Article
Biochemistry & Molecular Biology
Nor E. L. Houda Tahiri, Hamza Saghrouchni, Noureddine Hamamouch, Mostafa El Khomsi, Abdulhakeem Alzahrani, Ahmad Mohammad Salamatullah, Lyoussi Badiaa, Lalla Aicha Lrhorfi
Summary: This study evaluated the sensitivity of fungal pathogens of citrus fruit to various pesticides and 1,8-cineole, and found that 1,8-cineole may be recommended as an alternative to conventional fungicides. However, caution should be taken when using glyphosate in citrus fields.
Article
Agronomy
Wenjun Wang, Ting Li, Jialin Chen, Xuan Zhang, Linli Wei, Shixiang Yao, Kaifang Zeng
Summary: Green mold caused by Penicillium digitatum is a major postharvest disease in citrus fruit, but the molecular mechanism related to the induced disease resistance in citrus is still unclear. This study investigated the regulatory function of CsWRKY33 on citrus disease resistance. The results showed that CsWRKY33 played an important role in regulating disease resistance pathways and activating the expression of genes involved in disease resistance.
POSTHARVEST BIOLOGY AND TECHNOLOGY
(2023)
Article
Agronomy
Jialin Chen, Wenjun Wang, Ting Li, Xiaoquan Gao, Xuan Zhang, Kaifang Zeng
Summary: This study discovered that CsAP2L can enhance the resistance to green mold in citrus fruit through transient overexpression. Additionally, it was found that CsAP2L can activate the expression of disease resistance genes and repress the transcription of other genes, thus improving fruit resistance. This study provides new insights into the mechanism of fruit disease resistance.
POSTHARVEST BIOLOGY AND TECHNOLOGY
(2023)
Article
Cell Biology
Alessandra da Silva Dantas, Filomena Nogueira, Keunsook K. Lee, Louise A. Walker, Matt Edmondson, Alexandra C. Brand, Megan D. Lenardon, Neil A. R. Gow
Summary: The study demonstrates that simultaneous activation of the calcineurin and cell wall integrity (CWI) pathways leads to the generation of non-viable Candida albicans yeast cells with supra-normal levels of chitin in the cell wall, disrupting cell expansion and growth. Mutations in the Ca2+-calcineurin pathway prevent the formation of these non-viable cells by negatively regulating chitin synthesis driven by the CWI pathway. The Ca2+-calcineurin pathway may serve as an attenuator to prevent overproduction of chitin by coordinating chitin upregulation and negative regulation of the CWI signaling pathway.
JOURNAL OF CELL SCIENCE
(2021)
Article
Microbiology
Marta de Ramon-Carbonell, Paloma Sanchez-Torres
Summary: Zn(2)Cys(6) transcription factors unique to fungi play different regulatory functions. The deletion of the PdMut3 gene in Penicillium digitatum resulted in increased virulence during citrus infection, higher expression rate during fungal growth, and less transcription during fruit infection. Additionally, mutants of this gene showed growth reduction, altered development of conidiophores, increased sensitivity to compounds affecting the cell wall, and an invasive growth block. The findings suggest that PdMut3 may indirectly contribute to fungal virulence through metabolism and peroxisomes development.
Article
Chemistry, Applied
Wenjun Wang, Ting Li, Qi Chen, Shixiang Yao, Kaifang Zeng
Summary: As the most productive fruit in the world, there is a lack of research on disease resistance mechanisms in citrus. This study identified CsWRKY23 as a mediator of disease resistance in citrus, enhancing resistance to Penicillium digitatum. Through DNA affinity purification sequencing and RNA sequencing, 48 direct target genes of CsWRKY23 were identified. CsWRKY23 contributed to SA synthesis pathway, ROS accumulation, and cell wall strengthening, forming a possible regulatory network for disease resistance.
Article
Microbiology
Xiujun Luo, Xiaori Zhan, Ruoxin Ruan, Yue Xi, Chenjia Shen, Huizhong Wang, Mingshuang Wang
Summary: This study systematically identified and analyzed the bZIP family in 23 Penicillium species, revealing the impact of gene loss and gene duplication on the evolution of the Penicillium bZIP family. The study also characterized the biological functions of an important member, PdatfA, in P. digitatum, showing its effects on growth, pigmentation, and resistance.
RESEARCH IN MICROBIOLOGY
(2022)
Article
Genetics & Heredity
Kaili Zhao, Zhongjiao Liu, Mengxue Li, Yueyan Hu, Ling Yang, Xin Song, Yuqi Qin
Summary: Fungi sense environmental signals and coordinate growth, development, and metabolism accordingly. The calcineurin-CrzA pathway in Penicillium oxalicum plays a crucial role in regulating cellular processes and gene expression, impacting growth and metabolism.
FUNGAL GENETICS AND BIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Dengxian Xi, Tuo Yin, Peichen Han, Xiuyao Yang, Mengjie Zhang, Chaojin Du, Hanyao Zhang, Xiaozhen Liu
Summary: In this study, CsWRKY transcription factors were characterized in sweet orange in response to P. digitatum infection. Genome-wide identification, classification, phylogenetic and conserved domain analysis were performed. Transcriptome data revealed the expression pattern of each CsWRKY gene under P. digitatum stress and predicted their functions. The results showed that CsWRKY genes played a crucial role in transcriptional regulation and defense against pathogens in sweet orange, with CsWRKY2 and CsWRKY14 being potential candidate genes for genetic improvement.
CURRENT ISSUES IN MOLECULAR BIOLOGY
(2023)
Article
Genetics & Heredity
Guanggan Hu, Linda Horianopoulos, Eddy Sanchez-Leon, Melissa Caza, Wonhee Jung, James W. Kronstad
Summary: Monothiol glutaredoxins, including Grx4, are crucial factors in regulating iron homeostasis and play essential roles in cell proliferation, cell wall integrity, and stress responses at elevated temperatures in Cryptococcus neoformans.
G3-GENES GENOMES GENETICS
(2021)
Proceedings Paper
Agronomy
K. Ncama, L. S. Magwaza, A. Mditshwa, S. Z. Tesfay, N. C. Mbili
Summary: Application of chemical fungicides and waxes on fresh horticultural produce has drawbacks, whereas organic antifungal agent such as moringa leaf extract extracted using water or ethanol could offer effective and cost-efficient solutions.
II INTERNATIONAL SYMPOSIUM ON MORINGA
(2021)
Article
Multidisciplinary Sciences
Kihyoun Park, Hyejin Lim, Jinyoung Kim, Yeseong Hwang, Yu Seol Lee, Soo Han Bae, Hyeongseok Kim, Hail Kim, Shin-Wook Kang, Joo Young Kim, Myung-Shik Lee
Summary: This study reveals the crucial role of lysosomal calcium release in promoting mitophagy and maintaining pancreatic beta-cell function. The activation of transcription factor EB (TFEB) through lysosomal calcium release leads to increased mitophagy, while the loss of beta-cell TFEB aggravates glucose intolerance during high-fat diet.
NATURE COMMUNICATIONS
(2022)
Article
Microbiology
Ana Belen Sanz, Sonia Diez-Muniz, Jennifer Moya, Yuliya Petryk, Cesar Nombela, Jose M. Rodriguez-Pena, Javier Arroyo
Summary: Conditions altering the yeast cell wall lead to the activation of an adaptive transcriptional response mainly governed by the cell wall integrity (CWI) mitogen-activated protein kinase (MAPK) pathway. Two high-throughput screenings were conducted to identify genes related to cell wall integrity and transcriptional program elicited by cell wall stress. Functional groups related to chromatin organization, Golgi vesicle transport, rRNA processing, and protein glycosylation were found to be highly representative. Additionally, genes associated with RNA metabolism, transcription/translation, protein degradation, and chromatin organization were highlighted.
Article
Biochemistry & Molecular Biology
Austin Mottola, Bernardo Ramirez-Zavala, Kerstin Huenniger, Oliver Kurzai, Joachim Morschhaeuser
Summary: The fungal cell wall is crucial for cellular integrity and environmental interactions. A network of signaling pathways in Candida albicans regulates cell wall structure, with hyperactive Czf1 inducing the expression of cell wall-related genes and causing visible changes in cell wall architecture. Manipulation of Czf1 activity can influence the sensitivity of C. albicans to cell wall stress and antifungal drugs.
MOLECULAR MICROBIOLOGY
(2021)
Article
Microbiology
Lingdan Lian, Guang Zhang, Jing Zhu, Yunxiao Wang, Lingshuai Wang, Rui Liu, Liang Shi, Ang Ren, Mingwen Zhao
Summary: This study identified two different transcripts of Swi6, with the isoform GlSwi6B participating in regulating the cell wall integrity signaling in Ganoderma lucidum fungi. GlSwi6B was found to bind to the promoters of chitin and glucan synthesis genes, while this binding phenomenon was not observed in the isoform GlSwi6A.
ENVIRONMENTAL MICROBIOLOGY
(2021)
Article
Dermatology
Jiaxing You, Chao Liu, Yixin Chen, Weifen Zhu, Hongye Li, Lin Li
Summary: The aim of this study was to identify the most cited articles in the field of diabetic foot ulcer (DFU) research. The study conducted a bibliometric analysis using Boolean search terms in two databases. The findings revealed the characteristics of the top-cited articles, such as publication year, country of origin, and level of evidence. The majority of the articles were clinical studies, providing valuable insights into the history and development of DFU research.
INTERNATIONAL JOURNAL OF LOWER EXTREMITY WOUNDS
(2023)
Article
Chemistry, Medicinal
Yang Liu, Siyuan Xu, Qiudie Cai, Dawei Li, Hongye Li, Weidong Yang
Summary: In this study, we investigated the interaction between okadaic acid (OA) and gut microbiota using an in vitro fermentation experiment. We found that OA can reshape the bacterial composition and lead to the production of various metabolites. Our findings confirmed the direct interaction between OA and gut bacteria, providing valuable evidence for understanding the metabolic process and complex toxicity of OA.
Article
Engineering, Environmental
Yuhan Ma, Wasiqi Yuan, Wenjie Kan, Changyue Huang, Jiawei Zhu, Guilong Zhang, Hongye Li, Srinivasan Balamurugan, Lifang Wu
Summary: Microalgae are being investigated as green cell factories for biochemistry and pollution control. This study focuses on enhancing the synthesis of selenium nanoparticles (SeNPs) in microalgae and understanding the molecular mechanisms involved. Chlorella vulgaris was fortified with quercetin to promote SeNPs biotransformation. Quercetin facilitated the formation of SeNPs within 72 hours, converting almost 97% of inorganic selenite to SeNPs. Electron microscopy confirmed the biogenic ability of the algal system. Quercetin fortification protected C. vulgaris from selenite-induced impairment and improved its growth rate, while also altering the fatty acid profile.
CHEMICAL ENGINEERING JOURNAL
(2023)
Article
Biochemistry & Molecular Biology
Chaofan Zhang, Chen Jiao, Xuepeng Sun, Xiaolong Li
Summary: This study conducted a comprehensive analysis of the MYB superfamily in 437 different plant species to explore its evolutionary contributions to species diversification, trait formation, and environmental adaptation. The research revealed major changes in gene copy number and genomic context within subclades and proposed the role of transposable element insertion in promoting sub-/neo-functionalization of MYB genes. It emphasizes the importance of lineage-/tissue-specific characterization to understand trait variability and environmental adaptation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Plant Sciences
Xiao-Li Hu, Congjun You, Kaikai Zhu, Xiaolong Li, Jinli Gong, Haijie Ma, Xuepeng Sun
Summary: The number of studies on plant transcriptomes using ONT RNAseq technology is rapidly increasing. It is a powerful method for deciphering transcriptomic complexity, especially for alternative splicing event detection. Exploring different alternative splicing events in citrus plants contributes to improving transcriptomes and studying functional genomes.
FRONTIERS IN PLANT SCIENCE
(2023)
Article
Agronomy
Bei Liu, Xingxing Liang, Jinchao Kong, Chen Jiao, Hongye Li, Yunpeng Gai
Summary: In this study, the genetic structure and diversity of Colletotrichum gloeosporioides, the dominant species causing citrus anthracnose, were analyzed using the Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) gene. A total of 19 GAPDH haplotypes were identified, with the main haplotype (haplotype 5) distributed widely among isolates from different Citrus species and locations. The genetic differentiation index (Fst) revealed significant genetic differentiation between populations from different regions and host species. The results suggest that genetic differentiation in C. gloeosporioides is mainly influenced by gene mutation, recombination, or migration within native populations rather than natural selection caused by geography or host variety.
Article
Agriculture, Multidisciplinary
Li-Gong Zou, Yi-Tong Yao, Fu-Fang Wen, Xiao Zhang, Bo-Tao Liu, Da-Wei Li, Yu-Feng Yang, Wei-Dong Yang, Srinivasan Balamurugan, Hong-Ye Li
Summary: The excessive accumulation of catechol in the environment due to its burgeoning commercial applications poses a severe ecological threat. This study investigated the potential of the microalga Crypthecodinium cohnii to degrade catechol and utilize the byproduct as a carbon source. Results showed that catechol significantly increased the growth of C. cohnii and was rapidly catabolized within 60 hours. Transcriptomic analysis identified key genes involved in catechol degradation, and real-time polymerase chain reaction (RT-PCR) analysis revealed a remarkable increase in the transcription of these genes. The study also demonstrated altered primary metabolite content and the ability of C. cohnii to tolerate catechol treatment without adverse effects.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2023)
Article
Green & Sustainable Science & Technology
Zi-Hao Qin, Xiaomeng Hu, Jin-Hua Mou, Guo-Hui He, Guang-Bin Ye, Hong-Ye Li, Shauhrat Singh Chopra, Liang Dong, Carol Sze Ki Lin, Xiang Wang
Summary: This study conducted Life Cycle Assessments (LCA) to evaluate the environmental impact of laboratory-scale EPA production by Phaeodactylum tricornutum. The results showed that technical advancements, particularly metabolic engineering, significantly reduced the global warming potential and cumulative energy demand associated with EPA production. Electricity consumption and extraction solvents were identified as the main contributors to the environmental impact. The study suggests future directions should focus on increasing EPA productivity, adopting alternative cultivation approaches with less energy input, and implementing environmentally friendly methods for EPA recovery.
JOURNAL OF CLEANER PRODUCTION
(2023)
Article
Chemistry, Medicinal
Kuan-Kuan Yuan, Zi-Min Chen, Ya-Xin Liu, Hong-Ye Li, Wei-Dong Yang
Summary: This study found that docosahexaenoic acid (DHA) can enhance the response of Perna viridis to diarrhetic shellfish toxins (DSTs) produced by Prorocentrum lima. The addition of DHA increased the esterification level of DSTs, activated the Nrf2 signaling pathway, and alleviated the damage to the digestive glands. These findings provide insights into the role of DHA in the environmental adaptation of bivalves.
Article
Multidisciplinary Sciences
Heqiang Lou, Lili Song, Xiaolong Li, Hailing Zi, Weijie Chen, Yadi Gao, Shan Zheng, Zhangjun Fei, Xuepeng Sun, Jiasheng Wu
Summary: The authors assembled the genome of Torreya grandis, a gymnosperm species with edible seeds containing sciadonic acid. They identified two key genes involved in sciadonic acid biosynthesis and found that seed development is accompanied by DNA methylation changes. This study provides important genomic resources and insights into the evolutionary mechanism of sciadonic acid biosynthesis in land plants.
NATURE COMMUNICATIONS
(2023)
Article
Agronomy
Yunpeng Gai, Qichen Niu, Jinchao Kong, Lei Li, Xingxing Liang, Yuwei Cao, Xianqi Zhou, Xuepeng Sun, Haijie Ma, Mingshuang Wang, Neeraj Shrivastava, Hongye Li, Chen Jiao
Summary: A comparative transcriptome analysis was performed to study the gene expression patterns of Alternaria alternata during infection of citrus. Significant gene expression differences were found between the early and late stages of infection. Several novel genes related to plant-pathogen interaction were identified. RT-qPCR analysis confirmed the expression patterns of selected genes from the peroxisome pathway.
Article
Microbiology
Wen Wang, Tao Xiong, Yating Zeng, Wenwen Li, Chen Jiao, Jianping Xu, Hongye Li
Summary: This study conducted an extensive survey of Phyllosticta species across 11 citrus-producing provinces in southern China and identified five Phyllosticta species with pathogenicity on different citrus species. The findings have important implications for the control and management of Citrus Black Spot and related diseases.
Article
Engineering, Environmental
Jin-Hua Mou, Zi-Hao Qin, Yu-Feng Yang, Si-Fen Liu, Wei Yan, Lan Zheng, Ya-Hui Miao, Hong-Ye Li, Patrick Fickers, Carol Sze Ki Lin, Xiang Wang
Summary: This study investigated the characteristics of four types of food waste and their hydrolysates, and explored their potential application in biorefineries. The results showed that the characteristics of food waste and their hydrolysates varied based on their origins, and the origin and storage conditions of food waste affected microalgal growth and paramylon production.
CHEMICAL ENGINEERING JOURNAL
(2023)
Article
Marine & Freshwater Biology
Jian-Wei Zheng, Guo-Hui He, Rui-Xia Xu, Xiang Wang, Hong-Ye Li, Wei-Dong Yang
Summary: Studies have found that there are reciprocal inhibitory effects between Karenia mikimotoi and Prorocentrum shikokuense under co-cultures. RNA sequencing analysis revealed that genes involved in photosynthesis, carbon fixation, energy metabolism, nutrients absorption and assimilation were up-regulated in K. mikimotoi after co-cultured with P. shikokuense, while genes involved in DNA replication and cell cycle were down-regulated. In contrast, genes involved in energy metabolism, cell cycle, and nutrients uptake and assimilation were down-regulated in P. shikokuense under co-culture with K. mikimotoi. Additionally, the expression of PLA2G12 and nitrate reductase, which may play important roles in the allelopathy of K. mikimotoi, was significantly increased. These findings provide new insights into the interspecies competition between K. mikimotoi and P. shikokuense and offer a novel strategy for studying interspecific competition in complex systems.
Article
Biochemistry & Molecular Biology
Haijie Ma, Ningge Liu, Xuepeng Sun, Mengling Zhu, Tingfeng Mao, Suya Huang, Xinyue Meng, Hangfei Li, Min Wang, Huiling Liang
Summary: In this study, a highly efficient, labor-saving, and cost-effective genetic transformation system using Agrobacterium rhizogenes-mediated hairy roots was established for tea plants. This system bypassed tissue culture and antibiotic screening, completing the transformation process in just two months. The system was successfully used to analyze the function of transcription factor CsMYB73 and induced callus formation for further biological studies. It was also effective for multiple tea plant varieties and other woody plant species. Overcoming technical obstacles, this genetic transformation system will be valuable for routine gene investigation and precise breeding in tea plants.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Microbiology
Vera Analise Schommer, Mateus Torres Nazari, Flavia Melara, Julia Catiane Arenhart Braun, Alan Rempel, Lara Franco dos Santos, Valdecir Ferrari, Luciane Maria Colla, Aline Dettmer, Jeferson Steffanello Piccin
Summary: This review introduces the techniques and mechanisms of bacteria immobilization on biochar, as well as its applications in bioremediation and agriculture. The immobilization characteristics of biochar depend on pyrolysis methods, raw materials, and properties of biochar. Scanning electron microscope and colony forming unit analysis are commonly used to verify the immobilization efficiency. Applying biochar-immobilized bacteria can improve soil quality, plant growth, and crop yield.
MICROBIOLOGICAL RESEARCH
(2024)
Article
Microbiology
Manoj Kumar Solanki, Naveen Chandra Joshi, Prashant Kumar Singh, Sandeep Kumar Singh, Gustavo Santoyo, Lucas Carvalho Basilio de Azevedo, Ajay Kumar
Summary: The rhizosphere microbiome plays a crucial role in plant growth and health. Rhizosphere engineering can be used to modify the microbiome and improve plant productivity and resilience.
MICROBIOLOGICAL RESEARCH
(2024)
Article
Microbiology
Xin Cao, Xing-Wang Cheng, Yin-Ying Liu, Hong-Wei Dai, Ren-You Gan
Summary: Maintaining oral health is crucial for overall well-being. Imbalances in oral microorganisms can lead to various infectious diseases. Exploring alternatives for prevention and management of oral infections is important due to limitations of current treatments. Natural products offer potential as reliable, effective, and affordable therapeutic options for oral infectious diseases.
MICROBIOLOGICAL RESEARCH
(2024)
Article
Microbiology
Jose David Flores-Felix, Ana Carolina Goncalves, Sara Meirinho, Ana Raquel Nunes, Gilberto Alves, Cristina Garcia-Viguera, Diego A. Moreno, Luis R. Silva
Summary: The application of bacterial biofortifiers is increasing and has been shown to improve the nutritional characteristics of blueberries in Portugal.
MICROBIOLOGICAL RESEARCH
(2024)
Review
Microbiology
Asma Sani, Wan-Qi Qin, Jia-Yi Li, Yi-Fan Liu, Lei Zhou, Shi-Zhong Yang, Bo-Zhong Mu
Summary: This article summarizes the structural diversity of microbial lipopeptide biosurfactants and their applications as biocontrol agents in plants. Lipopeptides have low toxicity and wide range of applications, making them important in the pesticide industry.
MICROBIOLOGICAL RESEARCH
(2024)
Article
Microbiology
Xiao-Yu Sun, Jie Deng, Chenhui Zhang, Sin-Yee Fung, Kam-Leung Siu, Ying-Ying Cheng, Liumei Ye, Jiaoxia Qin, Ke Wang, Jiu-Xin Qu, Wenying Gao, Fuxiang Wang, Dong-Yan Jin, Liang Yang
Summary: In this study, a novel c-di-GMP binding protein SodA that regulates oxidative stress tolerance in S. maltophilia was identified. The enzyme activity of SodA was shown to be directly modulated by c-di-GMP through various in vivo and in vitro experiments.
MICROBIOLOGICAL RESEARCH
(2024)
