期刊
MICROBES AND ENVIRONMENTS
卷 29, 期 4, 页码 434-437出版社
JAPANESE SOC MICROBIAL ECOLOGY, DEPT BIORESOURCE SCIENCE
DOI: 10.1264/jsme2.ME14122
关键词
Burkholderia; Illumina sequencing; sugarcane field
资金
- KAKENHI [25292208]
- Program for Promotion of Basic and Applied Researches for Innovations in Bio-oriented Industry
- Grants-in-Aid for Scientific Research [26117732, 25292208, 14J09473] Funding Source: KAKEN
The diversity and abundance of Burkholderia species in sugarcane field soils were investigated by a 16S rRNA gene-based approach using genus-specific primers. A total of 365,721 sequences generated by the Illumina MiSeq platform were assigned to the genus Burkholderia. Nearly 58% of these sequences were placed in a previously defined cluster, including stinkbug symbionts. Quantitative PCR analysis revealed a consistent number of 16S rRNA gene copies for Burkholderia species (10(7) g(-1) soil) across the sampled fields. C/N, pH, and nitrate concentrations were important factors shaping the Burkholderia community structure; however, their impacts were not significant considering the overall genus size.
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