Article
Chemistry, Multidisciplinary
Byoung-Hoon Kang, Kyung-Won Jang, Eun-Sil Yu, Hamin Na, Yun-Jae Lee, Woong-Yeol Ko, NamHo Bae, Donggee Rho, Ki -Hun Jeong
Summary: This study presents a rapid and compact real-time RT-PCR system for decentralized molecular diagnostics. It utilizes plasmonic nucleic acid amplification and quantification, and features an ultrafast plasmonic thermocycler, a disposable plastic-on-metal cartridge, and a compact fluorescence microscope. The system successfully enables 10-minute diagnosis of COVID-19 with high amplification efficiency, classification accuracy, and total percent agreement for clinical diagnostic tests.
Article
Chemistry, Multidisciplinary
Byoung-Hoon Kang, Kyung-Won Jang, Eun-Sil Yu, Hamin Na, Yun-Jae Lee, Woong-Yeol Ko, NamHo Bae, Donggee Rho, Ki-Hun Jeong
Summary: This study presents an ultrafast plasmonic nucleic acid amplification and real-time quantification system for decentralized molecular diagnostics. The system features ultrafast photothermal cycling, rapid heat transfer, and efficient PCR quantification, along with high-contrast fluorescence microscopic imaging. It enables decentralized point-of-care molecular diagnostic testing in primary care and developing countries.
Article
Immunology
Di Wang, Xinya Jiao, Haijiang Jia, Shumei Cheng, Xi Jin, Youhua Wang, Yunhua Gao, Xiaofeng Su
Summary: This study developed a droplet digital PCR (ddPCR) assay for accurate and sensitive detection and quantification of Verticillium dahliae and V. longisporum, showing that ddPCR is more sensitive and accurate than qPCR for quantifying trace amounts of these pathogens in field samples.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Genetics & Heredity
Sabina Maria Bruno, Giovanna Blacona, Stefania Lo Cicero, Germana Castelli, Mariarita Virgulti, Giancarlo Testino, Silvia Pierandrei, Andrea Fuso, Giuseppe Cimino, Giampiero Ferraguti, Adriana Eramo, Marco Lucarelli
Summary: In the precision medicine era of cystic fibrosis (CF), accurate quantification of CFTR gene expression is crucial for personalized therapeutic approaches. This paper compares two methods for quantifying CFTR gene expression and discusses their advantages and limitations.
Article
Microbiology
Junxia Feng, Xiaohu Cui, Bing Du, Hanqing Zhao, Yanling Feng, Jinghua Cui, Chao Yan, Lin Gan, Zheng Fan, Tongtong Fu, Ziying Xu, Zihui Yu, Rui Zhang, Shuheng Du, Ziyan Tian, Qun Zhang, Guanhua Xue, Jing Yuan
Summary: This study developed a rapid and sensitive ddPCR assay for detecting Klebsiella pneumoniae in fecal samples. The ddPCR assay showed higher sensitivity, specificity, and lower inhibition compared to real-time PCR and microbial culture. The findings suggest that ddPCR could be an effective tool for detecting K. pneumoniae in fecal samples and guiding treatment decisions.
MICROBIOLOGY SPECTRUM
(2023)
Article
Food Science & Technology
Chenxi Huang, Binti Youssouf Mahboubat, Yifeng Ding, Qile Yang, Jia Wang, Min Zhou, Xiaohong Wang
Summary: The study developed a rapid and accurate detection method for Salmonella using the lytic Siphovirus phage LPST10, which showed a detection limit of <30 CFU/mL in different food matrices. The method involves magnetic separation and real-time PCR detection, allowing for quick and efficient detection of Salmonella in complex food samples.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2021)
Article
Agronomy
Stefania Fontanazza, Alessia Restuccia, Giovanni Mauromicale, Aurelio Scavo, Cristina Abbate
Summary: The study aimed to identify microorganisms responsible for degrading BP mulch films through a combination of biodegradation and molecular tests. A field trial revealed a higher abundance of Pseudomonas putida in BP plots, showing potential for accelerating the degradation of BP mulch films and reducing plastic pollution in agriculture.
Article
Hematology
Sanyun Wu, Yixuan Li, Ruiying Huang, Ting Li, Yalan Yu, Ping Luo, Xuelan Zuo, Li He
Summary: The early diagnosis and intervention of myeloproliferative neoplasm (MPN) are crucial for prognosis. This study compared the performance of real-time quantitative PCR (qPCR) and droplet digital PCR (ddPCR) in detecting the burden of JAK2V617F mutation. The results showed that ddPCR outperformed qPCR in terms of accuracy, sensitivity, and stability, particularly in low burden cases.
ANNALS OF HEMATOLOGY
(2023)
Article
Virology
Yi Huang, Shuqi Xiao, Zhiming Yuan
Summary: The study compared and evaluated the sensitivity, reproducibility, and specificity of 23 in-house assays for ebolavirus detection, finding that targeting the more conservative NP gene may offer better sensitivity for EVD case definition and quantitative measurement. This analysis could contribute to the standardization of ebolavirus detection and quantification assays, providing a better understanding of results and making implementation easier, particularly during outbreaks.
Article
Food Science & Technology
Sara Arnaboldi, Roberto Benevenia, Barbara Bertasi, Elisa Galuppini, Lucia Mangeri, Michela Tilola, Daniela Bassi, Pier Sandro Cocconcelli, Angelo Stroppa, Giorgio Varisco
Summary: The validation of a real-time PCR method targeting C. tyrobutyricum for the detection of contaminants in milk has been conducted, confirming its detection rate and providing strong support for quality control in the dairy industry.
Article
Microbiology
Agata Anna Cisek, Iwona Bak, Bozena Cukrowska
Summary: Methanogenic archaea are important but often ignored in studies of human and animal intestinal microbiota. This research focused on improving the quantification of methanogens using qPCR, by refining the protocol and increasing the specificity and sensitivity of the assay. The results showed increased detection range and successful validation parameters, allowing for improved detection and quantification of methanogens in stool samples, such as chicken droppings in this study.
Article
Chemistry, Applied
Joana Costa, Caterina Villa, Liliana Grazina, Isabel Mafra
Summary: This study proposes two highly sensitive and specific single-tube nested real-time PCR methods for the analysis of cashew nut as an allergenic food. The method targeting the Ana o 3 gene showed the highest relative sensitivity in dough/biscuit and was successfully validated for precision and accuracy.
Article
Chemistry, Multidisciplinary
Bong Kyun Kim, Sang-A Lee, Minju Park, Eui Ju Jeon, Mi Jung Kim, Jung Min Kim, Heesuk Kim, Seungwon Jung, Sang Kyung Kim
Summary: This article introduces a photonic PCR platform using hydrogel microparticles, which utilizes photothermal nanomaterials as heating elements for rapid and portable POCT. The researchers successfully demonstrate the ability to perform multiplex assays by loading multiple encoded pPIN microparticles in a single reaction.
Article
Microbiology
Alexandra Puertolas, Peter J. M. Bonants, Eric Boa, Steve Woodward
Summary: Numerous outbreaks of Phytophthora and Pythium diseases have occurred in Europe due to introduction of contaminated ornamental plants. Detection and identification of pathogens are crucial. Results showed that oomycetes were isolated from 89.9% of plants using classical techniques, and real-time PCR demonstrated that oomycetes were associated with all plants tested.
Article
Food Science & Technology
Mirian Labrador, Carlota Gimenez-Rota, Carmen Rota
Summary: A real-time PCR method has been developed for quantifying Listeria monocytogenes on meat products through the gene hlyA, achieving high efficiency and specificity. The method, combined with matrix lysis, showed excellent correspondence with the reference method and higher precision and sensitivity at low concentrations of the pathogen, demonstrating potential for routine control in the meat industry.
Article
Microbiology
Vera Analise Schommer, Mateus Torres Nazari, Flavia Melara, Julia Catiane Arenhart Braun, Alan Rempel, Lara Franco dos Santos, Valdecir Ferrari, Luciane Maria Colla, Aline Dettmer, Jeferson Steffanello Piccin
Summary: This review introduces the techniques and mechanisms of bacteria immobilization on biochar, as well as its applications in bioremediation and agriculture. The immobilization characteristics of biochar depend on pyrolysis methods, raw materials, and properties of biochar. Scanning electron microscope and colony forming unit analysis are commonly used to verify the immobilization efficiency. Applying biochar-immobilized bacteria can improve soil quality, plant growth, and crop yield.
MICROBIOLOGICAL RESEARCH
(2024)
Article
Microbiology
Manoj Kumar Solanki, Naveen Chandra Joshi, Prashant Kumar Singh, Sandeep Kumar Singh, Gustavo Santoyo, Lucas Carvalho Basilio de Azevedo, Ajay Kumar
Summary: The rhizosphere microbiome plays a crucial role in plant growth and health. Rhizosphere engineering can be used to modify the microbiome and improve plant productivity and resilience.
MICROBIOLOGICAL RESEARCH
(2024)
Article
Microbiology
Xin Cao, Xing-Wang Cheng, Yin-Ying Liu, Hong-Wei Dai, Ren-You Gan
Summary: Maintaining oral health is crucial for overall well-being. Imbalances in oral microorganisms can lead to various infectious diseases. Exploring alternatives for prevention and management of oral infections is important due to limitations of current treatments. Natural products offer potential as reliable, effective, and affordable therapeutic options for oral infectious diseases.
MICROBIOLOGICAL RESEARCH
(2024)
Article
Microbiology
Jose David Flores-Felix, Ana Carolina Goncalves, Sara Meirinho, Ana Raquel Nunes, Gilberto Alves, Cristina Garcia-Viguera, Diego A. Moreno, Luis R. Silva
Summary: The application of bacterial biofortifiers is increasing and has been shown to improve the nutritional characteristics of blueberries in Portugal.
MICROBIOLOGICAL RESEARCH
(2024)
Review
Microbiology
Asma Sani, Wan-Qi Qin, Jia-Yi Li, Yi-Fan Liu, Lei Zhou, Shi-Zhong Yang, Bo-Zhong Mu
Summary: This article summarizes the structural diversity of microbial lipopeptide biosurfactants and their applications as biocontrol agents in plants. Lipopeptides have low toxicity and wide range of applications, making them important in the pesticide industry.
MICROBIOLOGICAL RESEARCH
(2024)
Article
Microbiology
Xiao-Yu Sun, Jie Deng, Chenhui Zhang, Sin-Yee Fung, Kam-Leung Siu, Ying-Ying Cheng, Liumei Ye, Jiaoxia Qin, Ke Wang, Jiu-Xin Qu, Wenying Gao, Fuxiang Wang, Dong-Yan Jin, Liang Yang
Summary: In this study, a novel c-di-GMP binding protein SodA that regulates oxidative stress tolerance in S. maltophilia was identified. The enzyme activity of SodA was shown to be directly modulated by c-di-GMP through various in vivo and in vitro experiments.
MICROBIOLOGICAL RESEARCH
(2024)
