期刊
MICROBIOLOGICAL RESEARCH
卷 168, 期 9, 页码 547-554出版社
ELSEVIER GMBH
DOI: 10.1016/j.micres.2013.04.011
关键词
Quorum sensing; Aquaculture; Quorum quenching; Marine bacteria
类别
资金
- Spanish Ministerio de Educacion y Ciencia [CGL2008-02399/BOS, AGL2009-07656]
- Consejeria de Educacion Ciencia y Empresa, of the Andalucian Regional Government [P07-CVI-03150]
- Plan Andaluz de Investigacion
Quorum sensing (QS) systems, which depend on N-acylhomoserine lactone (AHL) signal molecules, mediate the production of virulence factors in many pathogenic microorganisms. One hundred and forty-six bacterial strains, isolated from a bivalve hatchery, were screened for their capacity to degrade five synthetic AHLs [N-butyryl-DL-homoserine lactone (C-4-HSL), N-hexanoyl-DL-homoserine lactone (C-6-HSL), N-octanoyl-DL-homoserine lactone (C-8-HSL), N-decanoyl-DL-homoserine lactone (C-10-HSL) and N-dodecanoyl-DL-homoserine lactone (C-12-HSL)] using well diffusion agar-plate assays with three biosensors, Chromobacterium violaceum CV026, C. violaceum VIR07 and Agrobacterium tumefaciens NTL4 (pZLR4). The results of these assays led to our choosing four strains (PP2-67, PP2-459, PP2-644 and PP2-663) that were able to degrade all five synthetic AHLs, thus showing a wide spectrum of quorum quenching (QQ) activity. We subsequently confirmed and measured the QQ activity of the four strains by high-performance liquid chromatography plus mass-spectrometry analysis (HPLC-MS). One of the strains which showed the highest AHL-degrading activity, PP2-459, identified as being a member of the genus Thalassomonas was chosen for further study. Finally, using thin-layer chromatography (TLC), we went on to confirm this strain's capacity to degrade the AHLs produced by other non-pathogenic and pathogenic bacteria not taxonomically related. (c) 2013 Elsevier GmbH. All rights reserved.
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