Maria Cristina Matteoli, Valerio Guarrasi, Simone Gardini, Federica Del Chierico, Novella Rapini, Annalisa Deodati, Alessandra Fierabracci, Stefano Cianfarani, Lorenza Putignani
Summary: Recent research has shown a correlation between the composition of the human gut microbiota and the onset of type 1 diabetes. In this study, a comprehensive metaproteomic investigation was conducted on children with type 1 diabetes, revealing potential gut microbiota features related to the severity of the disease.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Bruno Tilocca, Alessio Soggiu, Federica Iavarone, Viviana Greco, Lorenza Putignani, Maria Vittoria Ristori, Gabriele Macari, Anna Antonella Spina, Valeria Maria Morittu, Carlotta Ceniti, Cristian Piras, Luigi Bonizzi, Domenico Britti, Andrea Urbani, Daniel Figeys, Paola Roncada
Summary: This study explored the microbiota of a typical raw goat milk cheese at various ripening timepoints and depths using 16S rRNA gene sequencing and metaproteomics. The results showed a stable microbiota ecology across the selected ripening timepoints, supporting the microbiologically driven fermentation of goat milk products. The compositional and functional differences of the surface and core microbiota were highlighted, showing their roles in maintaining biosafety and contributing to the health benefits and taste of goat cheese.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Anatomy & Morphology
Sofia Canciello, Miranda Parisi, Massimiliano Lucidi, Paolo Visca, Gabriella Cincotti
Summary: Gram staining is used to differentiate bacteria into gram-positive and gram-negative based on their cell wall constituents and coloring. Gram variability refers to the uneven distribution of pink and violet cells within the same bacterial species. We have developed an ImageJ-based pipeline to quantify the gram variability in Acinetobacter baumannii.
MICROSCOPY RESEARCH AND TECHNIQUE
(2023)
Article
Pharmacology & Pharmacy
Leila Mousavifar, Meysam Sarshar, Clarisse Bridot, Daniela Scribano, Cecilia Ambrosi, Anna Teresa Palamara, Gerard Vergoten, Benoit Roubinet, Ludovic Landemarre, Julie Bouckaert, Rene Roy
Summary: A small library of FimH antagonists based on previously described C-linked allyl alpha-D-mannopyranoside was synthesized. Two new members with sub nanomolar affinity were identified and shown to have improved binding compared to previously published analogs. The crystal structure of FimH co-crystallized with one of the antagonists revealed its binding mode, and the antagonists were effective in decreasing bacterial adhesion to human bladder epithelial cells.
Article
Biochemistry & Molecular Biology
Lucrezia Laterza, Lorenza Putignani, Carlo Romano Settanni, Valentina Petito, Simone Varca, Flavio De Maio, Gabriele Macari, Valerio Guarrasi, Elisa Gremese, Barbara Tolusso, Giulia Wlderk, Maria Antonia Pirro, Caterina Fanali, Franco Scaldaferri, Laura Turchini, Valeria Amatucci, Maurizio Sanguinetti, Antonio Gasbarrini
Summary: This study investigated the effectiveness of gut microbiota modulation for enhancing recovery after COVID-19. A pilot interventional study was conducted with twenty recently recovered COVID-19 patients to explore the impact of a mixed probiotic containing Lactobacilli, Bifidobacteria, and Streptococcus thermophilus on gastrointestinal symptoms, inflammation, intestinal barrier integrity, and gut microbiota profile. Various markers and computational algorithms were utilized to evaluate the outcomes before and after 8 weeks of probiotic supplementation. The results showed a significant reduction in inflammatory markers and an increase in Bifidobacteria at T-1, indicating potential anti-inflammatory effects and the influence of probiotics on gut microbiota composition.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Microbiology
Diletta Collalto, Alessandra Fortuna, Paolo Visca, Francesco Imperi, Giordano Rampioni, Livia Leoni
Summary: Co-administration of colistin and clofoctol enhances the bactericidal effect of colistin and reduces the minimum inhibitory concentration (MIC) of colistin in resistant strains. This study suggests the potential development of inhaled clofoctol-colistin formulations for the treatment of difficult-to-treat airway infections caused by drug-resistant Gram-negative pathogens.
MICROBIOLOGY SPECTRUM
(2023)
Article
Engineering, Electrical & Electronic
Miranda Parisi, Massimiliano Lucidi, Paolo Visca, Gabriella Cincotti
Summary: This article reviews various super-resolution microscopy techniques utilized by microbiologists to investigate bacterial cell morphology and functions. The achieved resolution is discussed, with a focus on fluorophore selection and other critical imaging requirements. Recent applications are described, which have provided novel insights into bacterial cellular structures. The pivotal role of super-resolution microscopy in addressing antimicrobial resistance is emphasized.
IEEE JOURNAL OF SELECTED TOPICS IN QUANTUM ELECTRONICS
(2023)
Article
Microbiology
Marta Mellini, Morgana Letizia, Lorenzo Caruso, Alessandra Guiducci, Carlo Meneghini, Stephan Heeb, Paul Williams, Miguel Camara, Paolo Visca, Francesco Imperi, Livia Leoni, Giordano Rampioni
Summary: Quorum sensing allows single cells to synchronize gene expression in bacterial populations, but studies have shown significant cell-to-cell variation in this process, resulting in coexisting subpopulations of active and inactive cells. The molecular mechanisms behind this phenomenon, particularly in the las QS system of Pseudomonas aeruginosa, have not been fully defined.
Article
Microbiology
Lisa Perruzza, Carlo Zagaglia, Laura Vitiello, Meysam Sarshar, Francesco Strati, Martina Pasqua, Fabio Grassi, Mauro Nicoletti, Anna Teresa Palamara, Cecilia Ambrosi, Daniela Scribano
Summary: Intestinal epithelial cells act as the first line of defense against enteric pathogens by using pro-inflammatory programmed cell death to eliminate microbes and create an inflammatory environment. However, pathogens such as Shigella flexneri have evolved the ability to manipulate host cell fate by releasing apyrase to degrade intracellular ATP, preventing cell death and dampening the inflammatory response.
MICROBIOLOGY SPECTRUM
(2023)