Article
Infectious Diseases
Toyotaka Sato, Shin-ichi Yokota, Tooru Tachibana, Satoshi Tamai, Shigeki Maetani, Yutaka Tamura, Motohiro Horiuchi
Summary: The study found that companion animals can carry fluoroquinolone-resistant Escherichia coli, which may be associated with human clinical infections and can potentially be transmitted to their owners.
Article
Immunology
David Henry Greentree, Louis B. Rice, Curtis J. Donskey
Summary: There is an urgent need for surveillance to determine if C. difficile strains with reduced vancomycin susceptibility are circulating in other areas, as high-level phenotypic resistance to vancomycin has recently been reported in multiple geographic regions.
CLINICAL INFECTIOUS DISEASES
(2022)
Article
Microbiology
Mayo Yasugi, Shingo Hatoya, Daisuke Motooka, Daisuke Kondo, Hideo Akiyoshi, Masayuki Horie, Shota Nakamura, Terumasa Shimada
Summary: The presence of mcr plasmid-mediated colistin-resistant ESBL-producing Enterobacterales in companion dogs and cats in Japan was identified. The isolates from a dog and a cat carried different mcr genes and bla genes on plasmids. This suggests that companion dogs and cats may serve as important reservoirs for cross-species transmission of the mcr gene.
VETERINARY MICROBIOLOGY
(2023)
Article
Multidisciplinary Sciences
Ryan T. T. Searcy, Jacob R. R. Phaneuf, Alexandria B. B. Boehm
Summary: Fecal indicator bacteria (FIB) are monitored to assess water quality and health risk at beaches, but infrequent monitoring can lead to inaccurate assessments. This study conducted high-frequency FIB sampling at an enclosed harbor in California, showing more variability in FIB concentrations compared to open beach sites. Correlation and regression analyses revealed strong associations between FIB concentrations and chlorophyll a, turbidity, wind speed, and tide level. The study emphasizes the importance of sampling FIB concentrations and environmental parameters at appropriate temporal resolutions for water quality monitoring.
Article
Infectious Diseases
Jiayu Shao, Hangdong Dai, Liwei Xu, Shuilong Zhu, Jufang Zhu, Hangyu Fu, Minxia Ge, Xianhong He
Summary: The purpose of this study was to investigate the genomic characteristics and antimicrobial resistance mechanisms of ESBL-producing E. coli isolates recovered from a district hospital in China. The results showed that these isolates exhibited resistance to multiple antibiotics, with the majority belonging to the ST131 serotype and having different virulence genes and sequence types. Therefore, continuous surveillance and control of the transmission of these multi-drug resistant bacteria in clinical and community settings are crucial.
INFECTION AND DRUG RESISTANCE
(2023)
Article
Agriculture, Dairy & Animal Science
Zui Wang, Qin Lu, Xiaohui Mao, Li Li, Junfeng Dou, Qigai He, Huabin Shao, Qingping Luo
Summary: ESBL-producing E.coli strains isolated from chickens in central China showed high resistance to cephalosporins and harbored diverse ESBL-encoding genes. These strains pose a significant public health risk.
Article
Multidisciplinary Sciences
Maria Muddassir, Almas Raza, Sadaf Munir, Ahmad Basirat, Muddassir Ahmed, Mazia Shahid Butt, Omair Arshad Dar, Syed Shoaib Ahmed, Saba Shamim, Syed Zeeshan Haider Naqvi
Summary: This study investigated the antibiogram and drug resistance genes of carbapenem-resistant Pseudomonas aeruginosa, as well as the potency of Silver Nanoparticles against multidrug-resistant strains. The results showed that the conjugated effect of silver nanoparticles with antibiotics effectively inhibited multidrug-resistant Pseudomonas aeruginosa.
SCIENTIFIC REPORTS
(2022)
Article
Infectious Diseases
Chien Chuang, Kuei-Chuan Lee, Yen-Po Wang, Pei-Chang Lee, Tien-En Chang, Yi-Hsiang Huang, Yi-Tsung Lin, Ming-Chih Hou
Summary: A stringent donor screening program is essential for reducing the risk of pathogen transmission in fecal microbiota transplantation (FMT). High carriage rates of third-generation cephalosporin-resistant (3GCR) Enterobacterales and diarrheagenic E. coli were observed in donors, and the effectiveness of multiplex PCR screening panels in FMT donor screening programs was confirmed.
EUROPEAN JOURNAL OF CLINICAL MICROBIOLOGY & INFECTIOUS DISEASES
(2023)
Article
Multidisciplinary Sciences
Hasan Ejaz, Sonia Younas, Khalid O. A. Abosalif, Kashaf Junaid, Badr Alzahrani, Abdullah Alsrhani, Abualgasim Elgaili Abdalla, Muhammad Ikram Ullah, Muhammad Usman Qamar, Sanaa S. M. Hamam
Summary: The colonization of ESBL-producing Enterobacteriaceae in animal gut microbiota poses a significant global threat, with a high prevalence of bla(CTX-M), bla(TEM), and bla(SHV) variants among enterobacteria. These genes, along with integrons, contribute to extensive drug resistance in human strains, highlighting the limited therapeutic options available.
Article
Immunology
Esther Rios, Maria Del Carmen Lopez Diaz, Esther Culebras, Iciar Rodriguez-Avial, Carmen Rodriguez-Avial
Summary: In this study, the in vitro activity of fosfomycin against Escherichia coli isolated from urine samples in 2013, 2018, and 2021 was evaluated. The results showed an increasing trend of fosfomycin resistance in E. coli urinary isolates, which was associated with extended-spectrum beta-lactamases (ESBL) production.
MEDICAL MICROBIOLOGY AND IMMUNOLOGY
(2022)
Article
Immunology
Achiraya Siriphap, Thawatchai Kitti, Akachai Khuekankaew, Chalermchai Boonlao, Chonthida Thephinlap, Chutamas Thepmalee, Nittiya Suwannasom, Krissana Khoothiam
Summary: This study investigated the prevalence of ESBL-positive E. coli and K. pneumoniae isolates from hospitalized patients in Chiangrai Prachanukroh hospital, Thailand. The study found a high prevalence and emerging antibiotic resistance of ESBL-positive strains, indicating the need for carbapenems and aminoglycosides as the first-line drugs.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Microbiology
Mireia Lopez-Siles, Zaira Moure, Aly Salimo Muadica, Sergio Sanchez, Raquel Cruces, Alicia avila, Noelia Lara, Pamela Carolina Koster, Alejandro Dashti, Jesus Oteo-Iglesias, David Carmena, Michael J. McConnell
Summary: This study aimed to identify external factors associated with ESBL-E carriage and characterize clonal relationships and mechanisms of resistance in ESBL-E isolates. Among the 887 healthy children surveyed, 24 ESBL-E strains were detected, mainly Escherichia coli. The prevalence of ESBL-E in children was found to be lower than the average colonization rate previously observed in Europe for both children and adults, with CTX-M being the most frequently identified ESBL type.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Fisheries
Soon Bum Shin, Sung Rae Cho, Jong Soo Mok
Summary: The safety of seafood is a critical public health concern in Korea due to the high rate of raw seafood consumption. A study was conducted in 2018 to investigate the prevalence and antimicrobial resistance of fecal-associated bacteria in seawater samples and aquatic animals collected from aquaculture farms along the Korean coast. The results showed that Escherichia coli was the most prevalent and exhibited resistance to multiple antimicrobials.
AQUACULTURE RESEARCH
(2023)
Article
Microbiology
Angel Rodriguez-Villodres, Alicia Gutierrez Linares, Lydia Galvez-Benitez, Jeronimo Pachon, Jose Antonio Lepe, Younes Smani
Summary: A method for detecting TZP resistance and ESRI in E. coli was designed and validated, showing great efficacy in detecting TZP-intermediate or -resistant isolates and TZP-susceptible isolates with the capacity for ESRI development. The method could potentially reduce health costs, ineffective treatments, and inappropriate antibiotic use.
MICROBIOLOGY SPECTRUM
(2021)
Article
Food Science & Technology
Seyda Sahin, Mahmut Niyazi Mogulkoc, Cemil Kurekci
Summary: This study found that extended spectrum beta-lactamase producing Escherichia coli isolates from chicken meat showed resistance to disinfectants and heavy metals, and carried resistance genes. This poses challenges for disinfection in the poultry industry in Turkey.
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY
(2022)
Review
Environmental Sciences
B. Guimaraes, J. Roembke, M. J. B. Amorim
Summary: Standard laboratory tests, although feasible and providing robust results, may underestimate the potential effects of prolonged exposures to stressors, especially persistent contaminants. Recent studies have focused on prolonged exposure, but with varying test designs. It is recommended to include long-term tests in existing regulatory requirements and adapt the designs for representative soil invertebrate species.
SCIENCE OF THE TOTAL ENVIRONMENT
(2023)
Article
Environmental Sciences
Angela Barreto, Joana Santos, Lara Almeida, Vitor Tavares, Edgar Pinto, Maria Celeiro, Carmen Garcia-Jares, Vera L. Maria
Summary: The main aim of this study is to understand the effects of nanoplastics on the terrestrial invertebrate Folsomia candida. The research shows that the presence of nanoplastics can alter the toxicity of industrial chemicals and pharmaceuticals to the organism. However, the effects vary depending on the concentration and conditions.
Article
Ecology
Idris Nasir Abdullahi, Guillermo Juarez-Fernandez, Ursula Hofle, Javier Latorre-Fernandez, Teresa Cardona-Cabrera, David Minguez-Romero, Myriam Zarazaga, Carmen Lozano, Carmen Torres
Summary: This study investigated the nasotracheal carriage rates of Staphylococcus aureus in white storks in Southern Spain and characterized the genetic diversity of the isolates. It found that approximately 31% of white storks carried S. aureus, with nasal samples having a higher carriage rate (36.5%) than tracheal samples (11.9%). All isolates were susceptible to methicillin, and a small percentage (8.8%) were susceptible to all tested antibiotics. Various spa types and clonal complexes were detected, including the emergence of MSSA-CC398 and toxigenic MSSA strains in stork nestlings.
Article
Microbiology
Rosa Fernandez-Fernandez, Idris Nasir Abdullahi, Carmen Gonzalez-Azcona, Adriana Ulloa, Agusti Martinez, Sara Garcia-Vela, Ursula Hofle, Myriam Zarazaga, Carmen Lozano, Carmen Torres
Summary: A total of 259 staphylococci strains were isolated from nasotracheal samples of 87 healthy nestling white storks, and their antimicrobial activity was tested using the spot-on-lawn method. Extracts from these strains were also tested against indicator bacteria. Results showed that 9 strains exhibited antimicrobial activity, primarily against Gram-positive bacteria. PCR and sequencing analysis revealed the presence of lantibiotic-like encoding genes in these strains.
FRONTIERS IN MICROBIOLOGY
(2023)
Review
Agriculture, Dairy & Animal Science
Jessica Ribeiro, Vanessa Silva, Andreia Monteiro, Madalena Vieira-Pinto, Gilberto Igrejas, Filipa S. Reis, Lillian Barros, Patricia Poeta
Summary: Chicken meat is widely consumed around the world, and antibiotics have been used in its production to ensure high levels of output. However, the use of antibiotics in animal production has led to the emergence of antibiotic-resistant bacteria, particularly in the gut microbiota. Studies have found resistant strains of Enterococcus spp. and Escherichia coli in the gastrointestinal tract of chickens, with Enterococcus spp. showing resistance to at least seven classes of antibiotics and E. coli showing resistance to at least four. Some clonal lineages have also been identified in both broilers and humans, suggesting the transmission of bacteria through contaminated food, direct contact with animals, or environmental exposure.
Article
Agriculture, Dairy & Animal Science
Sandra Cunha, Carla Miranda, Angela Martins, Ruben Soares, Manuel Maia, Filipe Silva, Gilberto Igrejas, Patricia Poeta
Summary: This study investigated whether bovine colostrum can serve as a reservoir and vehicle for the transmission of antibiotic-resistant Enterococcus spp. The results showed the presence of antibiotic-resistant species and genes in calf colostrum, with a high percentage of multidrug-resistant isolates. This highlights the importance of hygiene measures to reduce microbial contamination of colostrum.
Article
Agriculture, Dairy & Animal Science
Ruben Soares, Carla Miranda, Sandra Cunha, Luis Ferreira, Angela Martins, Gilberto Igrejas, Patricia Poeta
Summary: The aim of this study was to assess the presence of Enterococcus species in ornamental animal feed and characterize their antimicrobial resistance and virulence factors. A total of 103 Enterococcus isolates were recovered from 57 samples of ornamental animal feed, with Enterococcus faecalis and E. faecium being the most frequently identified species. The isolates exhibited high resistance to rifampicin and erythromycin, and many were classified as multidrug-resistant. These findings highlight the public health concern of antibiotic-resistant Enterococcus spp. in ornamental animal feed and the increasing interaction between these animals and humans.
Article
Microbiology
Telma de Sousa, Michel Hebraud, Olimpia Alves, Eliana Costa, Luis Maltez, Jose Eduardo Pereira, Angela Martins, Gilberto Igrejas, Patricia Poeta
Summary: A study conducted at the Medical Center of Tras-os-Montes and Alto Douro (CHTMAD) found that Pseudomonas aeruginosa strains causing urinary tract infections are resistant to multiple antibiotics and have high biofilm formation and motility capabilities, posing a serious public health problem.
Article
Microbiology
Rosa Fernandez-Fernandez, Carmen Lozano, Rine Christopher Reuben, Laura Ruiz-Ripa, Myriam Zarazaga, Carmen Torres
Summary: Novel approaches to combat antimicrobial resistance (AMR) are needed, and antimicrobial peptides, particularly bacteriocins, have gained attention as potential alternatives to antibiotics. Bacteriocins produced by Staphylococcus, known as staphylococcins, have shown significant antimicrobial potential and are considered promising candidates to combat AMR. Additionally, various bacteriocin-producing Staphylococcus strains, including coagulase-negative staphylococci (CoNS), have been identified as alternative options.
Article
Infectious Diseases
Vanessa Silva, Adriana Silva, Jessica Ribeiro, Alfredo Aires, Rosa Carvalho, Joana S. Amaral, Lillian Barros, Gilberto Igrejas, Patricia Poeta
Summary: Antimicrobial resistance is a growing global public health problem, and there is a need for new antimicrobial agents. The food industry produces bioactive by-products rich in phenolic compounds, which have antioxidant and antimicrobial properties. This study aimed to characterize the phenolic compounds in pomegranate, quince, and persimmon by-products and assess their antioxidant and antimicrobial activities.
Article
Environmental Sciences
Angela Barreto, Joana Santos, Vania Calisto, Luciana S. Rocha, Vera L. Maria
Summary: Nanoplastics are widespread in the environment and can increase the toxicity of other contaminants, as shown in this study with zebrafish embryos. The mixture of polystyrene nanoparticles and diphenhydramine had synergistic toxic effects, leading to high mortality, malformations, and decreased heartbeat rates.
Article
Infectious Diseases
Sandra Martinez-Alvarez, Pierre Chatre, Teresa Cardona-Cabrera, Pauline Francois, Alberto Sanchez-Cano, Ursula Hofle, Myriam Zarazaga, Jean-Yves Madec, Marisa Haenni, Carmen Torres
Summary: This study aimed to characterize Escherichia coli isolates from cloacal samples of white stork nestlings, with a special focus on extended-spectrum β-lactamases (ESBLs)-producing E. coli isolates and their plasmid content. Various methods were used, including disc diffusion method, polymerase chain reaction (PCR), Pulsed-Field-Gel-Electrophoresis (PFGE), Southern blotting, and whole-genome sequencing, to screen for antimicrobial susceptibility, genotypic characterization, and plasmid sequencing. The study identified the presence of ESBL-producing E. coli and their plasmid characteristics.
JOURNAL OF GLOBAL ANTIMICROBIAL RESISTANCE
(2023)
Article
Biotechnology & Applied Microbiology
Rosa Fernandez-Fernandez, Ahmed M. A. Elsherbini, Carmen Lozano, Agusti Martinez, Maria de Toro, Myriam Zarazaga, Andreas Peschel, Bernhard Krismer, Carmen Torres
Summary: This study investigated the presence of bacteriocin gene clusters among 22 commensal Staphylococcus isolates from different origins. Five types of bacteriocin gene clusters were detected, including lanthipeptides, circular bacteriocins, lugdunin, and micrococcin P1.
PROBIOTICS AND ANTIMICROBIAL PROTEINS
(2023)
Article
Pharmacology & Pharmacy
Luis Filipe, Telma de Sousa, Dario Silva, Miguel M. Santos, Manuela Ribeiro Carrott, Patricia Poeta, Luis C. Branco, Sandra Gago
Summary: The combination of active pharmaceutical ingredients in the form of ionic liquids or organic salts (API-OSILs) with mesoporous silica nanoparticles (MSNs) as drug carriers can enhance the capabilities of current antibiotics, especially against resistant strains of bacteria. In this study, three nanomaterials based on the modification of MSN surface with different ionic liquids coupled with anionic ciprofloxacin were prepared. These materials showed no cytotoxicity and exhibited improved antibacterial activity against both resistant and sensitive strains of K. pneumoniae. One particular nanomaterial, [MSN-Chol][Cip], showed significant enhancement with a tenfold decrease in MIC against sensitive strains. The importance of this study is rated at 8 out of 10 for its potential application in combating antibiotic resistance.
Article
Environmental Sciences
Angela Barreto, Joana Santos, Goncalo Andrade, Matilde Santos, Vera L. Maria
Summary: Despite the growing concern over nanoplastics' environmental impacts, this study found that polystyrene nanoparticles had no significant effects on the survival and reproduction of the soil-dwelling species Folsomia candida. However, oxidative damage was detected in the offspring of the first generation. These findings emphasize the importance of evaluating multigenerational effects to fully understand the long-term impact of contaminants like nanoplastics.
