Review
Biotechnology & Applied Microbiology
Shengzhu Yu, Bo Zheng, Zhenya Chen, Yi-Xin Huo
Summary: Branched chain amino acids have been synthesized through metabolic engineering in Corynebacterium glutamicum. Further research should focus on the feedback inhibition and transcription attenuation mechanisms of key enzymes, as well as the development of enzymes with substrate specificity for the production of individual BCAAs. The strategies used in constructing BCAAs-producing strains provide valuable guidance for the biosynthesis of other high value-added compounds.
MICROBIAL CELL FACTORIES
(2021)
Article
Microbiology
Hedan Li, Daqing Xu, Dezhi Zhang, Xin Tan, Danyang Huang, Wenjian Ma, Guihong Zhao, Ying Li, Ziwei Liu, Yang Wang, Xiaoqing Hu, Xiaoyuan Wang
Summary: In this study, the researchers investigated the effect of biosynthesis of trehalose dicorynomycolates on L-isoleucine production in C. glutamicum by deleting several genes. They found that the production of L-isoleucine was significantly improved in the triple deletion mutant ?treSYA. Further deletion of mytA or mytB in ?treSYA could further enhance the production, but deletion of both mytA and mytB significantly decreased the production. Ultimately, they constructed the final L-isoleucine producing strain WL001, which showed a 36.1% increase in L-isoleucine production compared to the control strain WM001 after 72 hours of shake flask cultivation.
MICROBIOLOGICAL RESEARCH
(2023)
Article
Engineering, Chemical
Zaiwei Man, Jin Li, Huihui Cui, Zhiqiang Cai, Jing Guo
Summary: This study optimized the L-citrulline biosynthesis operon in C. glutamicum and successfully constructed an efficient L-citrulline-producing strain. The L-citrulline production was further improved by plasmid-based overexpression of the optimized operon.
Review
Biotechnology & Applied Microbiology
Min Duan, Shuo Chen, Xinli Liu, Jianhang Liu, Deqiang Zhu
Summary: This article provides a comprehensive review of the function and application of l-threonine, the pathway of C. glutamicum to synthesize l-threonine, and the use of metabolic engineering technology to improve its production.
FERMENTATION-BASEL
(2023)
Article
Multidisciplinary Sciences
Yan-Feng Guo, Yu Lu, Lei Song, Jinghua Liu, Yi-Lei Wang, Guo-Qing Meng
Summary: The amino acid mutations of Gly92Asp, Ile162Val, Arg494His and Gly526Asp were found in the a-IPMS variant from strain ARTP-L04, which exhibited higher resistance to feedback inhibition by L-leucine. The activity of the a-IPMS variant was still retained at over 50% in the presence of 20 mM L-leucine. There was no significant difference in specific activities between the a-IPMS variant and wild-type a-IPMS.
Article
Biochemical Research Methods
Alex Huang, Cathy Coutu, Myrtle Harrington, Kevin Rozwadowski, Dwayne D. Hegedus
Summary: Camelina sativa is being recognized as an alternative oilseed crop with the potential for increased lysine content through genetic modification. Mutant forms of DHDPS in C. sativa show reduced sensitivity to lysine, allowing for the production of higher quality animal feed.
TRANSGENIC RESEARCH
(2022)
Article
Biochemistry & Molecular Biology
Yanchao Zhang, Yadi Liu, Shuyan Zhang, Wenjian Ma, Jianli Wang, Lianghong Yin, Xiaoyuan Wang
Summary: In this study, l-isoleucine production in Corynebacterium glutamicum WM001 was significantly improved through gene deletion, promoter replacement, and gene overexpression. The best yield of 32.1 g/L l-isoleucine was obtained from strain WM005/pYCW-1-ilvBN2-ppnK1 after 72-hour fed-batch fermentation, representing a 34.3% increase compared to the original strain WM001.
BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY
(2021)
Article
Biotechnology & Applied Microbiology
Eldin Kurpejovic, Volker F. Wendisch, Berna Sariyar Akbulut
Summary: The increase in elderly patients with Parkinson's disease is driving market growth for l-DOPA, with biotechnological approaches in Corynebacterium glutamicum cells showing potential for economically feasible production.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2021)
Article
Agricultural Engineering
Qi Sheng, Xiaoyu Wu, Yan Jiang, Zhimin Li, Fei Wang, Bin Zhang
Summary: This study demonstrated the biobased production of L-ornithine from mannitol using engineered Corynebacterium glutamicum, providing a reference for the production of additional chemicals from mannitol.
BIORESOURCE TECHNOLOGY
(2021)
Article
Engineering, Chemical
Zaiwei Man, Huihui Cui, Jin Li, Zhiqiang Cai, Jing Guo
Summary: In this study, the overexpression of homologous TreS significantly improved trehalose production by simplifying the process. Using permeabilized cells as biocatalysts, trehalose synthesis could be achieved efficiently in a short period of time.
Article
Food Science & Technology
Bingnan Liu, Xinyu Sun, Yue Liu, Mengmeng Yang, Liang Wang, Ying Li, Jihui Wang
Summary: This study successfully increased the supply of reducing power NADPH and improved the production of L-methionine through genetic modification and heterologous expression. The engineered strain YM6 with high NADPH supply and high L-methionine production was constructed, and metabolic flux analysis confirmed the increase in its synthesis.
Article
Endocrinology & Metabolism
Chunjun Zhan, Namil Lee, Guangxu Lan, Qingyun Dan, Aidan Cowan, Zilong Wang, Edward E. K. Baidoo, Ramu Kakumanu, Bridget Luckie, Rita C. Kuo, Joshua McCauley, Yuzhong Liu, Luis Valencia, Robert W. Haushalter, Jay D. Keasling
Summary: Corynebacterium glutamicum is a promising host for producing valuable polyketides, but propionate addition inhibits its growth. This study identifies the accumulation of propionyl-CoA and methylmalonyl-CoA as the cause of growth inhibition and demonstrates that it can be relieved by introducing methylmalonyl-CoA-dependent polyketide synthases. Laboratory evolution is used to increase polyketide production by targeting mutations in germicidin synthase and citrate synthase. These findings highlight the importance and potential of C. glutamicum in polyketide production.
Article
Agriculture, Multidisciplinary
Jaehyun Park, Han Min Woo
Summary: Corynebacterium glutamicum has been engineered for co-production of L-lysine and heterologous squalene, and metabolic fluxes were controlled by regulating the concentration of pyruvate, an intermediate in the central metabolism. In situ extraction with oil overlay showed high efficiency in co-production.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2022)
Article
Microbiology
Jie Liu, Jian-Zhong Xu, Zhi-Ming Rao, Wei-Guo Zhang
Summary: This article briefly introduces the wide application of L-lysine in food, pharmaceuticals, and feed additives, as well as its production and regulation mechanism in Corynebacterium glutamicum. It also details the strategies for constructing high-producing strains, scaling up culture, and separating and purifying the product, as well as new breeding and fermentation methods.
MICROBIOLOGICAL RESEARCH
(2022)
Review
Biotechnology & Applied Microbiology
Yan Jiang, Qi Sheng, Xiao-Yu Wu, Bang-Ce Ye, Bin Zhang
Summary: This review summarizes recent advances in molecular biology strategies for optimizing l-arginine-producing strains, including various metabolic engineering methods to reduce production costs, providing valuable insights into the current state of l-arginine production.
CRITICAL REVIEWS IN BIOTECHNOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Nicolas Huber, Edgar Alberto Alcala-Orozco, Thomas Rexer, Udo Reichl, Steffen Klamt
Summary: Cell-free production systems are commonly used for synthesizing industrial chemicals and biopharmaceuticals. This study presents a model-based optimization framework for cell-free enzyme cascades, taking into account parameter uncertainties. The approach was exemplified using the synthesis of GDP-fucose, resulting in significant improvements in the process.
METABOLIC ENGINEERING
(2024)
