期刊
MICROBES AND INFECTION
卷 11, 期 6-7, 页码 654-660出版社
ELSEVIER
DOI: 10.1016/j.micinf.2009.03.003
关键词
H5N1; Single-dose; Vaccine delivery system
资金
- National Health Research Institutes [94A1-VCPP01-002]
- Taiwan Centers for Disease Control, Taiwan Pandemic Influenza Vaccine Research and Development Program [96A1-VCSP36-019]
Vaccine shortages are a major obstacle to influenza pandemic preparedness. Increasing vaccine efficiency provides a potentially effective way to overcome this problem. Specifically, using single-dose immunization to induce protective immunity is an attractive approach to emergency/massive vaccination. In this report, we propose a novel nanoemulsion comprised of the bioresorbable polymer, Span (R) 85, and squalene forming a ready-to-use adjuvant, called PELC. After formulation with PELC, inactivated H5N1 virus was intramuscularly administered to mice via a single injection. The data demonstrate that inactivated virus containing 0.5 mu g hemagglutinin (HA) and formulated with PELC induced more potent antigen-specific antibodies, hemagglutination inhibition, and virus neutralization than non-adjuvanted inactivated virus containing 5 mu g HA. In addition, T-cell proliferative responses, as well as interferon-gamma (IFN-gamma) and interleukin-4 (IL-4) secretion were significantly enhanced after immunization with PELC-adjuvanted inactivated virus. These results indicate that PELC can be used for effective single-dose immunization and could thus play an important role in influenza pandemic preparedness. (C) 2009 Elsevier Masson SAS. All rights reserved.
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