期刊
MICROBIAL PATHOGENESIS
卷 46, 期 5, 页码 235-242出版社
ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
DOI: 10.1016/j.micpath.2009.01.006
关键词
Chlamydia pneumoniae; Microarray; Infection model; Lymphocyte
资金
- The Academy of Finland [202491, 202492, 110340, 119004]
- Academy of Finland (AKA) [202492, 202491, 119004, 202491, 202492, 119004] Funding Source: Academy of Finland (AKA)
in this study experimental mouse model for Chlamydia pneumoniae infection was used to elucidate the nature of immune response developing during primary and secondary infection. First we examined the mononuclear cells from different lymphoid organs in BALB/c mice during C. pneumoniae infection and detected a strong lymphocyte influx into mediastinal lymph nodes (MLN). To further characterize the C pneumoniae induced immune response the gene expression profiles of MLN derived lymphocytes was studied. To identify genes characteristic for reinfection we compared gene expression profiles during reinfection and primary infection and found 148 genes to be differentially regulated in CD19+ cells, 7 in CD4+ cells and 12 in CD8+ cells. A panel of these genes was selected to be confirmed by real-time RTPCR. Genes related to interferon signaling like Ifit1, Ifit3, Gbp2, Irf7 and Usp18 were found to be upregulated when reinfection was compared to primary infection. In our study we were able to identify 8 genes that were differentially expressed between reinfection and primary infection in lymphocytes. These novel gene expression signatures provide new insights and clues to the nature of protective immunity established during experimental C pneumoniae immunity. (C) 2009 Elsevier Ltd. All rights reserved.
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