期刊
MICROBES AND INFECTION
卷 10, 期 8, 页码 885-891出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.micinf.2008.05.002
关键词
Plasmodium falciparum; glycosylphosphatidylinositol; anemia; red blood cell; membrane interaction
资金
- Vereinigung der Freunde des Tropeninstituts Hamburg
- ETH
- Swiss National Science Foundation [205321107651]
- Foundation Bay
- EMBO
Following exposure to synthetic Plasmodium falciparum glycosylphosphatidylinositol (P.f.-GPI), red blood cells (RBCs) reacted with antibodies in the serum of a patient with severe acute P. falciparum malaria. Carbohydrate microarray analysis of the patient's serum confirmed the presence of both, IgM and IgG antibodies against P.f.-GPI. The antibodies failed to bind to RBCs when P.f.-GPI lacking the lipid portion was applied. Addition of the detergent Triton X-100 during preincubation with P.f.-GPI resulted in increased recognition. Recognition of P.f.-GPI was dependent on the concentrations of synthetic P.f.-GPI, the serum and the numbers of RBCs. IgM antibodies dominated P.f.-GPI-sensitized RBCs recognition. Recognition by IgM antibodies proved highest during the 1st week of acute malaria and decreased during the following 2 weeks as assessed by flow cytometry and carbohydrate microarray analysis. These results strongly support the notion that released P.f.-GPI can insert into non-parasitized RBC membranes and results in recognition by circulating anti-GPI antibodies and possibly subsequent elimination. This process may contribute to malaria-associated anemia. (C) 2008 Elsevier Masson SAS. All rights reserved.
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