Article
Veterinary Sciences
Ahmed Elfadadny, Jumpei Uchiyama, Kazuyoshi Goto, Ichiro Imanishi, Rokaia F. Ragab, Wedad M. Nageeb, Keita Iyori, Yoichi Toyoda, Toshihiro Tsukui, Kaori Ide, Keiko Kawamoto, Koji Nishifuji
Summary: The strong bond between dogs and their owners could lead to the transfer of antibiotic-resistant bacteria from dogs to humans. In Japan, there have been limited studies on the bacterium Pseudomonas aeruginosa in dogs. We assessed the antimicrobial resistance profile and genotype of P. aeruginosa isolated from the ear canal of dogs in Japan in 2020.
FRONTIERS IN VETERINARY SCIENCE
(2023)
Article
Microbiology
Pabllo Antonny Silva Dos Santos, Yan Correa Rodrigues, Davi Josue Marcon, Amalia Raiana Fonseca Lobato, Thalyta Braga Cazuza, Maria Isabel Montoril Gouveia, Marcos Jesse Abrahao Silva, Alex Brito Souza, Luana Nepomuceno Gondim Costa Lima, Ana Judith Pires Garcia Quaresma, Danielle Murici Brasiliense, Karla Valeria Batista Lima
Summary: This study aims to determine the antimicrobial resistance, virulence, and genotyping features of P. aeruginosa strains producing SPM-1 in the Northern region of Brazil. The PCR technique was used to determine the presence of virulence and resistance genes. The Kirby-Bauer disk diffusion method was performed to assess the susceptibility profile of antimicrobials, and the MLST technique was used for genotyping.
Review
Microbiology
Pabllo Antonny Silva Dos Santos, Marcos Jesse Abrahao Silva, Maria Isabel Montoril Gouveia, Luana Nepomuceno Gondim Costa Lima, Ana Judith Pires Garcia Quaresma, Patricia Danielle Lima De Lima, Danielle Murici Brasiliense, Karla Valeria Batista Lima, Yan Correa Rodrigues
Summary: The purpose of this study was to describe the prevalence of M beta L-producing Pseudomonas aeruginosa (PA) isolates and the frequency of bla(SPM-1) in M beta L-PA-producing isolates in Brazil. The findings showed that M beta L-PA and SPM-1 isolates were relatively common among individuals in Brazil. Further research is needed to identify risk factors, reservoirs, transmission pathways, and the origin of infection.
Article
Microbiology
Yiwei Zhu, Jie Chen, Han Shen, Zhongju Chen, Qi-Wen Yang, Jin Zhu, Xi Li, Qing Yang, Feng Zhao, Jingshu Ji, Heng Cai, Yue Li, Linghong Zhang, Sebastian Leptihn, Xiaoting Hua, Yunsong Yu
Summary: Klebsiella pneumoniae carbapenemase (KPC)-producing Pseudomonas aeruginosa (KPC-PA) strains were found in 40.4% of carbapenem-resistant P. aeruginosa (CRPA) isolates, with half of them resistant to ceftazidime-avibactam (CAZ-AVI). Eight plasmid types and two mobile genetic elements mediating bla(KPC-2) transmission were identified through sequencing. Curing bla(KPC-2) plasmids in 28 strains restored CAZ-AVI susceptibility, indicating its role in resistance.
Article
Infectious Diseases
Maria Giufre, Elena Mazzolini, Marina Cerquetti, Silvio Brusaferro
Summary: This study compared ESBL-producing Escherichia coli isolates from humans and food-producing animals, finding different types of ESBLs in different sources. The results suggest the potential exchange of ESBL genes from animals to humans, emphasizing the need for strict monitoring based on a 'One Health' approach.
INTERNATIONAL JOURNAL OF ANTIMICROBIAL AGENTS
(2021)
Article
Microbiology
Scott A. Cunningham, Allison R. Eberly, Stephan Beisken, Andreas E. Posch, Audrey N. Schuetz, Robin Patel
Summary: Over the past decade, whole-genome sequencing has become the dominant method for studying genetic relatedness, surpassing traditional bacterial typing methods. These sequencing data can also be utilized in predicting antibiotic resistance, with high accuracy. These methods have the potential to be used in clinical laboratories to overcome the challenges of phenotypic susceptibility testing.
MICROBIOLOGY SPECTRUM
(2022)
Article
Infectious Diseases
Melise Chaves Silveira, Rodolpho Mattos Albano, Claudio Marcos Rocha-de-Souza, Robson Souza Leao, Elizabeth Andrade Marques, Renata Cristina Picao, Gabriela Bergiante Kraychete, Ivson Cassiano de Oliveira Santos, Thamirys Rachel Tavares e Oliveira, Camila Bastos Tavares-Teixeira, Ana Paula D' Alincourt Carvalho-Assef
Summary: The high rates of carbapenem resistance among Brazilian Pseudomonas aeruginosa isolates are mainly associated with the clone ST277 producing the carbapenemase SPM-1. The complete genetic composition of a IncP plasmid harboring blaKPC-2 in isolates of this endemic clone carrying chromosomal blaSPM-1 was described using whole genome sequencing. These results confirm the association of these two carbapenemases in ST277 and also describe the genetic composition of a novel blaKPC-2-plasmid. Considering the fact that this association occurs in a high-risk clone, monitoring the dissemination of this plasmid should be a public health concern.
INFECTION GENETICS AND EVOLUTION
(2022)
Article
Agronomy
Gaber Attia Abo-Zaid, Ahmed Salah Abdullah, Nadia Abdel-Mohsen Soliman, Ebaa Ebrahim El-Sharouny, Abdulaziz A. Al-Askar, Yiming Su, Ahmed Abdelkhalek, Soraya Abdel-Fattah Sabry
Summary: The secretion of siderophores by Pseudomonas aeruginosa F2 and P. fluorescens JY3 was evaluated and found to have an inhibitory effect on Fusarium oxysporum and Rhizoctonia solani. Two optimized media were used to produce siderophores as biocontrol agents, and the cell-free supernatants containing siderophores were used to prepare bio-friendly formulations. These formulations showed significant reduction in damping-off caused by F. oxysporum and R. solani, as well as promoting the growth of wheat plants.
Article
Immunology
Biying Zhang, Xun Xu, Xiaomei Song, Yicheng Wen, Zhichen Zhu, Jingnan Lv, Xiaofang Xie, Liang Chen, Yi-Wei Tang, Hong Du
Summary: The study revealed the shift of CRPA from ST697 to ST463 in East China, with ST463 showing higher drug resistance, posing greater challenges for clinical management.
EMERGING MICROBES & INFECTIONS
(2022)
Article
Infectious Diseases
Kamrul Islam, Fekade B. Sime, Steven C. Wallis, Michelle J. Bauer, Brian M. Forde, Patrick Harris, Tahmina Shirin, Zakir H. Habib, Meerjady S. Flora, Jason A. Roberts
Summary: This study compared the pharmacodynamics of piperacillin/tazobactam and meropenem against ESBL-producing and non-producing E. coli. The results suggest that piperacillin/tazobactam may be a potential alternative to carbapenems in treating urosepsis caused by ESBL-producing E. coli.
JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY
(2022)
Article
Infectious Diseases
Miki Takemura, Mark G. Wise, Meredith A. Hackel, Daniel F. Sahm, Yoshinori Yamano
Summary: This study evaluated the in vitro antibacterial activity of siderophore cephalosporin cefiderocol against MBL-producing clinical isolates. The results showed that cefiderocol exhibited potent in vitro activity against MBL-producing strains of different bacterial species.
JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY
(2023)
Article
Immunology
Sandip Patil, Xiaowen Chen, Shaowei Dong, Huirong Mai, Bruno Silvester Lopes, Sixi Liu, Feiqiu Wen
Summary: This study investigated the distribution and antibiotic resistance mechanisms of 294 clinical isolates of Pseudomonas aeruginosa in a pediatric hospital in China. The results showed that most isolates were resistant to multiple antibiotics, including beta-lactam drugs. Multiple beta-lactamases and plasmids were detected, suggesting that the resistance of the isolates may be due to clonal spread and dissemination.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2023)
Article
Public, Environmental & Occupational Health
Ori Berger, Mor N. Lurie-Weinberger, Evgenia Tsyba, Ran Talisman
Summary: Medical tourism for affordable aesthetic surgeries in countries like Turkey can lead to infections such as carbapenem-resistant Pseudomonas aeruginosa. Bioinformatics analysis of a reported case in Israel showed a highly virulent and antibiotic-resistant strain, highlighting the importance of infection control and appropriate antibiotic use in medical tourism.
JOURNAL OF TRAVEL MEDICINE
(2023)
Article
Microbiology
Muhammad Ali Syed, Bushra Jamil, Hazem Ramadan, Maria Rukan, Shahzad Ali, Shahid Ahmad Abbasi, Tiffanie A. Woodley, Charlene R. Jackson
Summary: The study characterized Staphylococcus aureus isolates from a tertiary care hospital in Rawalpindi, Pakistan, and found high levels of resistance, particularly for MRSA strains. The majority of S. aureus isolates belonged to specific spa types, with the most common clone identified as ST772-t657 (Bengal Bay clone).
Article
Immunology
Fred C. Tenover, David P. Nicolau, Christian M. Gill
Summary: The article discusses the clinical importance of carbapenem-resistant Pseudomonas aeruginosa (CR-PA) worldwide, including the resistance situation of the pathogen, transmission routes, different types of carbapenemases in various regions, and strategies for optimizing treatment.
EMERGING MICROBES & INFECTIONS
(2022)
Article
Infectious Diseases
Jhonatha Rodrigo Cordeiro-Moura, Lorena Cristina Correa Fehlberg, Carolina Silva Nodari, Adriana Pereira de Matos, Vinicius de Oliveira Alves, Rodrigo Cayo, Ana Cristina Gales
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2020)
Review
Infectious Diseases
Willames M. B. S. Martins, Mark A. Toleman, Ana C. Gales
BRAZILIAN JOURNAL OF INFECTIOUS DISEASES
(2020)
Article
Infectious Diseases
Patricia de Rossi, Sergio Cimerman, Jose Carlos Truzzi, Clovis Arns da Cunha, Rosiane Mattar, Marines Dalla Valle Martino, Mauricio Hachul, Adagmar Andriolo, Jose Ananias Vasconcelos Neto, Joao Antoio Pereira-Correia, Antonia M. O. Machado, Ana Cristina Gales
BRAZILIAN JOURNAL OF INFECTIOUS DISEASES
(2020)
Article
Pharmacology & Pharmacy
Aghata Cardoso da Silva Ribeiro, Willames Marcos Brasileiro da Silva Martins, Adilson Aderito da Silva, Ana Cristina Gales, Daniela Goncales Galasse Rando, Luciene Andrade da Rocha Minarini
EUROPEAN JOURNAL OF PHARMACEUTICAL SCIENCES
(2020)
Letter
Infectious Diseases
Willames M. B. S. Martins, Bruna M. S. Seco, Jorge L. M. Sampaio, Kirsty Sands, Mark A. Toleman, Ana C. Gales
INTERNATIONAL JOURNAL OF ANTIMICROBIAL AGENTS
(2020)
Article
Infectious Diseases
Gabriel T. Cuba, Gerlan Rocha-Santos, Rodrigo Cayo, Ana Paula Streling, Carolina S. Nodari, Ana C. Gales, Antonio C. C. Pignatari, David P. Nicolau, Carlos R. V. Kiffer
JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY
(2020)
Article
Infectious Diseases
Bruno C. Boettger, Rodrigo Cayo, Ana Paula Streling, Carolina S. Nodari, Luiz G. P. Almeida, Willames M. B. S. Martins, Raquel Girardello, Ana Tereza R. Vasconcelos, Ana C. Gales, Antonio C. C. Pignatari
Summary: Distinct extensively drug-resistant A. baumannii clones can circulate in the same hospital setting during a short time period, illustrating a very complex epidemiological scenario for this priority pathogen.
MICROBIAL DRUG RESISTANCE
(2021)
Article
Environmental Sciences
Ana Clara Narciso, Willames M. B. S. Martins, Luiz G. P. Almeida, Rodrigo Cayo, Stefanie Vanessa Santos, Patricia Locosque Ramos, Nilton Lincopan, Ana Tereza R. Vasconcelos, Ana Cristina Gales
SCIENCE OF THE TOTAL ENVIRONMENT
(2020)
Article
Microbiology
Jullia A. S. Nascimento, Fernanda F. Santos, Tiago B. Valiatti, Jose F. Santos-Neto, Ana Carolina M. Santos, Rodrigo Cayo, Ana C. Gales, Tania A. T. Gomes
Summary: This study identified 9 out of 452 UPEC strains (2%) as hybrid strains, with 7 classified as UPEC/EAEC and 2 as UPEC/aEPEC. These hybrid strains displayed specific characteristics in terms of cell adhesion ability and virulence potential.
Article
Microbiology
Leticia B. Migliorini, Romario O. de Sales, Paula C. M. Koga, Andre M. Doi, Anja Poehlein, Alexandra R. Toniolo, Fernando G. Menezes, Marines D. Martino, Ana C. Gales, Holger Brueggemann, Patricia Severino
Summary: This study reports the genomic context of KPC coding genes bla(KPC-2), bla(KPC-3) and bla(KPC-30) in multidrug-resistant Klebsiella pneumoniae isolates from Brazil, with a focus on the prevalence and dissemination of bla(KPC)-harboring plasmids. It demonstrates the risk of horizontal spread of KPC coding genes and the high synteny of plasmid backbones among isolates, including the recent spread of bla(KPC-3) and bla(KPC-30).
Article
Infectious Diseases
Willames M. B. S. Martins, Michael H. Lenzi, Ana C. Narciso, Priscila P. Dantas, Diego O. Andrey, Qiu E. Yang, Kirsty Sands, Eduardo A. Medeiros, Timothy R. Walsh, Ana C. Gales
Summary: This study describes the undetected outbreak of a rare carbapenemase BKC-1-producing Klebsiella pneumoniae ST442 clone, occurring in a Brazilian hospital. The misidentification of BKC-1 may have contributed to the spread of resistant clones, emphasizing the importance of correct and rapid identification of antimicrobial resistance mechanisms in hospitals.
INTERNATIONAL JOURNAL OF ANTIMICROBIAL AGENTS
(2022)
Article
Infectious Diseases
Aghata Cardoso da Silva Ribeiro, Fernanda Fernandes Santos, Ikechukwu Benjamin Moses, Luciene Andrade da Rocha Minarini, Ana Cristina Gales
Summary: In this study, a new fast, economic, and effective typing technique for carbapenem-resistant Klebsiella pneumoniae (CR-KPN) was explored by analyzing the fosA(KP) sequence. The results showed that chromosomal fosA(KP) sequences are highly conserved in different STs and CCs, providing a useful tool to identify the epidemiological STs for KPN and discriminate them from CC258/11.
MICROBIAL DRUG RESISTANCE
(2022)
Letter
Infectious Diseases
Tiago Barcelos Valiatti, Fernanda Fernandes Santos, Andre Valencio, Rodrigo Cayo, Tania Aparecida Tardelli Gomes, Ana Cristina Gales
JOURNAL OF GLOBAL ANTIMICROBIAL RESISTANCE
(2022)
Article
Infectious Diseases
Aghata Cardoso da Silva Ribeiro, Yohanna Carvalho dos Santos Aoun Chikhani, Tiago Barcelos Valiatti, Andre Valencio, Mariana Neri Lucas Kurihara, Fernanda Fernandes Santos, Luciene Andrade da Rocha Minarini, Ana Cristina Gales
Summary: In this study, the activity of fosfomycin in combination with meropenem or polymyxin B against KPC-KPN was evaluated. The results showed that the combination of fosfomycin and meropenem restored meropenem activity and exhibited a synergistic effect with fosfomycin and polymyxin B. In the Galleria mellonella model, the combination of fosfomycin and meropenem had a higher survival rate for larvae, while fosfomycin and polymyxin B combination had a more significant biofilm disruption effect.
Article
Infectious Diseases
Tobias Manuel Appel, Maria Fernanda Mojica, Elsa De La Cadena, Christian Jose Pallares, Marcela A. Radice, Paulo Castaneda-Mendez, Diego A. Jaime-Villalon, Ana C. Gales, Jose M. Munita, Maria Virginia Villegas