期刊
METABOLIC ENGINEERING
卷 10, 期 1, 页码 55-68出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.ymben.2007.08.004
关键词
Actinobacillus succinogenes; Succinate; Metabolic flux analysis; Hydrogen concentration; CO2 concentration; Pyruvate carboxylating flux; Redox balance
资金
- National Science Foundation [BES-0224596]
- Michigan State University (MSU) Research Excellence Fund
Actinobacillus succinogenes naturally produces high concentrations of succinate, a potential intermediary feedstock for bulk chemical productions. A. succinogenes responds to high CO2 and H-2 concentrations by producing more succinate and by producing less formate, acetate, and ethanol. To determine how intermediary fluxes in A. succinogenes respond to CO2 and H-2 perturbations, C-13-metabolic flux analysis was performed in batch cultures at two different NaHCO3 concentrations, with and without H-2, using a substrate mixture of [1-C-13] glucose, [U-C-13] glucose, and unlabeled NaHCO3. The resulting amino acid, organic acid, and glycogen isotopomers were analyzed by gas chromatography-mass spectrometry and NMR. In all conditions, exchange flux was observed through malic enzyme and/or oxaloacetate decarboxylase. The presence of an exchange flux between oxaloacetate, malate, and pyruvate indicates that, in addition to phosphoenolpyruvate, oxaloacetate, and malate, pyruvate is a fourth node for flux distribution between succinate and alternative fermentation products. High NaHCO3 concentrations decreased the amount of flux shunted by C-4-decarboxylating activities from the succinate-producing C-4 pathway to the formate-, acetate-, and ethanol-producing C-3 pathway. In addition, pyruvate carboxylating flux increased in response to high NaHCO3 concentrations. C-3-pathway dehydrogenase fluxes increased or decreased appropriately in response to the different redox demands imposed by the different NaHCO3 and H-2 concentrations. Overall, these metabolic flux changes allowed A. succinogenes to maintain a constant growth rate and biomass yield in all conditions. These results are discussed with respect to A. succinogenes' physiology and to metabolic engineering strategies to increase the flux to succinate. (C) 2007 Elsevier Inc. All rights reserved.
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