Article
Biochemistry & Molecular Biology
Tong Wei, Yuetong Guo, Chenglin Huang, Mengwei Sun, Bin Zhou, Jing Gao, Weili Shen
Summary: This study found that angiotensin II (Ang II) promotes the differentiation of cardiac fibroblasts (CFs) into myofibroblasts and alters their metabolic patterns. GCN5L1 protein interacts with and acetylates mitochondrial pyruvate carrier 2 (MPC2), hindering mitochondrial pyruvate uptake and respiration. Additionally, blocking monocarboxylate transporter 1 (MCT1) disrupts the lactate shuttle from CFs to cardiomyocytes, mitigating cardiac remodeling.
CELL AND BIOSCIENCE
(2023)
Article
Biochemistry & Molecular Biology
Michele Mannelli, Tania Gamberi, Francesca Magherini, Tania Fiaschi
Summary: This study demonstrates that cachexia induction in myotubes is associated with a metabolic shift towards fermentation, and inhibiting lactate formation can prevent cachexia. The findings suggest lactate dehydrogenase as a possible new tool for counteracting the onset of this pathology.
Review
Biochemistry & Molecular Biology
Edward Caddye, Julien Pineau, Joshua Reyniers, Itamar Ronen, Alessandro Colasanti
Summary: Lactate, as a metabolic intermediate, shows potential as a biomarker for neurometabolic dysfunction in psychiatric disorders. Lactate response reflects metabolic and mitochondrial health, its concentration is regulated by the sleep-wake cycle, and it integrates and transmits information about arousal behaviors and cellular metabolism.
Article
Computer Science, Interdisciplinary Applications
Wil Jones, Dimitrios I. Gerogiorgis
Summary: This paper presents dynamic simulations and optimizations for Fed-batch and perfusion reactors to increase the production of monoclonal antibodies (mAbs). Detailed technoeconomic analysis demonstrates the advantages of Fed-batch reactors and the cost-related promise of perfusion bioreactor technologies.
COMPUTERS & CHEMICAL ENGINEERING
(2022)
Article
Chemistry, Multidisciplinary
Junlong Zhao, Zhimin Tian, Shoujie Zhao, Dayun Feng, Zhixiong Guo, Liangzhi Wen, Yejing Zhu, Fenghua Xu, Jun Zhu, Shouzheng Ma, Jie Hu, Tao Jiang, Yongquan Qu, Dongfeng Chen, Lei Liu
Summary: This study illustrates the instructive relationships between intratumoral lactate regulation, metabolic reprogramming, and immune activation for catalytic immunotherapy of tumors. The catalysts efficiently oxidize intratumoral lactate, induce tumor cell apoptosis, reprogram metabolism, and activate antitumor immunity.
Review
Cell Biology
Xuelian Liu, Yu Zhang, Wei Li, Xin Zhou
Summary: Lactate functions as a carbon source, signaling molecule, and immune regulator. Lactylation, regulated by lactate, has been confirmed as a novel contributor to epigenetic landscape. Protein lactylation plays a pivotal role in cell fate determination, embryonic development, inflammation, cancer, and neuropsychiatric disorders.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Lucia Longhitano, Sebastiano Giallongo, Laura Orlando, Giuseppe Broggi, Antonio Longo, Andrea Russo, Rosario Caltabiano, Cesarina Giallongo, Ignazio Barbagallo, Michelino Di Rosa, Rosario Giuffrida, Rosalba Parenti, Giovanni Li Volti, Nunzio Vicario, Daniele Tibullo
Summary: This study aimed to investigate the role of lactate in uveal melanoma (UM) cells. The results showed that lactate could inhibit cell proliferation and migration, and switch cell metabolism towards oxidative phosphorylation. It was also found that an increase in lactate transporters MCT4 and HCAR1 was associated with a spindle-shape histological type in UM. These findings suggest that lactate metabolism may serve as a prognostic marker of UM progression and a potential therapeutic target.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Immunology
Laura Barbieri, Pedro Velica, Paulo A. Gameiro, Pedro P. Cunha, Iosifina P. Foskolou, Eric Rullman, David Bargiela, Randall S. Johnson, Helene Rundqvist
Summary: CD8+ T cells can tolerate lactate anion at neutral pH and use it as an alternative energy source to glucose. The presence of lactate alters the transcriptome of CD8+ T cells and affects their differentiation and activity.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Agricultural Engineering
Nicolas Ramirez, Claudia Ubilla, Javiera Campos, Francisca Valencia, Carla Aburto, Carlos Vera, Andres Illanes, Cecilia Guerrero
Summary: The study evaluated the effects of operational variables on lactulose production by enzymatic transgalactosylation in fed-batch and repeated fed-batch. Feed flowrate and fructose to lactose molar ratio were the most significant variables affecting lactulose yield, productivity, and selectivity. The repeated fed-batch operation increased efficiency of biocatalyst use and accumulated productivity significantly compared to batch and fed-batch operations with the same biocatalyst.
BIORESOURCE TECHNOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Daniela Velazquez, Juan-Carlos Sigala, Luz Maria Martinez, Paul Gaytan, Guillermo Gosset, Alvaro R. Lara
Summary: This study selected a mutant strain that mimics Fed-batch behavior in batch cultures through cell engineering and high-throughput screening, and the amount of GFP produced by this strain is significantly higher than that of the W3110 strain in both batch and Fed-batch modes.
MICROBIAL CELL FACTORIES
(2022)
Article
Engineering, Environmental
Gisela Nadal-Rey, Dale D. McClure, John M. Kavanagh, Benny Cassells, Sjef Cornelissen, David F. Fletcher, Krist Gernaey
Summary: The study presents a novel methodology using dynamic compartment models to simulate fed-batch fermentation processes, allowing for rapid characterization and assessment of the impact of gradients on process performance in industrial settings. The models were validated numerically and can be readily applied to various types of bioreactors and microorganisms.
CHEMICAL ENGINEERING JOURNAL
(2021)
Article
Biotechnology & Applied Microbiology
Agustina Godino, Marilla Amaranto, Agustina Manassero, Fausto Comba, Martin Alonzo Perez, Lucio Simonella, Martin Pernigotti, Jose L. Barra
Summary: In this study, a recombinant His-tagged LOX enzyme was successfully produced in Escherichia coli and characterized for industrial applications. The rLOX showed comparable performance to commercial untagged LOXs in a colorimetric kit for human diagnostics and an amperometric biosensor for measuring lactic acid in food products. The production of rLOX was scaled up in a fed-batch bioreactor culture, resulting in a tenfold increase in yield compared to the Erlenmeyer scale. The His-tag facilitated efficient purification, leading to the recovery of a high-purity rLOX after one-step affinity purification.
JOURNAL OF BIOTECHNOLOGY
(2023)
Article
Biotechnology & Applied Microbiology
Sara Moreno-Paz, Joep Schmitz, Vitor A. P. Martins dos Santos, Maria Suarez-Diez
Summary: This study assessed the accuracy and predictive power of genome-scale, constraint-based models in predicting metabolic changes in response to operational conditions in a bioreactor and intracellular, active reactions. The findings showed that the enzyme-constrained version of the model outperformed the original model in all simulations. The combination of this model with dynamic FBA allowed for the prediction of yields and productivities of different strains and production processes. Additionally, constraining protein availability improved the accuracy of the metabolic state description under dynamic conditions. These findings have important implications for the design of industrially relevant cell-based processes.
MICROBIAL BIOTECHNOLOGY
(2022)
Review
Chemistry, Multidisciplinary
Huocheng Yang, Xuemei Yao, Yingqi Liu, Xinkun Shen, Menghuan Li, Zhong Luo
Summary: Ferroptosis, a regulated cell death driven by iron-dependent phospholipid peroxidation, has gained attention in nanomedicine since 2012. It has unique features compared to other cell death modes and shows promise in treating resistant cancers. Recent insights suggest its connection to the tumor immune microenvironment, indicating its potential in stimulating antitumor immunity. Understanding the relationship between tumor metabolism and ferroptosis is important for developing antitumor therapies.
Article
Microbiology
Gopinath Krishnamoorthy, Peggy Kaiser, Patricia Constant, Ulrike Abu Abed, Monika Schmid, Christian K. Frese, Volker Brinkmann, Mamadou Daffe, Stefan H. E. Kaufmann
Summary: Mycofactocin, a peptide-derived redox cofactor, is found in certain bacteria like Mycobacterium tuberculosis. Research indicates that mftD gene expression is upregulated in hypoxia-adapted M. tuberculosis, but its functionality may not be essential for L-lactate metabolism. The study highlights the potential significance of mycofactocin in bacterial growth and persistence within the host.
Article
Oncology
Gar Yee Koh, Anne Kane, Kyongbum Lee, Qiaobing Xu, Xian Wu, Jatin Roper, Joel B. Mason, Jimmy W. Crott
INTERNATIONAL JOURNAL OF CANCER
(2018)
Article
Cell Biology
Smitha Krishnan, Yufang Ding, Nima Saedi, Maria Choi, Gautham V. Sridharan, David H. Sherr, Martin L. Yarmush, Robert C. Alaniz, Arul Jayaraman, Kyongbum Lee
Article
Engineering, Chemical
Zhenyu Wang, Hana Sheikh, Kyongbum Lee, Christos Georgakis
Review
Pharmacology & Pharmacy
Yufang Ding, Karin Yanagi, Clint Cheng, Robert C. Alaniz, Kyongbum Lee, Arul Jayaraman
PHARMACOLOGICAL RESEARCH
(2019)
Article
Chemistry, Medicinal
Hyejin Park, Un-Ho Jin, Asuka A. Orr, Stephanie P. Echegaray, Laurie A. Davidson, Clinton D. Allred, Robert S. Chapkin, Arul Jayaraman, Kyongbum Lee, Phanourios Tamamis, Stephen Safe
CHEMICAL RESEARCH IN TOXICOLOGY
(2019)
Article
Microbiology
Ming Lei, Rani Menon, Sara Manteiga, Nicholas Alden, Carrie Hunt, Robert C. Alaniz, Kyongbum Lee, Arul Jayaraman
Article
Biochemistry & Molecular Biology
Neda Hassanpour, Nicholas Alden, Rani Menon, Arul Jayaraman, Kyongbum Lee, Soha Hassoun
Article
Biochemistry & Molecular Biology
Nicholas Alden, Ravali Raju, Kyle McElearney, James Lambropoulos, Rashmi Kshirsagar, Alan Gilbert, Kyongbum Lee
Article
Gastroenterology & Hepatology
Xi Qian, Karin Yanagi, Anne Kane, Nicholas Alden, Ming Lei, David R. Snydman, Richard J. Vickers, Kyongbum Lee, Cheleste M. Thorpe
AMERICAN JOURNAL OF PHYSIOLOGY-GASTROINTESTINAL AND LIVER PHYSIOLOGY
(2020)
Article
Cell Biology
Ying-Shiuan Chen, Jia Li, Sultan Neja, Sabeeta Kapoor, Jorge Enrique Tovar Perez, Chakrapani Tripathi, Rani Menon, Arul Jayaraman, Kyongbum Lee, Wan Mohaiza Dashwood, Shan Wang, Ke Zhang, Koichi Kobayashi, Praveen Rajendran, Roderick Dashwood
Summary: This study reveals the link between spinach-derived linoleate and butanoate metabolites and anticancer effects in a genetic model of colorectal cancer. Mechanistic studies demonstrate that these metabolites target HDAC activity and the IFN-gamma signaling axis. The clinical translation of these findings could have significant implications for delaying surgical interventions and drug therapies in at-risk patients.
Article
Biochemistry & Molecular Biology
Grace Yao, Kathryn Aron, Michael Borys, Zhengjian Li, Girish Pendse, Kyongbum Lee
Summary: The research identified potential high productivity indicators in CHO cell clones, such as citrate, which improved qP by up to 490% when used as a medium additive. This demonstrates the potential of metabolomics in discovering novel metabolite additives for higher volumetric productivity in biologics production processes.
Article
Biotechnology & Applied Microbiology
Ming Lei, Vikas D. Trivedi, Nikhil U. Nair, Kyongbum Lee, James A. Van Deventer
Summary: Synthetic cell-cell interaction systems are important for understanding multicellular communities and screening binding molecules. In this study, a previously characterized set of synthetic nanobody-antigen pairs was adapted to a yeast-bacteria coincubation format and evaluated using flow cytometry and fluorescence-activated cell sorting. The results demonstrate the efficiency of this system in evaluating ligand-target interactions and its potential for systematic characterization and high-throughput discovery of bacterial surface-binding molecules.
BIOTECHNOLOGY AND BIOENGINEERING
(2023)
Article
Biochemistry & Molecular Biology
Miok Lee, Srijana Upadhyay, Fuada Mariyam, Greg Martin, Amanuel Hailemariam, Kyongbum Lee, Arul Jayaraman, Robert S. Chapkin, Syng-Ook Lee, Stephen Safe
Summary: It has been reported that hydroxyflavones quercetin and kaempferol can bind to the NR4A1 receptor and act as antagonists in cancer cells and tumors. They inhibit pro-oncogenic NR4A1-regulated genes and pathways. This study investigated the interactions between different hydroxyflavones and the NR4A1 receptor using fluorescence and calorimetry assays. The results showed that hydroxyflavones are selective NR4A1 modulators, and some of them can be used to target patients that overexpress NR4A1.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Gastroenterology & Hepatology
Ying-Shiuan Chen, Jia Li, Rani Menon, Arul Jayaraman, Kyongbum Lee, Yun Huang, Wan Mohaiza Dashwood, Ke Zhang, Deqiang Sun, Roderick H. Dashwood
Summary: The complex interplay between gut microbes, host, and disease outcome is governed by multiple factors, with a multi-omics approach revealing the significant anti-tumor effects of spinach (SPI) in cancer prevention. SPI led to changes in gut microbiome diversity and taxonomic composition, implicating linoleate and butanoate metabolism in cancer pathways. Tumor suppression by SPI involved reshaping of the gut microbiome and alterations in host RNA-miRNA networks. Metabolomic analysis linked anticancer outcomes to linoleate bioactives and changes in glutamate and N-acetylneuraminate associated with oncogenic signaling and immune evasion. Further research on additional factors like fiber content is needed for potential interventions in at-risk patients.
Article
Medicine, Research & Experimental
Seo-Young Park, Susan Egan, Anthony J. Cura, Kathryn L. Aron, Xuankuo Xu, Mengyuan Zheng, Michael Borys, Sanchayita Ghose, Zhengjian Li, Kyongbum Lee
Summary: Reduction of monoclonal antibody disulfide bonds can impact therapeutic efficacy and safety, with potential factors including host cell proteome and stress response pathways. Analyzing Chinese hamster ovary cell protein abundances revealed distinctive characteristics in reduction-susceptible cell culture fluids, suggesting optimization of culture conditions may improve protein quality.
Article
Biotechnology & Applied Microbiology
Nicolas Huber, Edgar Alberto Alcala-Orozco, Thomas Rexer, Udo Reichl, Steffen Klamt
Summary: Cell-free production systems are commonly used for synthesizing industrial chemicals and biopharmaceuticals. This study presents a model-based optimization framework for cell-free enzyme cascades, taking into account parameter uncertainties. The approach was exemplified using the synthesis of GDP-fucose, resulting in significant improvements in the process.
METABOLIC ENGINEERING
(2024)
