Article
Cell & Tissue Engineering
Panagiota Kakni, Barry Jutten, Daniel Teixeira Oliveira Carvalho, John Penders, Roman Truckenmuller, Pamela Habibovic, Stefan Giselbrecht
Summary: The gut microbiome is crucial for proper gut function, but imbalances can lead to gastrointestinal conditions. Current models do not accurately depict in vivo situations, thus more advanced in vitro models are needed to study host-microbiome interactions. Here, we developed a novel hypoxia-tolerant apical-out small intestinal organoid model that mimics intestinal cell composition and structure. Co-cultures with anaerobic strains showed successful colonization and probiotic benefits. These organoids will help unravel unknown mechanisms and aid in the development of microbiome-related therapeutics.
JOURNAL OF TISSUE ENGINEERING
(2023)
Article
Microbiology
Fauzy Nasher, Burhan Lehri, Megan F. Horney, Richard A. Stabler, Brendan W. Wren
Summary: The study reveals a new host-pathogen interaction between Campylobacter jejuni and Acanthamoebae, confirming that amoebae play a crucial role in the persistence, adaptability, and potential transmission of C. jejuni.
Review
Biotechnology & Applied Microbiology
Leon Kemper, Andreas Hensel
Summary: Campylobacter jejuni, causing severe enteritis, has unique features and adapts effectively to environmental conditions. The review summarizes the strategies of C. jejuni for host-pathogen interaction, providing insights for future drug development.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2023)
Review
Biochemistry & Molecular Biology
Asahiro Morishita, Kyoko Oura, Tomoko Tadokoro, Koji Fujita, Joji Tani, Tsutomu Masaki
Summary: The liver is a non-immunological organ involved in metabolic activities, nutrient storage, and detoxification. Resident immune cells in the liver drive immunological reactions and certain microRNAs are associated with the immune response to pathogens, although the exact mechanism of miRNA interference in these interactions remains unclear.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Immunology
Joshua Oluoch Amimo, Sergei Alekseevich Raev, Juliet Chepngeno, Alfred Omwando Mainga, Yusheng Guo, Linda Saif, Anastasia N. Vlasova
Summary: Rotavirus is a major pathogen causing severe diarrheal illness in young children and animals worldwide, infecting mature enterocytes in the intestinal epithelium. The immune system of intestinal epithelial cells plays a crucial role in countering rotavirus invasion, while rotavirus has evolved mechanisms to evade host immunity. Understanding the mechanisms of rotavirus-IECs interactions is essential for developing strategies to control infections.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Immunology
David Smith, Daniel R. G. Price, Alison Burrells, Marc N. Faber, Katie A. Hildersley, Cosmin Chintoan-Uta, Ambre F. Chapuis, Mark Stevens, Karen Stevenson, Stewart T. G. Burgess, Elisabeth A. Innes, Alasdair J. Nisbet, Tom N. McNeilly
Summary: The development of ovine epithelial organoids provides a valuable tool for studying interactions between pathogens and epithelial cells in sheep gastrointestinal infections. These organoids exhibit stable transcript profiles and can be cryopreserved, reducing the need for animal experimentation. Furthermore, they can be used for pathogen co-culture experiments, showcasing their potential utility in pathogen research.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2021)
Review
Gastroenterology & Hepatology
Fei Gao, Hengwei Wu, Limengmeng Wang, Yanmin Zhao, He Huang
Summary: Despite advancements in HSCT, GVHD, especially intestinal GVHD, still poses a significant challenge to the procedure. Traditionally, GVHD has been seen as an immune response, with the intestine as a target. However, factors such as altered intestinal microbiome and epithelial damage contribute to intestinal damage after transplantation. This review summarizes these factors and explores the potential of remodeling intestinal homeostasis in GVHD management.
Article
Pharmacology & Pharmacy
Maria Garcia-Diaz, Maria del Mar Cendra, Raquel Alonso-Roman, Maria Urdaniz, Eduard Torrents, Elena Martinez
Summary: The intestinal mucus acts as a barrier on the luminal surface of the intestinal epithelium, providing defense against external insults and supporting the resident microbiota. In a 3D villus-like hydrogel scaffold model, the secreted mucus enhanced adhesion of a pathogenic E. coli strain while also protecting against invasion.
Review
Biotechnology & Applied Microbiology
Paula M. Tribelli, Nancy Lopez
Summary: This review provides an overview of temperature-response mechanisms in Pseudomonas species and highlights their relevance to human and animal health as well as agricultural applications.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2022)
Review
Microbiology
Mehreen Anjum, Arja Laitila, Arthur C. Ouwehand, Sofia D. Forssten
Summary: There are various models available to study the interaction between probiotics and pathogens in the gastrointestinal tract, with in vitro methods being faster, cost-effective, and ethically superior. The selection of the optimal model depends on the research question.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Immunology
Ruby White, Frances Blow, Amy H. Buck, Maria A. Duque-Correa
Summary: This study outlines the applications and important considerations of using organoids to study gastrointestinal nematode development and interactions with their hosts. By carefully using different organoid culture configurations, a closer replication of the in vivo infection context can be achieved, leading to new knowledge on gastrointestinal nematode infection biology and the development of valuable experimental tools.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Review
Cell Biology
Johannes Westman, Sergio Grinstein
Summary: The ability of phagosomes to halt microbial growth is closely linked to their ability to acidify their luminal pH, but certain pathogens can survive and replicate inside phagosomes by targeting the pH-regulatory machinery of host cells to survive or escape.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Review
Cell Biology
Julie Bas, Philippe Jay, Francois Gerbe
Summary: The intestinal epithelium plays a vital role in maintaining gut homeostasis by acting as a barrier and controlling the activation of the immune system. Tuft cells, a unique epithelial cell lineage, were recently discovered to initiate type 2 immune responses and recognize various luminal cues in the gut. They have emerged as sentinel cells in the host's interaction with pathogens and have the potential to impact gut physiopathology. This review focuses on tuft cells, their functions, and their potential impact on diseases.
SEMINARS IN CELL & DEVELOPMENTAL BIOLOGY
(2023)
Article
Cell Biology
Constantina Neophytou, Chrysoula Pitsouli
Summary: Diet plays a crucial role in regulating metabolism and interacting with the intestinal microbiome. This study focuses on the role of the Drosophila intestinal stem cell-specific biotin transporter, Smvt, in maintaining gut homeostasis, infection-induced regeneration, and tumorigenesis. The findings suggest that biotin transported by Smvt is essential for stem cell mitosis and intestinal maintenance. Dysbiosis and increased pathogen growth were observed in flies with Smvt deficiency, while commensal bacteria that produce biotin can rescue reduced stem cell mitosis. Additionally, both Smvt and commensals play a role in controlling intestinal tumor growth.
Review
Microbiology
Vera Vozandychova, Pavla Stojkova, Kamil Hercik, Pavel Rehulka, Jiri Stulik
Summary: Ubiquitination, similar to phosphorylation and acetylation, plays a crucial role in regulating various cell processes. Understanding how pathogens manipulate host ubiquitination processes is important for vaccine development and disease treatment. Pathogenic bacteria encode effector proteins targeting the host ubiquitin machinery to disrupt host defense processes.
Article
Biotechnology & Applied Microbiology
Amira Shousha, Nattakarn Awaiwanont, Dmitrij Sofka, Frans J. M. Smulders, Peter Paulsen, Michael P. Szostak, Tom Humphrey, Friederike Hilbert
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2015)
Article
Microbiology
Ivana Indikova, Tom J. Humphrey, Friederike Hilbert
FRONTIERS IN MICROBIOLOGY
(2015)
Article
Multidisciplinary Sciences
Sakaoporn Prachantasena, Petcharatt Charununtakorn, Suthida Muangnoicharoen, Luck Hankla, Natthaporn Techawal, Prapansak Chaveerach, Pravate Tuitemwong, Nipa Chokesajjawatee, Nicola Williams, Tom Humphrey, Taradon Luangtongkum
Article
Biotechnology & Applied Microbiology
Gemma Chaloner, Paul Wigley, Suzanne Humphrey, Kirsty Kemmett, Lizeth Lacharme-Lora, Tom Humphrey, Nicola Williams
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2014)
Article
Veterinary Sciences
L. K. Williams, L. C. Sait, E. K. Trantham, T. A. Cogan, T. J. Humphrey
Article
Veterinary Sciences
B. N. Parsons, G. Crayford, T. J. Humphrey, P. Wigley
Article
Veterinary Sciences
K. Kemmett, N. J. Williams, G. Chaloner, S. Humphrey, P. Wigley, T. Humphrey
Article
Microbiology
Sree V. Aroori, Tristan A. Cogan, Tom J. Humphrey
CURRENT MICROBIOLOGY
(2013)
Article
Microbiology
Chris Coward, Leanne Sait, Tristan Cogan, Tom J. Humphrey, Duncan J. Maskell
FEMS MICROBIOLOGY LETTERS
(2013)
Article
Microbiology
Georgina Crayford, Janine L. Coombes, Tom J. Humphrey, Paul Wigley
Article
Multidisciplinary Sciences
Kirsty Kemmett, Tom Humphrey, Steven Rushton, Andrew Close, Paul Wigley, Nicola J. Williams
Article
Multidisciplinary Sciences
Suzanne Humphrey, Lizeth Lacharme-Lora, Gemma Chaloner, Kirsty Gibbs, Tom Humphrey, Nicola Williams, Paul Wigley
Article
Multidisciplinary Sciences
Gemma C. Langridge, Maria Fookes, Thomas R. Connor, Theresa Feltwell, Nicholas Feasey, Bryony N. Parsons, Helena M. B. Seth-Smith, Lars Barquist, Anna Stedman, Tom Humphrey, Paul Wigley, Sarah E. Peters, Duncan J. Maskell, Jukka Corander, Jose A. Chabalgoity, Paul Barrow, Julian Parkhill, Gordon Dougan, Nicholas R. Thomson
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2015)
Article
Microbiology
Robert A. Kingsley, Sally Kay, Thomas Connor, Lars Barquist, Leanne Sait, Kathryn E. Holt, Karthi Sivaraman, Thomas Wileman, David Goulding, Simon Clare, Christine Hale, Aswin Seshasayee, Simon Harris, Nicholas R. Thomson, Paul Gardner, Wolfgang Rabsch, Paul Wigley, Tom Humphrey, Julian Parkhill, Gordon Dougan
Article
Microbiology
Suzanne Humphrey, Gemma Chaloner, Kirsty Kemmett, Nicola Davidson, Nicola Williams, Anja Kipar, Tom Humphrey, Paul Wigley