期刊
METABOLIC ENGINEERING
卷 11, 期 1, 页码 64-68出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.ymben.2008.08.004
关键词
4-Coumaroylserotonin; 4-Coumaroyltyramine; Feruloylserotonin; Phenylpropanoid amides; Recombinant E. coli
资金
- Technology Development Program for Agriculture and Forestry
- Ministry of Agriculture, Forestry and Fisheries
- MOST/KOSEF [R11-2001092- 05001-0]
Plant-specific phenylpropanoid amides were produced in a recombinant Escherichia coli that expressed 4-coumarate:coenzyme A ligase from Arabidopsis and serotonin N-hydroxycinnamoyltransferase from pepper plants. Upon exogenous treatment with several precursors, high concentrations of the following phenylpropanoid amides were produced abundantly in the culture medium in a few hours: 4-coumaroylserotonin (215mg/l), 4-coumaroyloctopamine (208mg/l), and 4-coumaroyltyramine (187 mg/l). (C) 2008 Elsevier Inc. All rights reserved.
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