期刊
MICROBIOLOGICAL RESEARCH
卷 167, 期 9, 页码 532-535出版社
ELSEVIER GMBH, URBAN & FISCHER VERLAG
DOI: 10.1016/j.micres.2012.02.010
关键词
Global regulators; Phosphate control; GlnR; PhoR-PhoP; AfsR
类别
资金
- grant CICYT (Ministry of Science and Innovation, Madrid)
- SYSMO STREAM Project of the European Union [GEN2006-27745-E/SYS]
- Junta de Castilla y Leon Project [GR.119]
- [Bio2006-14853-O2-1]
- [BIO2010-16094]
Growth of soil bacteria is often limited by the availability of essential nutrients such as carbon, nitrogen and phosphate. The reaction to a specific nutrient starvation triggers interconnected responses to equilibrate the metabolism. It is known that PhoP (response regulator involved in phosphate control) specifically binds to several promoters of genes involved in nitrogen metabolism which are also regulated by GlnR (regulator involved in nitrogen control). In this article we report a novel cross-talk between GlnR and the SARP-like regulator, AfsR. AfsR binds to some PhoP-regulated promoters including those of afsS (a small regulatory protein of secondary metabolism), pstS (a component of the phosphate transport system) and phoRP (encoding the two component system itself). We have characterized the regulation exerted upon the nitrogen regulator glnR gene by AfsR, using EMSA and DNase I footprinting assays as well as in vivo expression studies with Delta phoP, Delta afsR and Delta afsR-Delta phoP mutants. Both PhoP and AfsR proteins are able to bind to overlapping regions within the glnR promoter producing different effects. This work demonstrates a cross-talk of three different regulators of both primary and secondary metabolism. (C) 2012 Elsevier GmbH. All rights reserved.
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