期刊
METHODS
卷 61, 期 2, 页码 110-116出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.ymeth.2013.02.010
关键词
Pyroptosis; Apoptosis; Cell death
资金
- Fundacao de Amparo A Pesquisa do Estado de Sao Paulo (FAPESP-Brazil)
- Brazilian Research Council (CNPq-Brazil)
- CAPES
Pyroptosis is a molecularly controlled form of cell death that exhibits some features of apoptosis as well of necrosis. Pyroptosis is induced by inflammasome-activated caspase-1 or caspase-11 (caspase-4 in humans), as a result of distinct pathogenic or damage stimuli. Although pyroptosis displays some morphological and biochemical features of apoptosis, it has an inflammatory outcome due to the loss of plasma membrane integrity and the consequent release of intracellular contents, reminiscent to necrosis. Here, we use cytosolic delivery of purified flagellin as an experimental tool to trigger pyroptosis and describe potential methods to study this form of cell death. Finally, we discuss the advantages and limitations of these methods. (C) 2013 Elsevier Inc. All rights reserved.
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