Article
Immunology
Sophie Octavia, Ka Lip Chew, Raymond T. P. Lin, Jeanette W. P. Teo
Summary: Global travel has led to the occasional introduction of multidrug-resistant Salmonella Typhi into industrialized countries. Azithromycin-resistant Salmonella Typhi has been detected in Singapore, with 2 isolates likely being locally acquired. Ongoing vigilance and surveillance are necessary to minimize the public health risk posed by this serious pathogen.
EMERGING INFECTIOUS DISEASES
(2021)
Article
Microbiology
Stephy Mol Robinson, Vyshakh Rajachandran, Suchismita Majumdar, Satabdi Saha, Sneha Das, Sujay Chattopadhyay
Summary: Detecting adaptive mutations leading to gene inactivation or loss of function is crucial for understanding the evolution of bacterial virulence and antibiotic resistance. Truncation mutations, which trim the length of encoded proteins, can contribute to gene inactivation. This study analyzed the accumulation of truncation mutations in two serovars of Salmonella, finding distinct sets of core truncated genes in each serovar. These truncation mutations were acquired by different subsets of isolates and were also targeted by convergent amino acid mutations in different serovars, suggesting adaptation. Further research is needed to investigate the impact of adaptive truncation mutations on bacterial virulence evolution.
MICROBIOLOGY SPECTRUM
(2022)
Article
Microbiology
Shawn T. Clark, Kirby Cronin, Antoine J. Corbeil, Samir N. Patel
Summary: This work provides an updated summary of the antimicrobial susceptibility patterns among Salmonella Typhi strains isolated from patients in Ontario, Canada. The emergence and spread of resistant strains have complicated the epidemiology and treatment of typhoid fever. A 10-year retrospective survey showed stable susceptibility patterns for certain drugs but an increase in nonsusceptibility to ciprofloxacin and ceftriaxone, indicating the need for healthcare providers to consider current trends in antimicrobial susceptibility for proper therapy selection.
MICROBIOLOGY SPECTRUM
(2023)
Article
Microbiology
Ashraf Hussain, Eugene Boon Beng Ong, Prabha Balaram, Asma Ismail, Phua Kia Kien
Summary: Our study aimed to investigate the effects of deleting tolC on the adhesion and invasion of HT-29 epithelial cells and THP-1 macrophages by S. Typhi in vitro. Our results showed that the tolC mutant strain had significantly reduced adhesion and invasion rates compared to the wild-type strain in both cell types. Additionally, we observed downregulation of SPI-1 gene expression in the tolC mutant.
FRONTIERS IN MICROBIOLOGY
(2023)
Review
Microbiology
Wan Ratmaazila Wan Wan Makhtar, Izwan Bharudin, Nurul Hidayah Samsulrizal, Nik Yusnoraini Yusof
Summary: The advancement in whole-genome sequencing technology in recent years has revolutionized the study of infectious diseases, providing new insights into various fields related to infectious diseases. By examining the genomes of Salmonella enterica serovar Typhi, researchers have enhanced their understanding of genetic evolution and pathogenic mechanisms, potentially advancing the development of typhoid vaccines and medications.
Review
Microbiology
Maryam Khan, Saba Shamim
Summary: S. Typhi is a pathogen causing typhoid fever, with over 27 million cases and 200,000 deaths globally each year. The pathogen causes infection through various virulence factors, including resistance genes, leading to resistance to multiple broad-spectrum antibiotics. The issue of antibiotic resistance has raised global concern.
Article
Chemistry, Analytical
M. S. Bacchu, M. R. Ali, S. Das, S. Akter, H. Sakamoto, S- Suye, M. M. Rahman, K. Campbell, M. Z. H. Khan
Summary: An electrochemical DNA biosensor was fabricated for the selective identification of S. Typhi in real samples. The biosensor showed a low limit of detection and the ability to be reused multiple times. The experimental results demonstrated the high feasibility and accuracy of using this biosensor for detecting S. Typhi in clinical and food samples.
ANALYTICA CHIMICA ACTA
(2022)
Article
Immunology
Hao Tang, Ziyang Zhan, Xiucheng Liu, Ying Zhang, Xinxiang Huang, Min Xu
Summary: Propionate, a major constituent of short chain fatty acids, has been found to reduce the viability of Salmonella enterica serovar Typhi (S. Typhi) in macrophages through propionylation of PhoP. This modification decreases the phosphorylation and DNA-binding ability of PhoP, resulting in reduced intracellular survival of S. Typhi. These findings provide a potential molecular mechanism by which propionate affects the virulence of S. Typhi.
MICROBIAL PATHOGENESIS
(2023)
Article
Immunology
Debayan Ganguli, Swarnali Chakraborty, Suparna Chakraborty, Ananda Pal, Animesh Gope, Santasabuj Das
Summary: Antibiotic resistance is a major threat to public health worldwide. This study investigates the temporary and reversible resistance induced by antibiotic treatment, specifically focusing on the development of L-form bacteria in response to ampicillin. The researchers cultured ampicillin-sensitive Salmonella enterica serovar Typhi Ty2 strain in increasing concentrations of ampicillin over 14 days, resulting in the development of an L-form strain with altered characteristics and increased pathogenicity. These findings highlight the potential for drug escape and cell-to-cell spread of bacteria during antibiotic treatment and the need for new approaches to reduce infection reservoirs.
INFECTION AND IMMUNITY
(2022)
Article
Microbiology
Jessica N. N. Uwanibe, Tolulope A. A. Kayode, Paul E. E. Oluniyi, Kazeem Akano, Idowu B. B. Olawoye, Chinedu A. A. Ugwu, Christian T. T. Happi, Onikepe A. A. Folarin
Summary: This study aims to elucidate the role of asymptomatic carriers in the transmission and persistence of typhoid fever in Nigeria and other endemic countries. The findings show a high seroprevalence of Salmonella Typhi in healthy children, but no carriage, indicating an inability to sustain transmission. It also highlights the insufficiency of using a single technique for typhoid fever surveillance in healthy children living in endemic areas.
Article
Public, Environmental & Occupational Health
Nafiseh Ghorbani, Mehdi Assmar, Nour Amirmozafari, Khosrow Issazadeh
Summary: The study demonstrated the immunogenicity of a candidate vaccine against Salmonella enterica serovar Typhi adsorbed onto Bacillus subtilis spores in BALB/c mice, showing activation of the mice's humoral immune system and their ability to resist bacterial infection. The results suggest the potential use of spores as natural adjuvants for recombinant vaccines against bacterial pathogens.
IRANIAN JOURNAL OF PUBLIC HEALTH
(2021)
Article
Public, Environmental & Occupational Health
Kokab Jabeen, Sidrah Saleem, Summiya Nizamuddin, Faiqa Arshad, Shah Jahan, Faisal Hasnain, Waleed Tariq, Muhammad Junaid Tahir, Zohaib Yousaf, Muhammad Sohaib Asghar
Summary: This study aimed to evaluate the minimum inhibitory concentration (MIC) of azithromycin (AZM) in clinical isolates of extensively drug-resistant (XDR) Salmonella Typhi using the E-test versus the broth microdilution method. The study was conducted in Lahore, Pakistan and included 150 XDR Salmonella Typhi isolates. The results showed a high concordance between the E-test and broth microdilution method for determining the MIC of azithromycin.
AMERICAN JOURNAL OF TROPICAL MEDICINE AND HYGIENE
(2023)
Article
Infectious Diseases
Lovely Barai, Md Rokibul Hasan, Jalaluddin Ashraful Haq, Chowdhury Rafiqul Ahsan
Summary: Serum salmonellacidal antibody can be used to detect the functional capacity of antibody in patients with enteric fever and after typhoid vaccination. The study found that patients with typhoid and paratyphoid A had significantly higher levels of salmonellacidal antibody titers compared to the control group, and the titers increased significantly with the duration of fever. However, the titers significantly decreased after a certain period of illness. Additionally, although low salmonellacidal titers were detected after Vi CPS vaccine among the human volunteers, the mean titer increased after vaccination.
INTERNATIONAL JOURNAL OF INFECTIOUS DISEASES
(2022)
Article
Biochemistry & Molecular Biology
Aqsa Shaheen, Fouzia Ismat, Mazhar Iqbal, Abdul Haque, Zaheer Ul-Haq, Osman Mirza, Rita De Zorzi, Thomas Walz, Moazur Rahman
Summary: This study characterized a multidrug transporter styMdtM from Salmonella, showing the importance of residues Asp25 and Arg111 in substrate transport. The findings provide new insights into the structural and mechanistic aspects of the Salmonella MDR transporter styMdtM.
PROTEINS-STRUCTURE FUNCTION AND BIOINFORMATICS
(2021)
Article
Biotechnology & Applied Microbiology
Sadanand Dangari Akshay, Karanth Padyana Anupama, Vijaya Kumar Deekshit, Anusha Rohit, Biswajit Maiti
Summary: This study evaluated the expression of outer membrane proteins (OMPs) in Salmonella Typhi at sub-lethal concentrations of ceftriaxone. The results revealed the potential involvement of OMPs in the drug resistance of S. Typhi, providing insights for the development of therapeutic strategies against multi-drug resistant isolates of S. Typhi.
WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY
(2022)
Article
Immunology
Carlos Henrique Camargo, Amanda Yaeko Yamada, Andreia Rodrigues de Souza, Claudio Tavares Sacchi, Alex Domingos Reis, Marlon Benedito Nascimento Santos, Denise Brandao de Assis, Eneas de Carvalho, Elizabeth Harummyy Takagi, Marcos Paulo Vieira Cunha, Monique Ribeiro Tiba-Casas
Summary: This study reveals the silent spread of New Delhi metallo-beta-lactamase (NDM) carbapenemase in Brazil, particularly in Enterobacterales other than Klebsiella spp. It highlights the diverse species and coexistence of important resistance genes in these NDM-producing strains. Prompt detection and effective measures are crucial to mitigate the further dissemination of NDM carbapenemase and preserve antimicrobial therapy options in hospital settings.
MICROBIOLOGY AND IMMUNOLOGY
(2024)