Article
Medical Laboratory Technology
Lily Olayinka, Izmarie Poventud-Fuentes, Emily Garnett, Sridevi Devaraj
Summary: Two pediatric cases had falsely elevated 25-hydroxyvitamin D levels, leading to unnecessary further testing. The addition of a centrifugation step resolved the variation in the measurement of 25-hydroxyvitamin D levels. Confirmation with instruments from a different vendor and LC-MS/MS was performed.
JOURNAL OF CLINICAL LABORATORY ANALYSIS
(2023)
Article
Endocrinology & Metabolism
Lauren A. Burt, Martin Kaufmann, Marianne S. Rose, Glenville Jones, Emma O. Billington, Steven K. Boyd, David A. Hanley
Summary: This randomized controlled trial investigated the effect of high-dose vitamin D-3 on total bone mineral density (TtBMD) in the radius and tibia. The study found that increasing vitamin D dose was associated with increased plasma levels of 25-(OH)D-3, 24,25-(OH)(2)D-3, and 1,24,25-(OH)(3)D-3, but not 1,25-(OH)(2)D-3. These vitamin D metabolites were associated with a decline in TtBMD. The findings suggest that vitamin D metabolites other than 1,25-(OH)(2)D-3 may play a role in bone loss.
JOURNAL OF BONE AND MINERAL RESEARCH
(2023)
Article
Medical Laboratory Technology
Tomas Matejek, Bara Zapletalova, Martin Stepan, Jana Malakova, Vladimir Palicka
Summary: The main objective of this study was to determine the levels of C3-epi-25(OH)D in very low birth weight infants. The study found a significant correlation between 25(OH)D and C3-epi-25(OH)D levels, and showed that C3-epi-25(OH)D accounts for more than 20% of total 25(OH)D at the end of the first month of life.
CLINICAL CHEMISTRY AND LABORATORY MEDICINE
(2023)
Article
Biochemistry & Molecular Biology
Artem Zhukov, Alexandra Povaliaeva, Zaur Abilov, Elena Kovaleva, Liliya Usoltseva, Anna Eremkina, Vitaliy Ioutsi, Larisa Dzeranova, Ekaterina Pigarova, Liudmila Rozhinskaya, Natalia Mokrysheva
Summary: This study evaluated the extended spectrum of vitamin D metabolites in patients with HypoPT compared to healthy individuals. The results showed that patients with HypoPT achieved an adequate level of 25(OH)D-3 with standard doses of cholecalciferol, but they had supraphysiological levels of 1,25(OH)(2)D-3 and increased production of inactive metabolite. The abnormality of vitamin D metabolism does not seem to interfere with the achievement of hypoparathyroidism compensation.
Article
Medical Laboratory Technology
Sieglinde Zelzer, Caroline Le Goff, Stephanie Peeters, Chiara Calaprice, Andreas Meinitzer, Dietmar Enko, Walter Goessler, Markus Herrmann, Etienne Cavalier
Summary: This study assessed the agreement of 24,25-dihydroxyvitamin D3 (24,25(OH)(2)D-3) measurements by two in-house LC-MS/MS methods. The results showed high precision measurements of 24,25(OH)(2)D-3 with both methods, despite minor differences between them.
CLINICAL CHEMISTRY AND LABORATORY MEDICINE
(2022)
Article
Biochemical Research Methods
Christopher T. Sempos, Emma L. Williams, Graham D. Carter, Julia Jones, Johanna E. Camara, Carolyn Q. Burdette, Grace Hahm, Federica Nalin, David L. Duewer, Adam J. Kuszak, Joyce Merkel, Andrew N. Hoofnagle, Pierre Lukas, Etienne Cavalier, Ramon A. Durazo-Arvizu, Peter M. Crump, Christian Popp, Christian Beckert, Jan Schultess, Glen Van Slooten, Carole Tourneur, Camille Pease, Ravi Kaul, Alfredo Villarreal, Fiona Ivison, Ralf Fischer, Jody M. W. van den Ouweland, Chung S. Ho, Emmett W. K. Law, Jean-Nicolas Simard, Renaud Gonthier, Brett Holmquist, Marcelo Cidade Batista, Sarah Meadows, Lorna Cox, Eugene Jansen, Dilshad Ahmed Khan, Kimberly Robyak, Michael H. Creer, Mark Kilbane, Patrick J. Twomey, James Freeman, Neil Parker, Jinyun Yuan, Robert Fitzgerald, Sohail Mushtaq, Michael W. Clarke, Norma Breen, Christine Simpson, Stephen A. Wise
Summary: This study compared the results of DEQAS human serum samples shipped under frozen and ambient conditions, finding significant differences in certain 25(OH)D assays depending on the handling/transport conditions. The LC-MS/MS methods showed stability during shipment at ambient conditions, whereas some ligand binding assays exhibited discrepancies.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Analytical
R. Rola, K. Kowalski, T. Bienkowski, J. Witwicki, S. Studzinska
Summary: The study demonstrated the utility of DBS samples in assessing newborns' vitamin D levels, comparing them to maternal and cord blood-derived serum tests. Results showed that DBS could be used interchangeably with cord blood-derived serum to determine vitamin D status in neonates, simplifying the screening process.
MICROCHEMICAL JOURNAL
(2021)
Article
Biochemistry & Molecular Biology
Dae-Hyun Ko, Sun-Hee Jun, Youngwon Nam, Sang H. Song, Minje Han, Yeo-Min Yun, Kyunghoon Lee, Junghan Song
Summary: A multiplex LC-MS/MS method was developed to determine bioavailable vitamin D and VMR, showing differences in bioavailable vitamin D levels among different ethnicities. The new method was robust and reliable, suitable for routine clinical samples.
JOURNAL OF STEROID BIOCHEMISTRY AND MOLECULAR BIOLOGY
(2021)
Review
Nutrition & Dietetics
Pedro Araujo, Cioly Mendez-Davila
Summary: This article discusses the analytical challenges associated with quantifying vitamin D intake, including studying assay variability before categorizing a method as the golden standard, ensuring sample comparability for meaningful correlations, revising intake guidance based on statistical power analysis, and implementing strategies to prevent preanalytical factors. Addressing these challenges will enable effective vitamin D management in the sports sector.
FRONTIERS IN NUTRITION
(2021)
Article
Endocrinology & Metabolism
Cora M. Best, Leila R. Zelnick, Kenneth E. Thummel, Simon Hsu, Christine Limonte, Ravi Thadhani, Howard D. Sesso, JoAnn E. Manson, Julie E. Buring, Samia Mora, I-Min Lee, Nancy R. Cook, Georgina Friedenberg, Heike Luttmann-Gibson, Ian H. de Boer, Andrew N. Hoofnagle
Summary: Vitamin D-3 supplementation increases total serum vitamin D and 25(OH) D concentrations with variation according to baseline 25(OH)D, which suggests that 25-hydroxylation of vitamin D-3 is more efficient when serum 25(OH)D concentration is low.
JOURNAL OF CLINICAL ENDOCRINOLOGY & METABOLISM
(2022)
Article
Biochemistry & Molecular Biology
Nazli Ecem Dal-Bekar, Gul Huray Islekel, Aydan Koken-Avsar, Handan Yarkan-Tugsal, Gamze Tuna, Berrin Zengin, Ahmet Merih Birlik
Summary: Scleroderma is a rare autoimmune disease characterized by progressive fibrosis of the skin and internal organs. Oxidative damage and vitamin D deficiency play important roles in the development of scleroderma. This study comprehensively investigated oxidative DNA damage in scleroderma and evaluated the effects of vitamin D supplementation.
JOURNAL OF STEROID BIOCHEMISTRY AND MOLECULAR BIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Akiko Nagata, Kazuto Iijima, Ryota Sakamoto, Yuka Mizumoto, Miho Iwaki, Masaki Takiwaki, Yoshikuni Kikutani, Seketsu Fukuzawa, Minami Odagi, Masayuki Tera, Kazuo Nagasawa
Summary: This study describes a versatile method for synthesizing deuterium-labeled D-3 metabolites and successfully applies it to measure the concentration of these compounds in pooled human serum. This includes the first quantification of 1,25(OH)(2)D-3-23,26-lactone in human serum.
Article
Medical Laboratory Technology
Ashley S. P. Boggs, Lisa E. Kilpatrick, Carolyn Q. Burdette, Denise S. Tevis, Zachary A. Fultz, Michael A. Nelson, Jeffery M. Jarrett, Jennifer Kemp, Ravinder J. Singh, Stefan K. G. Grebe, Stephen A. Wise, Brittany L. Kassim, Stephen E. Long
Summary: This study developed Standard Reference Material 1949 for measuring hormone and nutrient concentrations during pregnancy. Significant differences were found in serum concentrations across different trimesters, especially in TSH and Tg measurements. The results indicate the material functions well by providing a relevant matrix-matched reference material.
CLINICAL CHEMISTRY AND LABORATORY MEDICINE
(2021)
Article
Nutrition & Dietetics
Maryam Razaghi, Nathalie Gharibeh, Catherine A. Vanstone, Olusola F. Sotunde, Ali Khamessan, Shu Qin Wei, Dayre McNally, Frank Rauch, Glenville Jones, Sarah Kimmins, Hope A. Weiler
Summary: Supplementing with vitamin D during infancy improves vitamin D levels, and a daily dose of 1000 IU increases lean mass slightly.
AMERICAN JOURNAL OF CLINICAL NUTRITION
(2022)
Review
Chemistry, Analytical
A. Fernez Asensio, E. Alvarez-Gonzalez, A. Rodriguez, L. M. Sierra, E. Blanco-Gonzalez
Summary: Dysregulation of TCA cycle enzymes leads to abnormal accumulation of oncometabolites, which contribute to cancer formation and progression. GC-MS and LC-MS are powerful analytical tools for accurate identification and determination of these metabolites.
ANALYTICA CHIMICA ACTA
(2021)
Article
Biochemical Research Methods
Stephen A. Wise, Johanna E. Camara, Carolyn Q. Burdette, Grace Hahm, Federica Nalin, Adam J. Kuszak, Joyce Merkel, Ramon A. Durazo-Arvizu, Emma L. Williams, Andrew N. Hoofnagle, Fiona Ivison, Ralf Fischer, Jody M. W. van den Ouweland, Chung S. Ho, Emmett W. K. Law, Jean-Nicolas Simard, Renaud Gonthier, Brett Holmquist, Sarah Meadows, Lorna Cox, Kimberly Robyak, Michael H. Creer, Robert Fitzgerald, Michael W. Clarke, Norma Breen, Pierre Lukas, Etienne Cavalier, Christopher T. Sempos
Summary: The Vitamin D Standardization Program (VDSP) conducted an interlaboratory comparison study to evaluate the performance of liquid chromatography - tandem mass spectrometry (LC-MS/MS) assays for determining serum total 25-hydroxyvitamin D. Results showed that only 53% of the assays met the VDSP criterion, with four assays having significant performance issues due to the lack of separation between 3-epi-25(OH)D-3 and 25(OH)D-3.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2022)
Article
Biochemical Research Methods
Christopher T. Sempos, Emma L. Williams, Graham D. Carter, Julia Jones, Johanna E. Camara, Carolyn Q. Burdette, Grace Hahm, Federica Nalin, David L. Duewer, Adam J. Kuszak, Joyce Merkel, Andrew N. Hoofnagle, Pierre Lukas, Etienne Cavalier, Ramon A. Durazo-Arvizu, Peter M. Crump, Christian Popp, Christian Beckert, Jan Schultess, Glen Van Slooten, Carole Tourneur, Camille Pease, Ravi Kaul, Alfredo Villarreal, Fiona Ivison, Ralf Fischer, Jody M. W. van den Ouweland, Chung S. Ho, Emmett W. K. Law, Jean-Nicolas Simard, Renaud Gonthier, Brett Holmquist, Marcelo Cidade Batista, Sarah Meadows, Lorna Cox, Eugene Jansen, Dilshad Ahmed Khan, Kimberly Robyak, Michael H. Creer, Mark Kilbane, Patrick J. Twomey, James Freeman, Neil Parker, Jinyun Yuan, Robert Fitzgerald, Sohail Mushtaq, Michael W. Clarke, Norma Breen, Christine Simpson, Stephen A. Wise
Summary: This study compared the results of DEQAS human serum samples shipped under frozen and ambient conditions, finding significant differences in certain 25(OH)D assays depending on the handling/transport conditions. The LC-MS/MS methods showed stability during shipment at ambient conditions, whereas some ligand binding assays exhibited discrepancies.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2022)
Article
Biochemistry & Molecular Biology
Monica L. Brown Lobbins, Andrzej T. Slominski, Karen A. Hasty, Sicheng Zhang, Duane D. Miller, Wei Li, Tae-Kang Kim, Zorica Janjetovic, Robert C. Tuckey, Imara-Safi O. Scott, Linda K. Myers, Arnold E. Postlethwaite
Summary: This study demonstrates that 17,20S(OH)(2)pD can suppress type I collagen synthesis and decrease the expression of fibrosis-related mediators, offering a potential noncalcemic secosteroidal therapeutic approach for treating systemic sclerosis and fibrosis.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Multidisciplinary Sciences
Tanner J. Varrelman, Christopher H. Remien, Andrew J. Basinski, Shelley Gorman, Alec Redwood, Scott L. Nuismer
Summary: This study evaluates the effectiveness of using betaherpesviruses as transmissible vaccine vectors. The mathematical model developed and parameterized using data from mouse populations infected with murine cytomegalovirus (MCMV) shows rapid and effective control of pathogens with the introduction of the transmissible vaccine. However, the effectiveness may vary across reservoir populations and specific vector strains used for the vaccine.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Physiology
Erin L. Fee, Tsukasa Takahashi, Yuki Takahashi, Sean Carter, Lucy Furfaro, Michael W. Clarke, Mark A. Milad, Haruo Usuda, John P. Newnham, Masatoshi Saito, Alan H. Jobe, Matthew W. Kemp
Summary: Antenatal steroids play an important role in promoting functional maturation of the fetal lung. Current dosing regimens may pose potential risks, but this study found that direct fetal administration of a low dose of betamethasone phosphate can achieve the same lung maturation without maternal steroid administration.
AMERICAN JOURNAL OF PHYSIOLOGY-LUNG CELLULAR AND MOLECULAR PHYSIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Pawel Brzeminski, Adrian Fabisiak, Radomir M. Slominski, Tae-Kang Kim, Zorica Janjetovic, Ewa Podgorska, Yuwei Song, Mohammad Saleem, Sivani B. Reddy, Shariq Qayyum, Yuhua Song, Robert C. Tuckey, Venkatram Atigadda, Anton M. Jetten, Rafal R. Sicinski, Chander Raman, Andrzej T. Slominski
Summary: This study established new routes for the chemical synthesis of vitamin D-3 and its hydroxy metabolites. The compounds showed similar properties in inhibiting cell proliferation and inducing cell differentiation, as well as effects on T-cell immune activity. They also interacted with nuclear receptors in a similar manner. The findings have important implications for studying the role of these compounds in the immune system and inflammatory diseases.
BIOORGANIC CHEMISTRY
(2022)
Article
Biochemistry & Molecular Biology
Yuwei Song, Radomir M. Slominski, Shariq Qayyum, Tae-Kang Kim, Zorica Janjetovic, Chander Raman, Robert C. Tuckey, Yuhua Song, Andrzej T. Slominski
Summary: This study used molecular simulation to investigate the interaction between vitamin D3 hydroxyderivatives and AhR. It was found that these derivatives can stimulate downstream target genes of AhR. Molecular dynamics simulations showed favorable binding of the derivatives to AhR and revealed potential mechanisms underlying their binding affinities.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2022)
Article
Biochemistry & Molecular Biology
Andrzej T. Slominski, Tae-Kang Kim, Radomir M. Slominski, Yuwei Song, Zorica Janjetovic, Ewa Podgorska, Sivani B. Reddy, Yuhua Song, Chander Raman, Edith K. Y. Tang, Adrian Fabisiak, Pawel Brzeminski, Rafal R. Sicinski, Venkatram Atigadda, Anton M. Jetten, Michael F. Holick, Robert C. Tuckey
Summary: CYP11A1 and CYP27A1 hydroxylate tachysterol(3), a photoproduct of previtamin D-3, producing 20S-hydroxytachysterol(3) and 25(OH)T-3, which are biologically active and interact with VDR, AhR, LXRs, and PPAR gamma. These metabolites inhibit cell proliferation and stimulate differentiation and anti-oxidative gene expression in keratinocytes and fibroblasts.
Article
Oncology
Andrzej T. Slominski, Anna A. Brozyna, Tae-Kang Kim, Mahmoud M. Elsayed, Zorica Janjetovic, Shariq Qayyum, Radomir M. Slominski, Allen S. W. Oak, Changzhao Li, Ewa Podgorska, Wei Li, Anton M. Jetten, Robert C. Tuckey, Edith K. Y. Tang, Craig Elmets, Mohammad Athar
Summary: Hydroxyderivatives of vitamin D3 have anti-cancer activities by acting on vitamin D receptor and retinoid-related orphan receptors, inhibiting cell proliferation and tumor formation.
INTERNATIONAL JOURNAL OF ONCOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Robert C. Tuckey, Chloe Y. S. Cheng, Lei Li, Yuhan Jiang
Summary: Our study found that 25-hydroxyvitamin D3 (25(OH)D3) can exist in the human circulation in the sulfated form, and can be metabolized by vitamin D-metabolizing cytochromes P450 (CYPs). Specifically, CYP27A1 can convert vitamin D3 sulfate to 25(OH)D3 3-sulfate, while CYP11A1 can convert vitamin D3 sulfate to 20(OH)D3 3-sulfate, but at a lower rate.
JOURNAL OF STEROID BIOCHEMISTRY AND MOLECULAR BIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Warusavithana Gunawardena Manori De Silva, Bianca Yuko McCarthy, Jeremy Han, Chen Yang, Andrew J. A. Holland, Harvey Stern, Katie Marie Dixon, Edith Kai Yan Tang, Robert Charles Tuckey, Mark Stephen Rybchyn, Rebecca Sara Mason
Summary: In this study, it was found that the over-irradiation metabolite 24(OH)L-3 provided photoprotection against UV-induced DNA damage similar to that of 1,25(OH)(2)D-3. The mechanisms involved increased energy availability and DNA repair, as well as decreased reactive oxygen species.
Article
Physiology
Erin L. Fee, Tsukasa Takahashi, Yuki Takahashi, Sean W. D. Carter, Michael W. Clarke, Mark A. Milad, Haruo Usuda, Hideyuki Ikeda, Yusaku Kumagai, Yuya Saito, Demelza J. Ireland, John P. Newnham, Masatoshi Saito, Alan H. Jobe, Matthew W. Kemp
Summary: This study aimed to determine the lowest fetal plasma betamethasone concentration sufficient to achieve functional preterm lung maturation and found that treatment efficacy was progressively lost at plasma betamethasone concentrations lower than 2 ng/mL.
AMERICAN JOURNAL OF PHYSIOLOGY-LUNG CELLULAR AND MOLECULAR PHYSIOLOGY
(2023)
Editorial Material
Dermatology
Andrzej T. Slominski, Robert C. Tuckey, Anton M. Jetten, Michael F. Holick
JOURNAL OF INVESTIGATIVE DERMATOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Lei Li, Robert C. Tuckey
Summary: Vitamin D exists in two forms, D3 produced in the skin and D2 obtained from the diet. The enzyme CYP24A1 plays a crucial role in oxidizing and inactivating both forms of vitamin D, regulating their serum levels. This study aimed to clarify the pathways of oxidation for 25(OH)D2 and 1,25(OH)(2)D2 and compare their metabolism with their vitamin D3 counterparts.
JOURNAL OF STEROID BIOCHEMISTRY AND MOLECULAR BIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Dongxian Wu, Gareth Nealon, Yuchen Liu, Tae-Kang Kim, Andrzej T. Slominski, Robert C. Tuckey
Summary: Lumisterol2 (L2) is a photoproduct of UVB action on ergosterol, which is present in edible mushrooms and human skin. CYP27A1 efficiently metabolizes L2 to produce three major hydroxy-L2 products, while CYP11A1 has minimal effect on L2 metabolism. The major CYP27A1-derived hydroxy-L2 products have anti-proliferative activity on skin-cancer cell lines.
JOURNAL OF STEROID BIOCHEMISTRY AND MOLECULAR BIOLOGY
(2023)
