期刊
METHODS
卷 53, 期 1, 页码 27-33出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.ymeth.2010.04.002
关键词
Membrane nanotubes; Laser scanning confocal microscopy; Live cell imaging; Gag-GFP; Filopodia; Cytoplasmic bridges
资金
- Wellcome Trust
- Medical Research Council [G0500563, G1001044] Funding Source: researchfish
- MRC [G0500563, G1001044] Funding Source: UKRI
A wide variety of cell types, including immune cells, have been observed to frequently interact via transient, long-distance membrane connections [1-17] However, considerable heterogeneity in their structure, mode of formation and functional properties has emerged, suggesting the existence of distinct subclasses [18-21]. Open-ended tunneling nanotubes allow for the trafficking of cytoplasmic material, e.g. endocytic vesicles, or the transmission of calcium signals [1,8]. Closed-ended membrane nanotubes do not seamlessly connect the cytoplasm between two interacting cells and a junction exists within the nanotube or where the nanotube meets a cell body [4,5,7]. Recent live cell imaging suggested that membrane nanotubes between T cells could present a novel route for HIV-1 transmission [7,22]. Here, we describe detailed protocols for observing membrane nanotubes and HIV-1 trafficking by live cell fluorescence microscopy. (C) 2010 Elsevier Inc. All rights reserved.
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