Article
Multidisciplinary Sciences
JuKyung Lee, Han Na Suh, Saeyoung Ahn, Hye Bin Park, Jeong Yoon Lee, Hyung Jin Kim, Sang Hee Kim
Summary: This study developed electrocatalytic sensors for analyzing NADH in whole blood, which exhibited stability and a limit of detection of 3.5 μM. These sensors have the potential for extensive applications.
SCIENTIFIC REPORTS
(2022)
Article
Neurosciences
Chen Shen, Cong Chen, Tong Wang, Tong-Yao Gao, Min Zeng, Yun-Bi Lu, Wei-Ping Zhang
Summary: Nicotinamide phosphoribosyltransferase (NAMPT) is a key enzyme in the salvaging synthesis pathway of nicotinamide adenine dinucleotide (NAD). Depletion of NAMPT can lead to cognitive deficiency in hippocampus neurons and increase the risk of neurodegeneration due to mitochondrial dysfunction.
MOLECULAR NEUROBIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Min Li, Lydie Plecita-Hlavata, Evgenia Dobrinskikh, B. Alexandre McKeon, Aneta Gandjeva, Suzette Riddle, Aya Laux, Ram Raj Prasad, Sushil Kumar, Rubin M. Tuder, Hui Zhang, Cheng-Jun Hu, Kurt R. Stenmark
Summary: The study found that abnormal SIRT3 activity is related to mitochondrial dysfunction in adventitial fibroblasts from patients with pulmonary hypertension. Restoration of SIRT3 activity can reduce mitochondrial protein acetylation and restore mitochondrial function.
AMERICAN JOURNAL OF RESPIRATORY CELL AND MOLECULAR BIOLOGY
(2023)
Article
Biochemical Research Methods
Amani A. Gillette, Rebecca A. DeStefanis, Stephanie L. Pritzl, Dustin A. Deming, Melissa C. Skala
Summary: This study investigates the effects of Bcl-2 family protein inhibitors on cellular metabolism and finds that the optical redox ratio (ORR) is sensitive to changes in mitochondrial function and cell senescence, which can occur independently of cell viability. The metabolic changes caused by ABT-263 treatment can be mitigated by combination with mTORC1/2 inhibitors.
JOURNAL OF BIOMEDICAL OPTICS
(2022)
Article
Chemistry, Analytical
Jie Zhang, Zengchen Liu, Fengshou Tian, Yahong Chen
Summary: A fluorescent compound derived from coumarin and hemicyanine was synthesized and characterized in this paper, showing higher ratiometric fluorescence response activity toward NAD(P)H compared to other commonly coexisting compounds in the cell microenvironment. The fluorescence identification mechanism was deduced to be a redox reaction between the sensor and NAD(P)H. This ratiometric fluorescent probe provides a theoretical basis for sensing NAD(P)H in vitro and in vivo, and was used to build a sensitive detection platform of NAD(P)H-dependent enzyme activity based on the fluorescence method.
ANALYTICAL METHODS
(2021)
Article
Critical Care Medicine
Chenglei Su, Yan Xiao, Guozhen Zhang, Lian Liang, Hui Li, Cheng Cheng, Tao Jin, Jennifer Bradley, Mary A. Peberdy, Joseph P. Ornato, Martin J. Mangino, Wanchun Tang
Summary: This study investigated the therapeutic potential and mechanisms of exogenous nicotinamide adenine dinucleotide(+) (NAD(+)) treatment on postresuscitation myocardial and neurologic dysfunction. The results showed that NAD(+) treatment improved hemodynamic and myocardial function, reduced neurologic deficit, and increased survival rate. Furthermore, NAD(+) treatment restored ATP level and mitochondrial respiratory function, and activated the Sirtuin3 pathway.
CRITICAL CARE MEDICINE
(2022)
Article
Chemistry, Multidisciplinary
Yongwoo Jang, Taegyu Park, Eunyoung Kim, Jong Woo Park, Dong Yeop Lee, Seon Jeong Kim
Summary: By utilizing the cellular redox system, a CNT yarn supercapacitor with maximum area capacitance was developed and demonstrated excellent performance under physiological conditions. Implanted NAD/BQ/CNT yarn electrodes in a rat's abdominal cavity showed stable in vivo electrical performance of a supercapacitor. These findings suggest a promising platform for implantable energy storage devices using redox biomolecules.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2021)
Review
Nutrition & Dietetics
Andrea Benzi, Alessia Grozio, Sonia Spinelli, Laura Sturla, Andreas H. Guse, Antonio De Flora, Elena Zocchi, Joerg Heeren, Santina Bruzzone
Summary: Nicotinamide adenine dinucleotide (NAD(+)) plays a critical role in energy metabolism regulation as both a coenzyme and substrate for various enzymes. CD38, as an enzyme synthesizing Ca2+-mobilizing second messenger and a major NAD(+)-consumer, can impact NAD(+) levels. This review explores recent studies on the modulation of CD38 expression and activity, and the resulting changes in NAD(P)(H) levels in adipose tissue.
Article
Biochemistry & Molecular Biology
Cuiting Liao, Li Zhang, Rong Jiang, Juanjuan Xu, Jiarui Tang, Kai Hu, Shifang Jiang, Longhui Li, Yongqiang Yang, Jiayi Huang, Li Tang, Longjiang Li
Summary: The expression of NADK is positively correlated with APAP-induced acute liver injury, and inhibition of NADK can alleviate liver injury by increasing hepatic NAD(+) level and improving antioxidant capacity.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2022)
Article
Neurosciences
Mariajose Metcalfe, Brian T. David, Brett C. Langley, Caitlin E. Hill
Summary: Spinal cord injury leads to tissue damage and functional deficits. Administering NR can increase NAD+ levels in the injured spinal cord, protect tissue, and improve motor function.
EXPERIMENTAL NEUROLOGY
(2023)
Review
Pharmacology & Pharmacy
Yumeng Zhu, Ping Xu, Xuan Huang, Wen Shuai, Li Liu, Shuai Zhang, Rui Zhao, Xiuying Hu, Guan Wang
Summary: Nicotinamide phosphoribosyltransferase (NAMPT) plays a significant role in mammals by synthesizing nicotinamide mononucleotide (NMN) for metabolic homeostasis and cell survival, serving as a key protein in the host's defense mechanism. Neurodegenerative diseases pose a serious threat to human health, but effective drugs are limited. NAMPT has active biological functions in neurogenesis, making it a powerful therapeutic target for neurodegenerative diseases.
FRONTIERS IN PHARMACOLOGY
(2022)
Review
Nutrition & Dietetics
Qin Song, Xiaofeng Zhou, Kexin Xu, Sishi Liu, Xinqiang Zhu, Jun Yang
Summary: Nicotinamide mononucleotide (NMN) is a precursor for NAD+ biosynthesis, and supplementation has shown to increase NAD+ concentration and alleviate aging-related disorders. While most studies have been conducted in cell or animal models, several human clinical trials are currently evaluating the safety and physiological effects of NMN supplementation.
ADVANCES IN NUTRITION
(2023)
Article
Biochemical Research Methods
Ryo Matsuyama, Tomoyo Omata, Michiharu Kageyama, Ryota Nakajima, Masanobu Kanou, Kei Yamana
Summary: Nicotinamide adenine dinucleotide (NAD(+)) is an essential coenzyme for energy production. Associations between NAD(+) and aging-related diseases have been reported, and NAD(+) precursors are acknowledged as anti-aging supplements. However, there have been few studies on the link between aging or aging-related diseases and human blood NAD(+) concentration due to the instability and difficulty in measuring NAD(+). This study aimed to develop a simpler quantitative NAD(+) measurement method.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2023)
Article
Biochemistry & Molecular Biology
Chengting Luo, Changmei Yang, Xueying Wang, Yuling Chen, Xiaohui Liu, Haiteng Deng
Summary: Research showed that nicotinamide supplementation can reduce fat mass in obese mice, improve glucose tolerance, and enhance mitochondrial function in adipose tissue. Nicotinamide activates NAMPT and increases cellular NAD(+) levels to reprogram cellular metabolism for better adipose tissue function.
JOURNAL OF NUTRITIONAL BIOCHEMISTRY
(2022)
Article
Biochemistry & Molecular Biology
Cecile Cros, Marielle Margier, Helene Cannelle, Julie Charmetant, Nicolas Hulo, Laurent Laganier, Alessia Grozio, Matthias Canault
Summary: This research investigated the effects of beta-nicotinamide mononucleotide (beta-NMN) administration on macrophage phenotype and metabolism in response to peritonitis and sepsis, finding that beta-NMN improved the dysregulated inflammatory response and reduced mortality. These findings suggest that beta-NMN may be a potential therapeutic approach for peritonitis and sepsis.
FRONTIERS IN MOLECULAR BIOSCIENCES
(2022)
Article
Orthopedics
Rita E. Baumgartner, Andrew E. Federer, Evan M. Guerrero, Suhail K. Mithani, David S. Ruch, Marc J. Richard
Summary: Treatment of metacarpal fractures with low-profile plate fixation resulted in a significantly lower overall complication rate compared to traditional plating systems, providing a reliable and acceptable solution for patients.
Article
Orthopedics
Daniel J. Cunningham, Sean P. Ryan, Cierra Hong, Suhail K. Mithani, Samuel B. Adams
Summary: The study found that older age, higher comorbidity burden, diabetes, pulmonary disease, and increased number of simultaneous procedures were significantly associated with the need for flap coverage after TAA. These factors increase the risk of subsequent flap coverage following total ankle arthroplasty.
FOOT & ANKLE INTERNATIONAL
(2021)
Article
Neurosciences
Keith W. VanDusen, Yi-Ju Li, Victor Cai, Ashley Hall, Sarah Hiles, J. Will Thompson, M. Arthur Moseley, Mary Cooter, Leah Acker, Jerrold H. Levy, Kamrouz Ghadimi, Quintin J. Quinones, Michael J. Devinney, Stacey Chung, Niccolo Terrando, Eugene W. Moretti, Jeffrey N. Browndyke, Joseph P. Mathew, Miles Berger
Summary: In this study, unbiased mass spectrometry was used to identify potential neuroinflammatory pathways underlying POCD, with results demonstrating changes in complement and coagulation pathways in the CSF of POCD patients.
JOURNAL OF ALZHEIMERS DISEASE
(2021)
Article
Multidisciplinary Sciences
Niccolo Terrando, John J. Park, Michael Devinney, Cliburn Chan, Mary Cooter, Pallavi Avasarala, Joseph P. Mathew, Quintin J. Quinones, Krishna Rao Maddipati, Miles Berger
Summary: Liquid chromatography-mass spectrometry was used to analyze cerebrospinal fluid samples collected before, 24 hours after, and 6 weeks after surgery, revealing detectable levels of AA, DHA, and EPA derived lipids in human CSF, with significant postoperative changes observed in the AA metabolite pathway in centrifuged CSF pellets and supernatants.
SCIENTIFIC REPORTS
(2021)
Article
Orthopedics
Rachel E. Hein, Tyler S. Pidgeon, Suhail K. Mithani, Christopher S. Klifto, David S. Ruch, Marc J. Richard
Summary: This article reports on two complex scaphoid dislocations that demonstrate axial instability, as well as disruption of the capitohamate articulation and the long-ring metacarpal relationship. The patients underwent reduction and fixation using a wrist spanning plate, and follow-up showed maintained reduction, axial stability, and a return to painless range of motion.
JOURNAL OF HAND SURGERY-AMERICAN VOLUME
(2022)
Article
Surgery
Ronnie L. Shammas, Ji Huh, Joel Huber, Suhail K. Mithani
Summary: This study aimed to assess the factors influencing the preference for a prosthesis or hand transplantation after unilateral hand amputation. The results showed that appearance and function were the most important factors for choosing a prosthesis, while concerns about immunosuppression side effects and the intense nature of surgery and recovery were predominant for hand transplantation. Age, medical experiences, familiarity with medical issues, and religious service attendance were associated with support for hand transplantation.
ANNALS OF PLASTIC SURGERY
(2022)
Article
Anesthesiology
Jeffrey N. Browndyke, Mary C. Wright, Rosa Yang, Ayesha Syed, John Park, Ashley Hall, Katherine Martucci, Michael J. Devinney, Leslie Shaw, Teresa Waligorska, Eugene W. Moretti, Heather E. Whitson, Harvey J. Cohen, Joseph P. Mathew, Miles Berger
Summary: The study found that carriers of the APOE4 gene had stronger functional connectivity before surgery and a more significant decrease in functional connectivity after surgery, while non-carriers did not show this change. Carriers of the APOE4 gene did not show significant differences in cognitive function and CSF biomarkers compared to non-carriers, but had lower CSF A beta levels.
BRITISH JOURNAL OF ANAESTHESIA
(2021)
Article
Orthopedics
Elizabeth P. Wahl, Marc J. Richard, David S. Ruch, Suhail K. Mithani, Tyler S. Pidgeon
Summary: Most patients are willing to pay a considerable amount out of pocket for hand surgery after understanding the condition, treatment, and outcomes. Respondents are hesitant to sacrifice advanced technology despite increased costs.
JOURNAL OF BONE AND JOINT SURGERY-AMERICAN VOLUME
(2021)
Article
Surgery
Olachi O. Oleru, Neil V. Shah, Peter L. Zhou, Dillon Sedaghatpour, Jaydev B. Mistry, Bradley C. Wham, Joey Kurtzman, Suhail K. Mithani, Steven M. Koehler
Summary: This study investigated the impact of smoking on 30-day standardized outcomes following upper extremity microsurgical reconstruction. The results showed that recent smoking history was not associated with any adverse outcomes in replantation/revascularization or free flap transfer surgeries.
Article
Orthopedics
Alexander L. Lazarides, Eliana B. Saltzman, Julia D. Visgauss, Suhail K. Mithani, William C. Eward, Brian E. Brigman
Summary: The study assessed the feasibility of intraoperative indocyanine green fluorescent angiography (ICGA) for predicting wound complications in soft tissue sarcoma patients receiving preoperative radiation therapy. The results showed that ICGA had a certain level of accuracy and sensitivity in predicting wound complications.
JOURNAL OF ORTHOPAEDIC RESEARCH
(2022)
Article
Surgery
Kevin M. Klifto, David S. Ruch, Suhail K. Mithani, Tyler S. Pidgeon, Marc J. Richard, Christopher S. Klifto
Summary: Long-term, high-dose, daily proton pump inhibitors (PPI) may affect the outcomes of distal radius fractures (DRF), resulting in more median nerve injuries and radial shaft fractures, fewer contralateral upper extremity injuries, and more post-operative nonunions.
JOURNAL OF PLASTIC RECONSTRUCTIVE AND AESTHETIC SURGERY
(2022)
Article
Orthopedics
Selene G. Parekh, Fernando S. Aran, Suhail Mithani, Aman Chopra
FOOT AND ANKLE CLINICS
(2022)
Article
Orthopedics
Ramesh C. Srinivasan, Jeffrey A. Dela Cruz, Ryan D. Eubanks, Kunj K. Desai, Suhail K. Mithani, Marc J. Richard, David S. Ruch
Summary: Triangular fibrocartilage complex (TFCC) tears can cause persistent ulnar-sided wrist pain and functional loss, and surgical repair is considered when conservative treatment fails. This article describes a recently developed arthroscopic assisted ulnar foveal bone tunnel repair method, which spares dorsal structures and restores stability of the ulnar-sided wrist joint.
ARTHROSCOPY TECHNIQUES
(2022)
Article
Education, Scientific Disciplines
Jason L. Green, Kate B. Krucoff, Tracy Truong, Amie Kim, Brian J. Conway, Alexandra O. Polovneff, Kristen Rezak, Suhail K. Mithani, Paris D. Butler
Summary: This study aims to address the underrepresentation of African Americans in plastic surgery by improving the understanding of factors that may contribute to their interest in this field. The results show significant differences in demographics, specialty factors, and medical school experiences between African American medical students interested in plastic surgery and their Caucasian counterparts.
JOURNAL OF SURGICAL EDUCATION
(2023)
Article
Immunology
Nicholas A. Turner, Mollie Sweeney, Ana M. Xet-Mull, Jeremy Storm, Suhail K. Mithani, David B. Jones, Jeremy J. Miles, David M. Tobin, Jason E. Stout
Summary: Research shows that upper extremity NTM tenosynovitis cases may be caused by different organisms, with species within the Mycobacterium terrae complex increasingly reported as causes. Modern molecular speciation methods are crucial for identifying phenotypically similar NTM pathogens.
CLINICAL INFECTIOUS DISEASES
(2021)