Directed evolution of a cellobiose utilization pathway in Saccharomyces cerevisiae by simultaneously engineering multiple proteins

Feasibility of xylose fermentation by engineeredSaccharomyces cerevisiaeoverexpressing endogenous aldose reductase (GRE3), xylitol dehydrogenase (XYL2), and xylulokinase (XYL3) fromScheffersomyces stipitis

(2013) Soo Rin Kim et al.   FEMS YEAST RESEARCH

Combinatorial Design of a Highly Efficient Xylose-Utilizing Pathway in Saccharomyces cerevisiae for the Production of Cellulosic Biofuels

(2012) Byoungjin Kim et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

Co-fermentation of xylose and cellobiose by an engineered Saccharomyces cerevisiae

(2012) Kimberly A. Aeling et al.   JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY

A molecular transporter engineering approach to improving xylose catabolism in Saccharomyces cerevisiae

(2012) Eric M. Young et al.   METABOLIC ENGINEERING

Customized optimization of metabolic pathways by combinatorial transcriptional engineering

(2012) Jing Du et al.   NUCLEIC ACIDS RESEARCH

Mutations in the substrate entrance region of  -glucosidase from Trichoderma reesei improve enzyme activity and thermostability

(2012) H.-L. Lee et al.   PROTEIN ENGINEERING DESIGN & SELECTION

Driving Forces Enable High-Titer Anaerobic 1-Butanol Synthesis in Escherichia coli

(2011) Claire R. Shen et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

Co-expression of a cellobiose phosphorylase and lactose permease enables intracellular cellobiose utilisation by Saccharomyces cerevisiae

(2011) Christa J. Sadie et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Development of an industrial ethanol-producing yeast strain for efficient utilization of cellobiose

(2011) Zhong-peng Guo et al.   ENZYME AND MICROBIAL TECHNOLOGY

Enzyme mechanism as a kinetic control element for designing synthetic biofuel pathways

(2011) Brooks B Bond-Watts et al.   Nature Chemical Biology

Reiterative Recombination for the in vivo assembly of libraries of multigene pathways

(2011) L. M. Wingler et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Co-fermentation of cellobiose and xylose using beta-glucosidase displaying diploid industrial yeast strain OC-2

(2010) Satoshi Saitoh et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Single-step linker-based combinatorial assembly of promoter and gene cassettes for pathway engineering

(2010) Adi Ramon et al.   BIOTECHNOLOGY LETTERS

Engineered microbial systems for enhanced conversion of lignocellulosic biomass

(2010) James G Elkins et al.   CURRENT OPINION IN BIOTECHNOLOGY

Structural and functional analysis of three β-glucosidases from bacterium Clostridium cellulovorans, fungus Trichoderma reesei and termite Neotermes koshunensis

(2010) Wen-Yih Jeng et al.   JOURNAL OF STRUCTURAL BIOLOGY

Rapid dissection of a complex phenotype through genomic-scale mapping of fitness altering genes

(2010) T.E. Warnecke et al.   METABOLIC ENGINEERING

Overcoming glucose repression in mixed sugar fermentation by co-expressing a cellobiose transporter and a β-glucosidase in Saccharomyces cerevisiae

(2010) Sijin Li et al.   Molecular BioSystems

Rapid profiling of a microbial genome using mixtures of barcoded oligonucleotides

(2010) Joseph R Warner et al.   NATURE BIOTECHNOLOGY

Engineered Saccharomyces cerevisiae capable of simultaneous cellobiose and xylose fermentation

(2010) S.-J. Ha et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Cellodextrin Transport in Yeast for Improved Biofuel Production

(2010) J. M. Galazka et al.   SCIENCE

Structural insights into the substrate recognition properties of β-glucosidase

(2009) Ki Hyun Nam et al.   BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS

An evolved xylose transporter from Zymomonas mobilis enhances sugar transport in Escherichia coli

(2009) Chuan Ren et al.   Microbial Cell Factories

Programming cells by multiplex genome engineering and accelerated evolution

(2009) Harris H. Wang et al.   NATURE

Synthetic protein scaffolds provide modular control over metabolic flux

(2009) John E Dueber et al.   NATURE BIOTECHNOLOGY

Automated design of synthetic ribosome binding sites to control protein expression

(2009) Howard M Salis et al.   NATURE BIOTECHNOLOGY

Microarray labeling extension values: laboratory signatures for Affymetrix GeneChips

(2009) Yun-Shien Lee et al.   NUCLEIC ACIDS RESEARCH

Circular Polymerase Extension Cloning of Complex Gene Libraries and Pathways

(2009) Jiayuan Quan et al.   PLoS One

Redesigning Enzymes Based on Adaptive Evolution for Optimal Function in Synthetic Metabolic Pathways

(2008) Yasuo Yoshikuni et al.   CHEMISTRY & BIOLOGY

Combinatorial pathway analysis for improved L-tyrosine production in Escherichia coli: Identification of enzymatic bottlenecks by systematic gene overexpression

(2008) Tina Lütke-Eversloh et al.   METABOLIC ENGINEERING

Optimization of the mevalonate-based isoprenoid biosynthetic pathway in Escherichia coli for production of the anti-malarial drug precursor amorpha-4,11-diene

(2008) Jennifer R. Anthony et al.   METABOLIC ENGINEERING