期刊
METHODS
卷 64, 期 1, 页码 73-78出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.ymeth.2013.05.026
关键词
Synchronized translation; Translation elongation; mRNA structure; G-quadruplex; Kinetic analysis
资金
- MEXT, Japan
- Nagase Science and Technology Foundation
- Hirao Taro Foundation of the Konan University Association for Academic Research
mRNA forms various secondary and tertiary structures that affect gene expression. Although structures formed in the untranslated regions (UTRs) of mRNAs that inhibit translation have been characterized, stable mRNA structures in open reading frames (ORFs) may also cause translational halt or slow translation elongation. We previously established a method, termed a synchronized translation assay, that enables time course analysis of single turnover translation elongation. In this method, translation initiation, which is a rate determining step of the translation procedure, can be ignored because all ribosomes are synchronized on a specific position of mRNA before translation elongation is restarted from this position. In this paper, we used the synchronized translation assay to evaluate the effects of a G-quadruplex structure located at various positions within the mRNA ORF on translational halt. (C) 2013 Elsevier Inc. All rights reserved.
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