期刊
MICROBIOLOGICAL RESEARCH
卷 167, 期 7, 页码 388-394出版社
ELSEVIER GMBH
DOI: 10.1016/j.micres.2012.01.003
关键词
Pseudomonas brassicacearum; 2,4-DAPG; Biocontrol; phlACBD gene cluster; Ralstonia solanacearum
类别
资金
- China Agriculture Ministry [201103004]
- National Nature Science Foundation of China [40871126]
- Chinese Ministry of Science and Technology [2010AA10Z401]
A bacterial strain, J12, isolated from the rhizosphere soil of tomato plants strongly inhibited the growth of phytopathogenic bacteria Ralstonia solanacearum. Strain J12 was identified as Pseudomonas brassicacearum based on its 16S rRNA gene sequence. J12 could produce 2,4-diacetylphloroglucinol (2,4-DAPG), hydrogen cyanide (HCN), siderophore(s) and protease. The maximum growth and antagonistic activity were recorded at 30 degrees C and pH 8. Glucose and tryptone were used as the most suitable carbon and nitrogen sources, respectively. Strain J12 significantly suppressed tomato bacteria wilt by 45.5% in the greenhouse experiment. The main antimicrobial compound 0012 was identified as 2,4-diacetylphloroglucinol (2,4-DAPG) by HPLC-ESI-MS analysis. The gene cluster phlACBD, which is responsible for 2,4-DAPG production, was identified and expressed in the bacterial strain Escherichia coli DH5 alpha. (C) 2012 Elsevier GmbH. All rights reserved.
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