期刊
MEMORIAS DO INSTITUTO OSWALDO CRUZ
卷 105, 期 8, 页码 1001-1009出版社
FUNDACO OSWALDO CRUZ
DOI: 10.1590/S0074-02762010000800009
关键词
Anopheles calderoni; morphology; DNA barcodes; COI; ITS2; Colombia; Ecuador; new country records
资金
- Royal Caribbean International
- Consortium for the Barcode of Life
- Sloane Foundation
- ScholarShip Program LLC
- La Universidad del Valle, Colombia
- Mosquito Barcoding Initiative
- owner, officers
- crew
- Department of the Army
- Department of Defense
The morphologically similar taxa Anopheles calderoni, Anopheles punctimacula, Anopheles malefactor and Anopheles guarao are commonly misidentified. Isofamilies collected in Valle de Cauca, Colombia, showed morphological characters most similar to An. calderoni, a species which has never previously been reported in Colombia. Although discontinuity of the postsubcostal pale spots on the costa (C) and first radial (R-1) wing veins is purportedly diagnostic for An. calderoni, the degree of overlap of the distal postsubcostal spot on C and R-1 were variable in Colombian specimens (0.003-0.024). In addition, in 98.2% of larvae, seta 1-X was located off the saddle and seta 3-C had 4-7 branches in 86.7% of specimens examined. Correlation of DNA sequences of the second internal transcribed spacer and mtDNA cytochrome c oxidase subunit I gene (COI) barcodes (658 bp of the COI gene) generated from Colombian progeny material and wild-caught mosquitoes from Ecuador with those from the Peruvian type series of An. calderoni confirmed new country records. DNA barcodes generated for the closely related taxa, An. malefactor and An. punctimacula are also presented for the first time. Examination of museum specimens at the University of the Valle, Colombia, revealed the presence of An. calderoni in inland localities across Colombia and at elevations up to 1113 m.
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