期刊
MICROBES AND INFECTION
卷 10, 期 4, 页码 367-373出版社
ELSEVIER
DOI: 10.1016/j.micinf.2007.12.009
关键词
avian infectious bronchitis virus; coronavirus; sialic acid; tracheal organ culture
Avian Infectious bronchitis virus (IBV) is a coronavirus that infects chickens via the respiratory epithelium as primary target cells. The binding of coronaviruses to the cell surface is mediated by the viral surface protein S. Recently we demonstrated that alpha 2,3-linked sialic acid serves as a receptor determinant for IBVon Vero cells and primary chicken embryo kidney cells. Here we analyze the importance of the sialic acid binding activity for the infection of tracheal organ cultures (TOCs) by different IBV strains. Our results show that alpha 2,3-linked sialic acid also serves as a receptor determinant on chicken TOCs. Infection of TOCs by IBV results in ciliostasis. Desialylation induced by neuraminidase treatment of tracheal organ cultures prior to infection by IBV delayed the ciliostatic effect or resulted in partial loss of ciliary activity. This effect was observed with both respiratory and nephropathogenic strains. Inhibition of ciliostasis was also observed when TOCs were pretreated with an alpha 2,3-specific neuraminidase. Analysis of the tracheal epithelium for reactivity with lectins revealed that the susceptible cells in the epithelium abundantly express alpha 2,3-linked sialic acid. These results indicate that alpha 2,3-linked sialic acid plays an important role for infection of the respiratory epithelium by IBV. (C) 2007 Elsevier Masson SAS. All rights reserved.
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