Article
Public, Environmental & Occupational Health
Gustavo Marcal Schmidt Garcia Moreira, Sabine Gronow, Stefan Duebel, Marcelo Mendonca, Angela Nunes Moreira, Fabricio Rochedo Conceicao, Michael Hust
Summary: In this study, antibodies against L. monocytogenes were generated using phage display technology, and these antibodies were able to recognize L. monocytogenes with 100% sensitivity and specificity at different concentrations. These findings suggest that phage display-derived antibodies can improve immunodiagnostics for L. monocytogenes.
FRONTIERS IN PUBLIC HEALTH
(2022)
Article
Biochemistry & Molecular Biology
Giulia Magagna, Guido Finazzi, Virginia Filipello
Summary: This study presents a new set of primers for sequencing inlA in both lineages. The presence of premature stop-codon mutations in inlA is correlated with attenuated virulence, and lineage I isolates are most frequently associated with clinical cases. Using these newly designed primers, the presence of inlA PMSCs in food, food processing environments, and clinical isolates was assessed.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Cell Biology
Sae-Hae Kim, Byeol-Hee Cho, Kwang Soon Kim, Yong-Suk Jang
Summary: C5a in the Peyer's patch enhances CD8(+) T cell responses by increasing C5/C5a levels. Single-cell RNA sequencing identifies dendritic cells expressing C5aR and lysozyme as key players in CD8(+) T cell immune induction.
Article
Food Science & Technology
Anderson C. Camargo, Adelle P. McFarland, Joshua J. Woodward, Luis Augusto Nero
Summary: This study examined the invasion, replication, and spread ability of Listeria monocytogenes isolates from Brazil in human intestinal epithelial cells. The results showed that serotype is a better predictor of cell invasion and spread compared with sample origin, and premature stop codons in the inlA gene were associated with decreased invasion ability in certain strains.
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY
(2022)
Article
Immunology
Alejandro Prados, Lucas Onder, Hung-Wei Cheng, Urs Morbe, Mechthild Lutge, Cristina Gil-Cruz, Christian Perez-Shibayama, Vasiliki Koliaraki, Burkhard Ludewig, George Kollias
Summary: The study reveals that fibroblastic reticular cells (FRCs) in Peyer's patches develop from two separate mesenchymal cell lineages, which converge to support immune cell activation and maintain intestinal homeostasis. This mosaic microenvironment plays a crucial role in controlling intestinal immunity, revealing key stromal cell infrastructures for this process.
Article
Microbiology
Hao-Yu Liu, Antoine Giraud, Cedric Seignez, David Ahl, Feilong Guo, John Sedin, Tomas Walden, Jee-Hwan Oh, Jan Peter van Pijkeren, Lena Holm, Stefan Roos, Stefan Bertilsson, Mia Phillipson
Summary: The study shows that oral administration of the probiotic bacterium Limosilactobacillus reuteri R2LC increases the numbers and effector functions of B cell subsets, leading to enhanced IgA production, altered intestinal microbiota, and protection against inflammation.
Article
Biochemistry & Molecular Biology
Franjo Banovic, Sandrin Schulze, Mobarak Abu Mraheil, Torsten Hain, Trinad Chakraborty, Veronique Orian-Rousseau, Selina Moroniak, Christel Weiss, Hiroshi Ishikawa, Horst Schroten, Rudiger Adam, Christian Schwerk
Summary: The human central nervous system (CNS) is protected by distinct cellular barriers, the blood-brain barrier (BBB) and the blood-cerebrospinal fluid (CFS) barrier (BCSFB), which are formed by different cell types. This study investigated the role of the virulence factor InlF and the intermediate filament protein vimentin in CNS invasion by the Gram-positive bacterium Listeria monocytogenes (Lm). The results showed that while vimentin modulated invasion of Lm into brain microvascular endothelial cells (HBMEC), it did not affect invasion into choroid plexus epithelial cells (HIBCPP). In contrast, InlF was found to be dispensable for invasion in both cell types.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Food Science & Technology
Leonardo Lopes-Luz, Ernandes Silva-Filho, Marcelo Mendonca, Angela Nunes Moreira, Andressa Venceslau, Dienny Rodrigues de Sousa, Tatiana Galvez Sanchez, Rodrigo Scaliante de Moura, Fabricio Rochedo Conceicao, Andre Kipnis, Mariane Martins de Araujo Stefani, Samira Buehrer-Sekula
Summary: This study developed a prototype lateral flow immunochromatographic assay (LFIA) using antibodies against L. monocytogenes InlA and InlB proteins, which can potentially be useful for rapid detection of L. monocytogenes in contaminated food. The LFIA demonstrated sensitivity and specificity in detecting L. monocytogenes and discriminating it from other bacterial species.
JOURNAL OF FOOD SCIENCE AND TECHNOLOGY-MYSORE
(2023)
Review
Immunology
Liu Jinjing, Shulman Ziv
Summary: This article discusses the in vivo activity and mechanisms of antibody affinity maturation and competition for T cell help in germinal centers (GCs) in mucosal lymphoid organs.
CURRENT OPINION IN IMMUNOLOGY
(2022)
Article
Immunology
David S. Donaldson, Barbara B. Shih, Neil A. Mabbott
Summary: Aging leads to a decline in mucosal immunity, increasing susceptibility to infections through the gastrointestinal and respiratory tracts in the elderly. Research has shown down-regulation of FAE- and M cell-related genes in aged mice Peyer's patches, alongside an upregulation of ACE2 expression which may increase susceptibility to SARS-Cov-2 infection. Additionally, key Paneth cell-related genes were reduced in the ileum of aged mice, suggesting potential impacts on gut epithelium regenerative ability and M cell differentiation.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Chemistry, Applied
Quanwei Zhang, Shuang Hao, Lifeng Li, Man Liu, Chuying Huo, Wanrong Bao, Huiyuan Cheng, Hauyee Fung, Tinlong Wong, Wenjie Wu, Pingchung Leung, Shunchun Wang, Ting Li, Ge Zhang, Min Li, Zhongzhen Zhao, Wei Jia, Zhaoxiang Bian, Timothy Mitchison, Jingchao Zhang, Aiping Lyu, Quanbin Han
Summary: Radix Astragali polysaccharide (RAP) can enter Peyer's patches (PPs) and directly contact dendritic cells through the transportation by microfold (M) cells. This interesting transcytosis mechanism might apply to many other immunomodulatory polysaccharides.
CARBOHYDRATE POLYMERS
(2022)
Article
Biochemistry & Molecular Biology
Parisa Asadollahi, Iraj Pakzad, Nourkhoda Sadeghifard, Sobhan Ghafourian, Hossein Kazemian, Vahab Hassan Kaviar, Roohollah Fattahi, Behrooz Sadeghi Kalani
Summary: A multi-epitope protein vaccine was designed using immunoinformatics tools to effectively prevent L. monocytogenes infections. The designed vaccine showed stability, specificity, and antigenicity, holding potential for preventing infections caused by this pathogen.
INTERNATIONAL JOURNAL OF PEPTIDE RESEARCH AND THERAPEUTICS
(2022)
Article
Biochemical Research Methods
Ryan D. Sheldon, Eric H. Ma, Lisa M. DeCamp, Kelsey S. Williams, Russell G. Jones
Summary: The protocol integrates traditional immunology infection models with whole-body metabolite infusion and mass-spectrometry-based metabolomic profiling to evaluate T-cell metabolism. Researchers can assess the metabolic substrate utilization of specific T-cell subpopulations in vivo using this method.
Article
Food Science & Technology
Arkadiusz Jozef Zakrzewski, Wioleta Chajecka-Wierzchowska, Anna Zadernowska
Summary: Ceviche is a traditional marinated raw fish dish commonly consumed in Pacific coastal countries. This study aimed to assess the viability of bacteria isolated from fish after the preparation of ceviche. The results showed that different strains of bacteria had varying survival rates and pathogenic potentials during the preparation process, and certain ingredients in ceviche exhibited antibacterial properties.
Article
Food Science & Technology
Margareti Medeiros, Virgilio Hipolito Lemos de Castro, Ana Lourdes Arrais de Alencar Mota, Marita Gimenez Pereira, Elaine Cristina Pereira De Martinis, Simone Perecmanis, Angela Patricia Santana
Summary: The study aimed to classify L. monocytogenes isolated from various food-related sources in the Federal District of Brazil, sequence their internalin A genes, and evaluate their adhesion and invasion capacity using Caco-2 cells. Results showed that isolates with premature stop codons exhibited higher invasion capacity and distinct adhesion compared to those without stop codons.
FOODBORNE PATHOGENS AND DISEASE
(2021)
Article
Engineering, Biomedical
Daiki Nakagawa, Mariko Nakamura, Shigenori Nagai, Mamoru Aizawa
JOURNAL OF MATERIALS SCIENCE-MATERIALS IN MEDICINE
(2020)
Article
Cell Biology
Marek Wagner, Kafi N. Ealey, Hiroe Tetsu, Tsuyoshi Kiniwa, Yasutaka Motomura, Kazuyo Moro, Shigeo Koyasu
Article
Biochemistry & Molecular Biology
Yoshihisa Kashima, Naoto Nishii, Hidetake Tachinami, Emi Furusawa, Shigenori Nagai, Hiroyuki Harada, Miyuki Azuma
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2020)
Article
Immunology
Masahiro Nagata, Kenji Toyonaga, Eri Ishikawa, Shojiro Haji, Nobuyuki Okahashi, Masatomo Takahashi, Yoshihiro Izumi, Akihiro Imamura, Koichi Takato, Hideharu Ishida, Shigenori Nagai, Petr Illarionov, Bridget L. Stocker, Mattie S. M. Timmer, Dylan G. M. Smith, Spencer J. Williams, Takeshi Bamba, Tomofumi Miyamoto, Makoto Arita, Ben J. Appelmelk, Sho Yamasaki
Summary: The study revealed that metabolites produced by Helicobacter pylori interact with host cholesterol and exacerbate gastritis through C-type lectin receptors. The findings from mutant H. pylori strains lacking specific metabolites showed impaired ability to cause gastritis.
JOURNAL OF EXPERIMENTAL MEDICINE
(2021)
Article
Multidisciplinary Sciences
Risa Nakamura, Akihiro Yoshizawa, Taeko Moriyasu, Sharmina Deloer, Masachika Senba, Mihoko Kikuchi, Shigeo Koyasu, Kazuyo Moro, Shinjiro Hamano
Article
Immunology
Mami Sumiyoshi, Yui Kotani, Yuki Ikuta, Kazutomo Suzue, Madoka Ozawa, Tomoya Katakai, Taketo Yamada, Takaya Abe, Kana Bando, Shigeo Koyasu, Yasunori Kanaho, Toshio Watanabe, Satoshi Matsuda
Summary: Arf family members Arf1 and Arf6 play crucial roles in T cell survival, while Arf1/6 deficiency contributes to alleviating autoimmune diseases.
JOURNAL OF IMMUNOLOGY
(2021)
Article
Multidisciplinary Sciences
Hiroko Ishihama, Ken Ishii, Shigenori Nagai, Hiroaki Kakinuma, Aya Sasaki, Kenji Yoshioka, Tetsuya Kuramoto, Yuta Shiono, Haruki Funao, Norihiro Isogai, Takashi Tsuji, Yasunori Okada, Shigeo Koyasu, Yoshiaki Toyama, Masaya Nakamura, Mamoru Aizawa, Morio Matsumoto
Summary: In order to prevent infections associated with medical implants, a new antibacterial coating material with significant bactericidal activity has been developed, showing success in inhibiting bacterial biofilm formation by immobilizing ionic silver on hydroxyapatite film.
SCIENTIFIC REPORTS
(2021)
Article
Immunology
Xiang Ao, Yue Yang, Takashi Okiji, Miyuki Azuma, Shigenori Nagai
Summary: The study found that MDCs induced during sepsis contribute to immune paralysis, and the PD-1/PD-L1-mediated generation of Gr-1(hi) cells in the early phase of sepsis is crucial for the development of immune paralysis in the later stage.
INFECTION AND IMMUNITY
(2021)
Correction
Cell Biology
Yutaka Kurebayashi, Shigenori Nagai, Ai Ikejiri, Masashi Ohtani, Kenji Ichiyama, Yukiko Baba, Taketo Yamada, Shohei Egami, Takayuki Hoshii, Atsushi Hirao, Satoshi Matsuda, Shigeo Koyasu
Article
Immunology
Hisashi Nomura, Naoko Wada, Hayato Takahashi, Yuko Kase, Jun Yamagami, Shohei Egami, Hisato Iriki, Miho Mukai, Aki Kamata, Hiromi Ito, Hideki Fujii, Tomoyuki Ishikura, Haruhiko Koseki, Takashi Watanabe, Taketo Yamada, Osamu Ohara, Shigeo Koyasu, Masayuki Amagai
Summary: The study reveals that Dsg3-specific B cells in mice do not undergo spontaneous pathogenic class switching in vivo and the induction of pemphigus phenotype is prevented under normal conditions.
JOURNAL OF IMMUNOLOGY
(2022)
Review
Immunology
Marek Wagner, Shigeo Koyasu
Summary: Innate lymphoid cells (ILCs), enriched in barrier tissues like skin, play a role in immune pathways despite lacking antigen receptors found on T and B cells. They are crucial in controlling melanoma and potentially benefiting patients with skin malignancies through their therapeutic potential.
FRONTIERS IN IMMUNOLOGY
(2021)
Review
Immunology
Takuma Misawa, Marek Wagner, Shigeo Koyasu
Summary: ILC2s are tissue-resident immune cells that exist not only in adipose tissue but also in various other tissues such as lung and skin. They can change their functions or distribution depending on the environment and circumstances. This review outlines the recent understanding of ILC2 biology and discusses future directions for ILC2 research, particularly in adipose tissue and metabolic homeostasis.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Multidisciplinary Sciences
Tadashi Takeuchi, Tetsuya Kubota, Yumiko Nakanishi, Hiroshi Tsugawa, Wataru Suda, Andrew Tae-Jun Kwon, Junshi Yazaki, Kazutaka Ikeda, Shino Nemoto, Yoshiki Mochizuki, Toshimori Kitami, Katsuyuki Yugi, Yoshiko Mizuno, Nobutake Yamamichi, Tsutomu Yamazaki, Iseki Takamoto, Naoto Kubota, Takashi Kadowaki, Erik Arner, Piero Carninci, Osamu Ohara, Makoto Arita, Masahira Hattori, Shigeo Koyasu, Hiroshi Ohno
Summary: Insulin resistance is the primary pathophysiology underlying metabolic syndrome and type 2 diabetes. Carbohydrate metabolism by gut microbiota plays a crucial role in insulin resistance, and an increase in host-accessible monosaccharides is associated with insulin resistance and host inflammation.
Article
Immunology
Yue Yang, Shigenori Nagai, Siwen Kang, Yulong Xia, Yohei Kawano, Kensuke Miyake, Hajime Karasuyama, Miyuki Azuma
INTERNATIONAL IMMUNOLOGY
(2020)
Article
Immunology
Carlos Henrique Camargo, Amanda Yaeko Yamada, Andreia Rodrigues de Souza, Claudio Tavares Sacchi, Alex Domingos Reis, Marlon Benedito Nascimento Santos, Denise Brandao de Assis, Eneas de Carvalho, Elizabeth Harummyy Takagi, Marcos Paulo Vieira Cunha, Monique Ribeiro Tiba-Casas
Summary: This study reveals the silent spread of New Delhi metallo-beta-lactamase (NDM) carbapenemase in Brazil, particularly in Enterobacterales other than Klebsiella spp. It highlights the diverse species and coexistence of important resistance genes in these NDM-producing strains. Prompt detection and effective measures are crucial to mitigate the further dissemination of NDM carbapenemase and preserve antimicrobial therapy options in hospital settings.
MICROBIOLOGY AND IMMUNOLOGY
(2024)