期刊
MEMORIAS DO INSTITUTO OSWALDO CRUZ
卷 103, 期 5, 页码 501-503出版社
FUNDACO OSWALDO CRUZ
DOI: 10.1590/S0074-02762008000500017
关键词
Schistosoma mansoni; DNA extraction; microsatellites
Schistosomes are endoparasites causing a serious human disease called schistosomiasis. The quantification of parasite genetic diversity is an essential component to understand the schistosomiasis epidemiology and disease transmission patterns. In this paper, we propose a novel assay for a rapid, low costly and efficient DNA extraction method of egg, larval and adult stages of Schistosoma mansoni. One euro makes possible to perform 60,000 DNA extraction reactions at top speed (only 15 min of incubation and 5 handling steps).
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