期刊
MICROBES AND INFECTION
卷 13, 期 2, 页码 179-188出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.micinf.2010.10.017
关键词
Severe acute respiratory syndrome coronavirus (SARS-CoV); Accessory proteins; 8b protein; Envelope (E); Ubiquitin-independent; Proteasome pathway
资金
- Agency for Science, Technology and Research (A*STAR), Singapore
The severe acute respiratory syndrome coronavirus (SARS-CoV) 8b protein, which is not expressed by other known coronaviruses, can down-regulate the envelope (E) protein via a proteasome-dependent pathway. Here, we showed that the down-regulation of E is not dependent on the lysine residues on 8b and the reduction of polyubiquitination of E mutants is not correlated with their down-regulation by 8b, suggesting an ubiquitin-independent proteasome pathway is involved. A time-course study revealed that 8b was expressed at late-stages of SARS-CoV infection. By using Vero E6 cells stably expressing green fluorescence protein-tagged 8b, ectopic expression of 8b was shown to significantly reduce the production of progeny virus and down-regulate E expression. Taken together, these results suggest that 8b negatively modulates virus replication by down-regulating E via an ubiquitin-independent proteasome pathway. (C) 2010 Institut Pasteur. Published by Elsevier Masson SAS. All rights reserved.
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