Article
Cell Biology
Hao Yin, Jiaoli Zhu, Yi Jiang, Yijing Mao, Chenquan Tang, Hui Cao, Yufang Huang, Huijun Zhu, Jianping Luo, Qingjiang Jin, Qinglei Jin, Yi Xue, Xin Wang
Summary: Urinary tract infections (UTIs) are a common infectious disease, with increasing drug resistance of Escherichia coli (E. coli). Shionone, a potential therapeutic agent, was evaluated in rat and cell models of UTI. Treatment with Shionone resulted in decreased bladder weight, reduced inflammation, and lowered levels of proinflammatory factors in both models. Immunofluorescence analysis showed a decrease in bacterial load, indicating the effectiveness of Shionone in treating UTI. These findings provide a basis for further research in UTI treatment.
CELLULAR MICROBIOLOGY
(2023)
Article
Energy & Fuels
Balasubramani Ramprakash, Aran Incharoensakdi
Summary: The study investigated the conversion of garden wastes into hydrogen using different pretreatment and hydrolysis methods, finding that the optimal conditions for maximum hydrogen production were 2% sulfuric acid and 2% Viscozyme L. The fermentation by Escherichia coli of hydrolyzate from combined acid and enzyme treatment resulted in a 2.7-fold increase in hydrogen production compared to untreated garden wastes.
Article
Biotechnology & Applied Microbiology
Jianfeng Mei, Xia Wu, Sujing Zheng, Yu Yi, Xudong Wang, Guoqing Ying
Summary: Bilirubin, a valuable Chinese medicine, is currently produced from pig bile, but due to raw material shortage, its price has increased. This study explores the use of biotransformation to produce bilirubin using recombinant E. coli expressing biliverdin reductase. With optimized conditions, the conversion yield reached 72.3%. This provides a potential alternative process for bilirubin production.
BIOPROCESS AND BIOSYSTEMS ENGINEERING
(2022)
Article
Chemistry, Multidisciplinary
Xi Luo, Yifeng Wang, Weilong Zheng, Xiaolong Sun, Gaowei Hu, Longfei Yin, Yingying Zhang, Fengwei Yin, Yongqian Fu
Summary: Computer-aided rational design was used to improve the thermostability and catalytic activity of LrLDH, providing a reference for the modification of other enzymes.
Article
Multidisciplinary Sciences
Jonathan C. Greenhalgh, Sarah A. Fahlberg, Brian F. Pfleger, Philip A. Romero
Summary: This study utilized a machine learning-driven approach to engineer fatty acyl reductases (FARs) with enhanced activity on acyl-ACP substrates for increased production of fatty alcohols. Through iterative design and testing, the engineered enzymes produced over twofold more fatty alcohols than the starting natural sequences. The top sequence identified had an enhanced catalytic rate on palmitoyl-ACP, demonstrating the power of machine learning in protein engineering for challenging proteins.
NATURE COMMUNICATIONS
(2021)
Article
Chemistry, Multidisciplinary
Micjel Chavez Morejon, Alexander Franz, Rohan Karande, Falk Harnisch
Summary: We demonstrate a method for synthesizing adipic acid (AA), a key component of polyamides like Nylon-6,6, from lignin-derived feedstock using a combination of electrochemical hydrogenation and biotransformation. The electrochemical hydrogenation process achieves a 68% yield of cyclohexanol from phenol at a 69% coulombic efficiency, and the electrochemical hydrogenation of an aromatic mixture obtained from lignin depolymerization yields up to 83%. By further biotransformation with recombinant Pseudomonas taiwanensis VLB120, AA is produced at a rate of 0.02 g L-1 h(-1) with a yield of up to 61% in 5 hours.
Article
Chemistry, Multidisciplinary
Dongsoo Yang, Woo Dae Jang, Sang Yup Lee
Summary: Researchers have successfully biosynthesized carminic acid from glucose in engineered Escherichia coli. Through enzyme screening and optimization, the biosynthesis of carminic acid was achieved.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2021)
Article
Agricultural Engineering
Suman Lama, Yeonhee Kim, Dat Tuan Nguyen, Chae Ho Im, Mugesh Sankaranarayanan, Sunghoon Park
Summary: The study explores a two-stage cultivation strategy for improving 3-HP production from acetate by optimizing cellular metabolic pathways, particularly through the modulation of the GS pathway to enhance 3-HP yield.
BIORESOURCE TECHNOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Yi Yan, Yajun Bai, Xiaohui Zheng, Yujie Cai
Summary: Due to its strong antioxidant capacity, hydroxytyrosol (HT) has a long history of use in cosmetics and food industries. However, current methods of isolating HT are not optimal, which leads to the need for more efficient synthesis methods. This study aimed to create an Escherichia coli (E. coli) whole cell system capable of synthesizing HT from dopamine by constructing a custom biosynthetic pathway. Through overexpression of enzymes and identifying rate-limiting enzymes, the synthesis of HT was significantly boosted. This study establishes a biocatalytic pathway for the synthesis of new building blocks for industrially high-performance HT.
BIOCHEMICAL ENGINEERING JOURNAL
(2023)
Review
Biochemical Research Methods
Shuobo Shi, Nailing Qi, Jens Nielsen
Summary: Microorganisms have become an attractive option for producing various chemicals from renewable resources. The CRISPR-Cas system has emerged as a powerful tool for manipulating nucleic acids and generating cell factories with desired properties. This review highlights recent advances in using CRISPR-Cas systems to develop microbial cell factories for chemical production, including identifying better biocatalysts, fine-tuning metabolic flux, utilizing inexpensive substrates, and improving metabolic robustness. The potential applications and further development of CRISPR-Cas systems for strain improvement are also discussed.
CURRENT OPINION IN BIOTECHNOLOGY
(2022)
Article
Multidisciplinary Sciences
Dae-yeol Ye, Myung Hyun Noh, Jo Hyun Moon, Alfonsina Milito, Minsun Kim, Jeong Wook Lee, Jae-Seong Yang, Gyoo Yeol Jung
Summary: Unlike eukaryotic systems, bacterial hosts lack a membranous system, which is a limitation for efficient metabolic engineering. In this study, a kinetic compartmentalization strategy was used to increase substrate availability from competitive reactions for the efficient production of itaconate in E. coli.
NATURE COMMUNICATIONS
(2022)
Article
Chemistry, Multidisciplinary
Sakuntala Mutyala, Shuwei Li, Himanshu Khandelwal, Da Seul Kong, Jung Rae Kim
Summary: This study found that acetate can be converted to succinate under microaerobic conditions by overexpressing citrate synthase (gltA) in Pseudomonas putida. The gltA-KT strain showed a nearly 50% improvement in succinate production compared to the wild type. Under optimal pH of 7.5, the accumulation of succinate was approximately 400% higher than that of the wild type. Overall, gltA overexpression alone resulted in 9.5% of the maximum theoretical yield in a minimal medium with acetate as the sole carbon source. This highlights the importance of citrate synthase in acetate conversion to succinate by P. putida under microaerobic conditions.
Article
Pharmacology & Pharmacy
Thais Barros, Larissa Brumano, Marcela Freitas, Adalberto Pessoa, Nadia Parachin, Perola O. Magalhaes
Summary: This study aimed to produce L-asparaginase from Escherichia coli for the treatment of acute lymphocytic leukemia. The defined medium showed the highest enzyme activity. Batch and fed-batch cultures were conducted to achieve high cell density and increase enzyme productivity through lactose induction.
Review
Biotechnology & Applied Microbiology
Peng Zhao, Pingfang Tian
Summary: This review summarizes key factors in bacterial production of 3-HP, including host strains, metabolic pathways, key enzymes, and challenges in achieving high yields.
WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY
(2021)
Article
Agriculture, Multidisciplinary
Qiang Guo, Ling-Jie Zheng, Xuan Luo, Xin-Quan Gao, Chen-Yang Liu, Li Deng, Li-Hai Fan, Hui-Dong Zheng
Summary: This study proposed a new method to efficiently produce D-allulose through fermentation in Escherichia coli using metabolic engineering techniques, achieving a high yield of D-allulose.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2021)
Article
Biotechnology & Applied Microbiology
John W. K. Oliver, Shota Atsumi
METABOLIC ENGINEERING
(2015)
Review
Biochemical Research Methods
Austin L. Carroll, Shuchi H. Desai, Shota Atsumi
CURRENT OPINION IN BIOTECHNOLOGY
(2016)
Review
Biochemistry & Molecular Biology
Neal J. Oliver, Christine A. Rabinovitch-Deere, Austin L. Carroll, Nicole E. Nozzi, Anna E. Case, Shota Atsumi
CURRENT OPINION IN CHEMICAL BIOLOGY
(2016)
Review
Biotechnology & Applied Microbiology
Anna E. Case, Shota Atsumi
JOURNAL OF BIOTECHNOLOGY
(2016)
Article
Multidisciplinary Sciences
Masahiro Kanno, Austin L. Carroll, Shota Atsumi
NATURE COMMUNICATIONS
(2017)
Article
Biochemical Research Methods
Nicole E. Nozzi, Anna E. Case, Austin L. Carroll, Shota Atsumi
ACS SYNTHETIC BIOLOGY
(2017)
Review
Biochemical Research Methods
Morgan M. Matson, Shota Atsumi
CURRENT OPINION IN BIOTECHNOLOGY
(2018)
Article
Biotechnology & Applied Microbiology
John W. K. Oliver, Iara M. P. Machado, Hisanari Yoneda, Shota Atsumi
METABOLIC ENGINEERING
(2014)
Article
Biochemistry & Molecular Biology
Gabriel M. Rodriguez, Yohei Tashiro, Shota Atsumi
NATURE CHEMICAL BIOLOGY
(2014)
Article
Biochemistry & Molecular Biology
Jake N. Gonzales, Morgan M. Matson, Shota Atsumi
Article
Biotechnology & Applied Microbiology
Morgan M. Matson, Mateo M. Cepeda, Angela Zhang, Anna E. Case, Erol S. Kavvas, Xiaokang Wang, Austin L. Carroll, Ilias Tagkopoulos, Shota Atsumi
Summary: Through adaptive laboratory evolution, E. coli was improved for enhanced tolerance and production of isobutyl acetate (IBA). Mutants with increased acetyl-CoA supply and altered transcriptional machinery were isolated, resulting in a strain capable of producing 3.2-fold more IBA than the parent strain.
METABOLIC ENGINEERING
(2022)
Article
Biotechnology & Applied Microbiology
Shunichi Kobayashi, Shota Atsumi, Kazunori Ikebukuro, Koji Sode, Ryutaro Asano
Summary: In this study, a green light-inducible alcohol production system was successfully integrated into cyanobacteria to produce value-added chemicals without using expensive chemical inducers. This eco-friendly and cost-effective approach increases the feasibility of cyanobacterial bioprocesses.
MICROBIAL CELL FACTORIES
(2022)
Article
Biotechnology & Applied Microbiology
Tanner R. Treece, Santanu Pattanayak, Morgan M. Matson, Mateo M. Cepeda, Louise A. Berben, Shota Atsumi
Summary: In this study, we developed an electrical-biological hybrid system that utilizes an engineered microorganism to consume formate produced electrocatalytically, thereby enhancing the bioproduction of isobutanol. By integrating the reductive glycine pathway and the isobutanol biosynthetic pathway into Escherichia coli, we achieved a strain capable of consuming formate and producing isobutanol at a yield exceeding the theoretical maximum from glucose. Our findings demonstrate that electrocatalytically reduced CO2 can enhance chemical production in E. coli and highlight the potential of engineering E. coli for carbon-efficient methods of chemical production.
METABOLIC ENGINEERING
(2023)
Article
Biology
Jake N. Gonzales, Tanner R. Treece, Stephen P. Mayfield, Ryan Simkovsky, Shota Atsumi
Summary: This study investigates the potential of using the photoautotroph cyanobacterium Synechococcus elongatus PCC 7942 as a chassis organism for lignocellulosic chemical production. By modifying the cyanobacterium to import monosaccharides, it can grow well at high lysate concentrations and enhance carbon efficiency by fixing CO2. This study is important in enabling industrial scale production of commodity chemicals from a combination of sunlight, CO2, and lignocellulosic sugars.
COMMUNICATIONS BIOLOGY
(2023)
Article
Food Science & Technology
Jayce E. Taylor, Dileep Sai Kumar Palur, Angela Zhang, Jake N. Gonzales, Augustine Arredondo, Timothy A. Coulther, Amiruddin Bin Johan Lechner, Elys P. Rodriguez, Oliver Fiehn, John Didzbalis, Justin B. Siegel, Shota Atsumi
Summary: Due to the rise in obesity and diabetes, consumers are searching for ways to reduce sugar intake without sacrificing taste. D-psicose is a promising sugar substitute with perfect palatability. However, current methods of production are inefficient and costly, limiting accessibility. In this study, researchers discovered a thermodynamically favorable pathway for D-psicose production in E. coli. Through genetic modifications and dynamic regulation, they successfully produced D-psicose with high yield and viability. These results show the potential of whole-cell catalysis as a sustainable alternative to current production methods.
NPJ SCIENCE OF FOOD
(2023)