期刊
MICROBIAL CELL FACTORIES
卷 11, 期 -, 页码 -出版社
BMC
DOI: 10.1186/1475-2859-11-51
关键词
Conjugated linoleic acid; Linoleate isomerase; Codon optimization; Multi-copy integration; Propionibacterium acnes; Yarrowia lipolytica
资金
- National Science Fund for Distinguished Young Scholars [31125021]
- National Natural Science Foundation of China [31171636, 81071685, 20836003]
- National Basic Research Program of China 973 Program [2012CB720802, B07029]
- Fundamental Research Funds for the Central Universities [JUSRP 11017, JUSRP31002, JUSRP31103]
- Preparatory Research Foundation of Jiangnan University, China [2009LYY016, SKLF-TS-201101, SKLF-MB-200802]
Background: Conjugated linoleic acid (CLA) has many well-documented beneficial physiological effects. Due to the insufficient natural supply of CLA and low specificity of chemically produced CLA, an effective and isomer-specific production process is required for medicinal and nutritional purposes. Results: The linoleic acid isomerase gene from Propionibacterium acnes was expressed in Yarrowia lipolytica Polh. Codon usage optimization of the PAI and multi-copy integration significantly improved the expression level of PAI in Y. lipolytica. The percentage of trans-10, cis-12 CLA was six times higher in yeast carrying the codon-optimized gene than in yeast carrying the native gene. In combination with multi-copy integration, the production yield was raised to approximately 30-fold. The amount of trans-10, cis-12 CLA reached 5.9% of total fatty acid yield in transformed Y. lipolytica. Conclusions: This is the first report of production of trans-10, cis-12 CLA by the oleaginous yeast Y. lipolytica, using glucose as the sole carbon source through expression of linoleic acid isomerase from Propionibacterium acnes.
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