Article
Biotechnology & Applied Microbiology
Vijay Shankar Singh, Basant Kumar Dubey, Sushant Rai, Surendra Pratap Singh, Anil Kumar Tripathi
Summary: This study identified the genes responsible for D-glucose utilization ability in A. brasilense Sp7 by cloning and comparing genomes. The transcriptional fusion of these genes improved the D-glucose utilization ability and root colonization of rice seedlings.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2022)
Article
Soil Science
Salome Urrea-Valencia, Rafael Mazer Etto, Willian Yuiti Takahashi, Eduardo Favero Caires, Angelo Rafael Bini, Ricardo Antonio Ayub, Maria Isabel Stets, Leonardo Magalhaes Cruz, Carolina Weigert Galvao
Summary: Although Azospirillum brasilense is commonly used in inoculant formulations, its prevalence in field conditions is still limited. In this study, A. brasilense was found in soil only at radicle emergency, and from the fifth day onwards, it was detected in roots at concentrations ranging from 10(3) to 10(5) CFU g(-1) of fresh root. This research provided a sensitive method for monitoring A. brasilense in field trials and offered new insights into the ecology of maize and A. brasilense interaction.
APPLIED SOIL ECOLOGY
(2021)
Article
Biochemical Research Methods
Tim Schnabel, Elizabeth Sattely
Summary: Bioavailable nitrogen is crucial for agricultural food production, and engineered diazotrophs show potential for plant growth promotion. However, balancing ammonia production rates and fitness defects is important. Multi-copy stable strains of Azospirillum brasilense exhibit improved plant growth promotion in hydroponic conditions, but their effectiveness in nonsterile soil may be limited.
ACS SYNTHETIC BIOLOGY
(2021)
Article
Multidisciplinary Sciences
Grant Wiggins, Jacklyn Thomas, Yasir Rahmatallah, Connor Deen, Allee Haynes, Zachariah Degon, Galina Glazko, Arijit Mukherjee
Summary: In this study, we investigated the beneficial association between rice and the plant growth-promoting bacterium Herbaspirillum seropedicae. By identifying differentially expressed genes, we found that these genes play important roles in the growth and metabolism of rice, and some of them are shared with another plant growth-promoting bacterium, Azospirillum brasilense.
SCIENTIFIC REPORTS
(2022)
Article
Multidisciplinary Sciences
Koji Murakami, Tsutomu Tamada, Daisuke Saigusa, Eisaku Miyauchi, Masayuki Nara, Masakazu Ichinose, Makoto Kurano, Yutaka Yatomi, Hisatoshi Sugiura
Summary: This study found that urine ATX levels may be a novel and useful indicator for assessing disease activity in sarcoidosis.
SCIENTIFIC REPORTS
(2022)
Article
Biotechnology & Applied Microbiology
Tim Schnabel, Elizabeth Sattely
Summary: Modern agriculture requires more nitrogen than most arable soils can provide, necessitating the use of nitrogen fertilizers to sustain productivity. This study presents a metabolic engineering strategy for overproducing ammonia in Azospirillum brasilense, which can enhance plant biomass and chlorophyll content when grown in coculture with Setaria viridis. Through a rational design approach, this study demonstrates the potential for tunable ammonia production and nitrogen transfer to plant biomass, offering a sustainable solution for enhancing crop productivity.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2021)
Article
Chemistry, Analytical
Xinfa Cai, Meijuan Liang, Fei Ma, Sherif Ramzy Mohamed, Amira Abdel Goda, Dawood H. Dawood, Li Yu, Peiwu Li
Summary: A direct competitive nanozyme-linked immunosorbent assay based on MnO2 nanosheets as a nanozyme label was developed for highly sensitive determination of fumonisin B-1. The method showed a wide linear range, low detection limit, high accuracy, and good consistency with the reference method for screening FB1 in cereals.
ANALYTICAL METHODS
(2021)
Article
Microbiology
Fabricio D. Cassan, Anahi Coniglio, Edgar Amavizca, Guillermo Maroniche, Eric Cascales, Yoav Bashan, Luz E. De-Bashan
Summary: The plant-growth-promoting bacterium Azospirillum brasilense can associate with microalgae Chlorella sorokiniana and enhance their metabolic performance. The presence of T6SS in A. brasilense is crucial for promoting the production of lipids, carbohydrates, and photosynthetic pigments in the microalgae by aiding in attachment and production of IAA phytohormones. Additionally, A. brasilense T6SS exhibits antagonistic activities against various plant pathogens, suggesting potential bio-control protection for microalgae and plants.
ENVIRONMENTAL MICROBIOLOGY
(2021)
Article
Food Science & Technology
Niloy Barua, Kayla M. Clouse, Dorivar Ruiz A. Diaz, Maggie R. Wagner, Thomas G. Platt, Ryan R. Hansen
Summary: The research describes a new method for screening and identifying bacterial consortia that promote the growth of corn. This method utilizes a microfabricated high-throughput screening device, called the microwell recovery array (MRA), to explore the maize rhizobiome for combinations of bacteria that enhance the growth and colonization of the nitrogen-fixing PGPB, Azospirillum brasilense. The study found that bacterial consortia dominated by bacteria from the Acinetobacter-Enterobacter-Serratia genera significantly accelerated the growth of corn plants when co-inoculated with A. brasilense. This research demonstrates a rapid bioprospecting method for identifying complementary PGPB and developing next-generation biofertilizers.
FRONTIERS IN SUSTAINABLE FOOD SYSTEMS
(2023)
Article
Nanoscience & Nanotechnology
Wenhao Li, Tiange Gao, Chenxi Lou, Haifang Wang, Yuanfang Liu, Aoneng Cao
Summary: This study presents a novel artificial antibody called Goldbody, which is based on gold nanoparticles and exhibits comparable specificity and improved stability compared to monoclonal antibodies. The Goldbody was successfully utilized in competitive LFIA and ELISA assays for the detection of lysozyme, demonstrating its potential as a substitute for monoclonal antibodies in immunoassays.
ACS APPLIED NANO MATERIALS
(2022)
Article
Chemistry, Analytical
Xue Liu, Xiuzhai Chen, Xu Zhu, Qing Lin, Xi Pan, Xiaolei Tan, Yongfeng Guo, Jun Qiu, Song Fang
Summary: In this study, ELISA and TRFIA methods were established for the detection and monitoring of quinclorac residue in the environment. The results of both methods were consistent with those of the referenced UPLC-MS/MS method, and they showed good detection performance that met the requirements of quinclorac quantitative determination.
Article
Chemistry, Analytical
Fatema Kaladari, Mahmoud El-Maghrabey, Naoya Kishikawa, Naotaka Kuroda
Summary: A sensitive and stable signal multiplied quinone-linked immunosorbent assay (Multi-QuLISA) was developed using a compound called Bio8mer-NQ as a signal-generating label. This compound showed better stability than the commonly used enzyme, biotin-HRP, and demonstrated higher sensitivity in both colorimetric and chemiluminescence assays.
Article
Chemistry, Analytical
Mahmoud El-Maghrabey, Yudai Sato, Fatema Kaladari, Naoya Kishikawa, Naotaka Kuroda
Summary: Enzyme-labeled immunoassays are widely used for trace analysis of clinically significant compounds. However, utilizing enzymes for labeling antibodies can lead to problems such as low reproducibility and inhibition of antigen-antibody reaction. In this study, we used quinones as stable non-enzymatic tags and developed a simple, stable, sensitive, and cost-effective approach for labeling immunoassays. We successfully developed a quinone-linked immunosorbent assay (QuLISA) for detecting food allergens, which showed good linearity and high sensitivity.
SENSORS AND ACTUATORS B-CHEMICAL
(2022)
Article
Multidisciplinary Sciences
Asrul Abdul Wahab
Summary: Anti-nuclear antibody (ANA) is a type of autoantibody that occurs before the development of autoimmune diseases. It is commonly used as a lab test to detect underlying autoimmune diseases in patients. Different laboratory methods have shown varying sensitivity and specificity in detecting ANA, and its interpretation is complex as it can be present in non-autoimmune conditions and healthy individuals. Recent advances in ANA testing aim to harmonize reporting and include the establishment of International Consensus of ANA Pattern (ICAP) and automated ANA IF reader.
Article
Food Science & Technology
Shuangmin Wu, Huaming Li, Xiaoyang Yin, Yu Si, Liangni Qin, Hongfei Yang, Jiaxu Xiao, Dapeng Peng
Summary: An indirect competitive enzyme-linked immunoassay (ic-ELISA) was established to detect PYR and BaP residues in living aquatic products for the first time, with high sensitivity and accuracy.
Article
Microbiology
Irina V. Yegorenkova, Kristina V. Tregubova, Alexander I. Krasov, Nina V. Evseeva, Larisa Yu. Matora
Summary: Paenibacillus polymyxa is a promising plant-growth-promoting rhizobacterium that can increase the mitotic index of root cells and promote growth in wheat seedlings. In addition, this bacterium can intensely colonize the root surface, showing potential for developing effective biofertilizers for agricultural purposes.
JOURNAL OF MICROBIOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Oksana V. Tkachenko, Gennady L. Burygin, Nina V. Evseeva, Yulia P. Fedonenko, Larisa Yu. Matora, Yuriy V. Lobachev, Sergei Yu. Shchyogolev
Summary: Callus tissue is an important tool in modern plant breeding and biotechnology. The lipopolysaccharides (LPSs) of Azospirillum strains had different effects on wheat calluses, with A. lipoferum SR65 showing the most significant promotion of callus morphogenesis. The LPSs of some Azospirillum strains may be promising promoters of plant morphogenesis in the future.
PLANT CELL TISSUE AND ORGAN CULTURE
(2021)
Article
Agriculture, Multidisciplinary
K. Yu Kargapolova, O. Tkachenko, G. Burygin, N. Evseeva, A. A. Shirokov, L. Yu Matora, S. Yu Shchyogolev
Summary: This study examined the effects of inoculation with plant-growth-promoting rhizobacteria on the growth and yield of potato microplants. In vitro inoculation promoted root formation and growth in microplants and also showed positive effects in greenhouse cultivation. Simultaneous inoculation not only improved the survival ability of microplants in the greenhouse, but also increased the yield of minitubers.
VAVILOVSKII ZHURNAL GENETIKI I SELEKTSII
(2022)
Article
Biochemistry & Molecular Biology
Natalia A. Shushunova, Oksana A. Mayorova, Ekaterina S. Prikhozhdenko, Olga A. Goryacheva, Oleg A. Kulikov, Valentina O. Plastun, Olga I. Gusliakova, Albert R. Muslimov, Olga A. Inozemtseva, Nikolay A. Pyataev, Alexander A. Shirokov, Dmitry A. Gorin, Gleb B. Sukhorukov, Olga A. Sindeeva
Summary: Complex immunosuppressive therapy is used to treat glomerulonephritis, but it has severe side effects. This study demonstrates the effectiveness of polymer capsules containing etanercept in the kidneys of mice. Intra-arterial administration of the capsules showed significant superiority over injection into the tail vein. The proposed strategy of endovascular surgery combined with polymer microcapsules can be applied to treat kidney diseases associated with glomerular pathology.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Microbiology
D. I. Mokeev, I. V. Volokhina, E. M. Telesheva, S. S. Evstigneeva, V. S. Grinev, T. E. Pylaev, L. P. Petrova, A. V. Sheludko
Summary: Polyethylene glycol (PEG 6000) was used to establish osmotic stress conditions for Azospirillum brasilense Sp7 and its variants. The study found that PEG 6000-caused osmotic stress promoted biofilm formation in Sp7.8. Sp7.4 variant was the most sensitive to water stress in terms of biofilm formation, but showed higher growth variables in planktonic culture under stress compared to Sp7 and Sp7.8.
Article
Microbiology
L. P. Petrova, S. S. Evstigneeva, Y. A. Filip'echeva, I. V. Volokhina, G. L. Burygin, L. Y. Matora, A. V. Shelud'ko
Summary: Inactivation of gene CDS AZOBR_p60123 of the ABC transporter ATP-binding protein Wzt in the p60123::aphAI mutant of Azospirillum baldaniorum Sp245, and introduction of an additional copy of this gene into the parent or mutant strain, affected the heterogeneity, immunochemical characteristics, and strain properties related to polysaccharide production (biofilm construction) of O polysaccharides (OPSs) and lipopolysaccharides (LPSs). Biofilms of the p60123::aphAI mutant Sp245.4-1-1 accumulated two times less biomass than wild-type Sp245. Introducing pRK415-p60123 into Sp245.4-1-1 or Sp245 cells resulted in an increase or decrease in biofilm biomass accumulation ability of the derived strains compared to the original strains.
Article
Chemistry, Physical
Nikita Navolokin, Maria Lomova, Alla Bucharskaya, Olga Godage, Natalya Polukonova, Alexander Shirokov, Vyacheslav Grinev, Galina Maslyakova
Summary: The study examined the effects of encapsulated and native form of Gratiola officinalis extract on breast carcinoma and human cervical cancer cells. The encapsulated extract demonstrated significant cell death in both cancer cell types and prevented the formation of autophagosomes. Microencapsulation could be considered as an effective strategy for delivering Gratiola officinalis extract to tumor cells and overcoming protective autophagy.
Article
Pharmacology & Pharmacy
Oxana Semyachkina-Glushkovskaya, Denis Bragin, Olga Bragina, Sergey Socolovski, Alexander Shirokov, Ivan Fedosov, Vasily Ageev, Inna Blokhina, Alexander Dubrovsky, Valeria Telnova, Andrey Terskov, Alexander Khorovodov, Daria Elovenko, Arina Evsukova, Maria Zhoy, Ilana Agranovich, Elena Vodovozova, Anna Alekseeva, Juergen Kurths, Edik Rafailov
Summary: Low-level laser treatment (LLLT) can open the blood-brain barrier (BBB) and improve drug delivery to the brain, which is significant for the treatment of brain diseases.
Article
Biochemistry & Molecular Biology
Oxana Semyachkina-Glushkovskaya, Sergey Sokolovski, Ivan Fedosov, Alexander Shirokov, Nikita Navolokin, Alla Bucharskaya, Inna Blokhina, Andrey Terskov, Alexander Dubrovski, Valeria Telnova, Anna Tzven, Maria Tzoy, Arina Evsukova, Daria Zhlatogosrkaya, Viktoria Adushkina, Alexander Dmitrenko, Maria Manzhaeva, Valeria Krupnova, Alessio Noghero, Denis Bragin, Olga Bragina, Ekaterina Borisova, Juergen Kurths, Edik Rafailov
Summary: This study demonstrates the efficacy of photosensitizer-free laser treatment using a quantum-dot-based 1267 nm laser diode in suppressing the growth of glioblastoma (GBM) in rats. The findings suggest that this technology has the potential to be a breakthrough in non-invasive or minimally invasive therapy for superficial GBMs, particularly in infants and patients with high photosensitivity or allergies to photosensitizers.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biotechnology & Applied Microbiology
Andrei Shelud'ko, Irina Volokhina, Dmitry Mokeev, Elizaveta Telesheva, Stella Yevstigneeva, Andrei Burov, Anna Tugarova, Alexander Shirokov, Gennady Burigin, Larisa Matora, Lilia Petrova
Summary: This study investigated the two-component signal transduction system (TCS) in A. baldaniorum bacteria, which regulates their response to mechanical stress and osmotic/water stress. The mutant showed changes in bacterial morphology, motility, and secretion apparatus. Under osmotic stress, the mutant lost its collective motility.
WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY
(2023)
Article
Multidisciplinary Sciences
Dongyu Li, Shaojun Liu, Tingting Yu, Zhang Liu, Silin Sun, Denis Bragin, Alexander Shirokov, Nikita Navolokin, Olga Bragina, Zhengwu Hu, Juergen Kurths, Ivan Fedosov, Inna Blokhina, Alexander Dubrovski, Alexander Khorovodov, Andrey Terskov, Maria Tzoy, Oxana Semyachkina-Glushkovskaya, Dan Zhu
Summary: This study identifies a pathological mechanism of removing blood from the brain after intraventricular hemorrhage through transcranial near-infrared phototherapy and provides a method for fast recovery and improvement of behavioral outcomes.
NATURE COMMUNICATIONS
(2023)
Article
Biochemistry & Molecular Biology
S. S. Evstigneeva, D. Mokeev, L. P. Petrova, A. Shelud'ko
Summary: The aim of this study was to investigate the involvement of the HSHK-RR and FlhB proteins in mechanosensing and mechanotransduction in Azospirillum baldaniorum cells. The results demonstrated that mutations in the FlhB gene or an increase in the copy number of the HSHK-RR gene affected cell motility, ultrastructure, and dynamics of cell size changes. It was also found that these proteins are involved in the perception and transmission of mechanical signals in response to changes in the mechanical properties of the medium.
MOLECULAR GENETICS MICROBIOLOGY AND VIROLOGY
(2022)
