期刊
MICROBIOLOGY AND IMMUNOLOGY
卷 52, 期 12, 页码 585-590出版社
WILEY
DOI: 10.1111/j.1348-0421.2008.00076.x
关键词
macrophage-colony-stimulating factor (M-CSF); osteoclast; RAW 264; 7 cells; receptor activator of nuclear factor-kappa B ligand (RANKL); tartrate-resistant acid phosphatase (TRAP)
资金
- Ministry of Education, Science, Sports and Culture of Japan
RAW 264.7 macrophage cells differentiate into osteoclast-like cells in the presence of RANKL. Participation of M-CSF in RANKL-induced osteoclast formation of RAW 264.7 cells was examined. TRAP-positive osteoclast-like cells appeared in RAW 264.7 cells cultured in the presence of RANKL. RANKL-induced osteoclast formation was markedly inhibited by anti-M-CSF antibody. RANKL augmented M-CSF mRNA expression and M-CSF production in RAW 264.7 cells. Further, anti-M-CSF antibody inhibited the expression of RANK, c-fms, c-fos and TRAP mRNA in RANKL-stimulated RAW 264.7 cells. However, anti-M-CSF antibody did not affect the expression of DC-STAMP in the stimulated cells. Therefore, RANKL was suggested to induce osteoclast formation in RAW 264.7 cells via augmented production of M-CSF. The putative role of M-CSF in RANKL-induced osteoclast formation of RAW 264.7 cells is discussed.
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