Article
Agriculture, Dairy & Animal Science
Rafael E. Martinez, Jessica L. Leatherwood, Amanda N. Bradbery, Brittany L. Silvers, Jennifer Fridley, Carolyn E. Arnold, Erin A. Posey, Wenliang He, Fuller W. Bazer, Guoyao Wu
Summary: Our study found that regardless of age, equine enterocytes cannot convert ornithine into citrulline and arginine, which has important implications for the nutrition, performance, and health of horses.
JOURNAL OF ANIMAL SCIENCE
(2022)
Article
Biochemistry & Molecular Biology
Karolina A. P. Wijnands, Dennis M. Meesters, Benjamin Vandendriessche, Jacob J. Briede, Hans M. H. van Eijk, Peter Brouckaert, Anje Cauwels, Wouter H. Lamers, Martijn Poeze
Summary: Competition between NOS3 and NOS2 for arginine during endotoxemia appears crucial in the derangement of microcirculatory flow. This study found that L-citrulline supplementation enhances de novo arginine synthesis and NO production in mice during endotoxemia, especially in mice with functional NOS3-enzyme.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Immunology
Melanie C. McKell, Rebecca R. Crowther, Stephanie M. Schmidt, Michelle C. Robillard, Rachel Cantrell, Maria A. Lehn, Edith M. Janssen, Joseph E. Qualls
Summary: Macrophages rely on L-arginine for anti-Mtb defense, not only for NO production but also for regulating metabolism and other host defense mechanisms. Inhibition of glycolysis or mTOR reduces macrophage control of Mtb, as well as macrophage number and viability in the presence of L-arginine. L-arginine is essential for macrophage function in combating intracellular pathogens.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Kei Yamamoto, Yosuke Masakari, Yasuko Araki, Atsushi Ichiyanagi, Kotaro Ito
Summary: This study aimed to develop an enzyme that could specifically detect citrulline and successfully improved the specific dehydrogenase activity by introducing a mutation. The enzymatic detection system using this enzyme can effectively detect citrulline in human blood.
Article
Agriculture, Dairy & Animal Science
Hiep Thi Dao, Nishchal K. Sharma, Emma J. Bradbury, Robert A. Swick
Summary: The study examined the effects of reduced protein diet, arginine deficiency, and the addition of arginine substitutes on Hy-Line Brown laying hens. It was found that while an arginine deficient diet decreased performance, the addition of guanidinoacetic acid or citrulline allowed birds to perform similarly or better than with added arginine.
Article
Agriculture, Dairy & Animal Science
Hiep Thi Dao, Nishchal K. Sharma, Emma J. Bradbury, Robert A. Swick
Summary: Supplementing broilers with arginine and citrulline may improve performance under warm temperature conditions. Feeding broilers with normal protein diets is necessary to maintain growth performance.
Article
Nutrition & Dietetics
Maciej Tyszko, Anna Lemanska-Perek, Jakub Smiechowicz, Paulina Tomaszewska, Przemyslaw Biecek, Waldemar Gozdzik, Barbara Adamik
Summary: Gastrointestinal (GI) failure in sepsis can be indicated by changes in biomarkers such as citrulline and I-FABP. Lower citrulline concentrations were observed in septic shock patients compared to non-shock patients. In addition, higher levels of I-FABP were found in septic shock patients on certain days. Patients with GI failure had lower citrulline levels and higher AGI scores, which were associated with increased 28-day mortality. These results suggest that citrulline measurements and AGI assessment have clinical potential in monitoring GI function and integrity in sepsis.
Article
Biochemistry & Molecular Biology
Yu Chen, Shidong Liu, Caiyan Wan, Guangxian Zhang
Summary: The novel flame retardant ACPMPA, synthesized based on L-citrulline, demonstrated enhanced flame retardancy and durability for cotton fabrics. The treated cotton maintained high LOI even after multiple laundry cycles, showing excellent flame retardant properties with minimal release of flammable volatiles at high temperatures. Additionally, the treated cotton fabric showed no structural changes, no free formaldehyde, and retained good tensile strength and whiteness.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2021)
Article
Engineering, Chemical
Zaiwei Man, Jin Li, Huihui Cui, Zhiqiang Cai, Jing Guo
Summary: This study optimized the L-citrulline biosynthesis operon in C. glutamicum and successfully constructed an efficient L-citrulline-producing strain. The L-citrulline production was further improved by plasmid-based overexpression of the optimized operon.
Article
Nutrition & Dietetics
Tongtong Ba, Dai Zhao, Yiqin Chen, Cuiping Zeng, Cheng Zhang, Sai Niu, Hanchuan Dai
Summary: This study investigated the mechanism of action of L-cit in alleviating oxidative damage and immune dysfunction caused by iron overload in the thymus. The results showed that L-cit could reduce thymus histological damage and iron deposition, while increasing CD8(+) T lymphocyte number and antioxidative capacity. Furthermore, the NF-kappa B pathway, NCOA4-mediated ferritinophagy, and ferroptosis were attenuated.
Article
Agriculture, Dairy & Animal Science
Victoria Anthony Uyanga, Lei Liu, Jingpeng Zhao, Xiaojuan Wang, Hongchao Jiao, Hai Lin
Summary: This study investigates the influence of L-citrulline (L-Cit) on body temperature, nitric oxide production, and inflammatory responses. The results reveal dose-dependent effects of L-Cit administration on these parameters and suggest that both central and peripheral administration of L-Cit can induce changes in body temperature, nitric oxide concentration, and inflammatory milieu.
Article
Obstetrics & Gynecology
Laura Ormesher, Stephanie A. Worton, Ashley Best, Susanna R. Dodd, Alice Dempsey, Elizabeth C. Cottrell, Heather Glossop, Catherine Chmiel, Hoi Yee Wu, Ben Hardwick, Sophie Hennessy, Edward D. Johnstone, Jenny E. Myers
Summary: Oral supplementation with L-citrulline is acceptable and increases citrulline and arginine levels in pregnant women with chronic hypertension. However, this study did not find any significant effects of L-citrulline on blood pressure and other vascular outcomes. Further research is needed to understand the pharmacokinetic changes of L-citrulline during pregnancy.
REPRODUCTIVE SCIENCES
(2023)
Article
Nutrition & Dietetics
Stefano Maric, Pascal Fluchter, Laura Chiara Guglielmetti, Ralph Fabian Staerkle, Tom Sasse, Tanja Restin, Christoph Schneider, Stefan Gerhard Holland-Cunz, Pascal Crenn, Raphael Nicolas Vuille-dit-Bille
Summary: This study revealed a correlation between small intestinal basolateral LAT4 expression and plasma citrulline concentration for the first time, indicating LAT4's important role in mediating citrulline efflux from enterocytes. Additionally, urine arginine was found to correlate with plasma citrulline, suggesting it as a potential additional marker for small intestine enterocyte mass. Furthermore, the expression of basolateral LAT4 along the human small intestine was demonstrated for the first time.
CLINICAL NUTRITION
(2021)
Article
Food Science & Technology
Monica Volino-Souza, Gustavo Vieira de Oliveira, Otavio do Couto Vellozo, Carlos Adam Conte-Junior, Thiago da Silveira Alvares
Summary: The study found that the microencapsulated L-citrulline in watermelon rind and nitrate in beetroot remained stable throughout storage, exhibiting good stability over time.
JOURNAL OF FOOD SCIENCE AND TECHNOLOGY-MYSORE
(2021)
Article
Oncology
Silvia De Pietri, Sarah Weischendorff, Mathias Rathe, Thomas Leth Frandsen, Henrik Hasle, Jacob Nersting, Claus H. Nielsen, Claus Moser, Klaus Mueller
Summary: Chemotherapy-induced mucositis increases the risk of blood stream infections (BSI). Our study found that patients who developed BSI had a larger decrease in plasma citrulline and higher levels of CCL20, suggesting more severe intestinal mucositis. These markers may be useful in early risk stratification for treatment decisions.
INTERNATIONAL JOURNAL OF CANCER
(2023)
Article
Nutrition & Dietetics
Aaron Hengist, Russell G. Davies, Peter J. Rogers, Jeff M. Brunstrom, Luc J. C. van Loon, Jean-Philippe Walhin, Dylan Thompson, Francoise Koumanov, James A. Betts, Javier T. Gonzalez
Summary: Restricting sugar or total dietary carbohydrate does not modulate physical activity level or energy intake when energy density is controlled for.
EUROPEAN JOURNAL OF NUTRITION
(2023)
Article
Nutrition & Dietetics
Floris K. Hendriks, Jeffrey H. W. Kuijpers, Janneau M. X. van Kranenburg, Joan M. G. Senden, Frank M. Van der Sande, Jeroen P. Kooman, Steven J. R. Meex, Luc J. C. van Loon
Summary: This study recruited 10 ESRD patients for a randomized crossover trial and found that protein ingestion and exercise during hemodialysis did not compromise the removal of uremic toxins.
JOURNAL OF RENAL NUTRITION
(2023)
Article
Endocrinology & Metabolism
Luuk Hilkens, Marleen Boerboom, Nick van Schijndel, Judith Bons, Luc J. C. van Loon, Jan-Willem van Dijk
Summary: This study examined whether collagen supplementation enhances the effects of high-impact exercise on bone metabolism and whether increasing exercise frequency provides greater benefits. The results showed that 5 minutes of daily high-impact exercise can stimulate bone formation, but collagen supplementation or increased exercise frequency does not further increase bone formation.
Article
Geriatrics & Gerontology
Cas J. Fuchs, Remco Kuipers, Jan A. Rombouts, Kim Brouwers, Vera B. Schrauwen-Hinderling, Joachim E. Wildberger, Lex B. Verdijk, Luc J. C. van Loon
Summary: This study assessed the differences in muscle group volumes between young and older males and found that the most significant differences were observed in the thigh muscle group, with the quadriceps femoris showing the largest difference in muscle volume between young and older men. Overall, young men have larger muscle volumes compared to older men.
EXPERIMENTAL GERONTOLOGY
(2023)
Article
Endocrinology & Metabolism
Maarten Overkamp, Lisanne H. P. Houben, Thorben Aussieker, Janneau M. X. van Kranenburg, Philippe J. M. Pinckaers, Ulla R. Mikkelsen, Milou Beelen, Sandra Beijer, Luc J. C. van Loon, Tim Snijders
Summary: ADT reduces skeletal muscle mass in prostate cancer patients, but resistance exercise training can offset this effect.
JOURNAL OF CLINICAL ENDOCRINOLOGY & METABOLISM
(2023)
Article
Neurosciences
Heather L. Petrick, Rachel M. Handy, Bayley Vachon, Sara M. Frangos, Andrew M. Holwerda, Annemarie P. Gijsen, Joan M. Senden, Luc J. C. van Loon, Graham P. Holloway
Summary: Muscle disuse decreases muscle protein synthesis rates and induces atrophy, while nitrate supplementation can improve mitochondrial function. This study found that nitrate intake could prevent the decline in mitochondrial function caused by muscle disuse, but it was not able to prevent muscle atrophy.
JOURNAL OF PHYSIOLOGY-LONDON
(2023)
Article
Biochemistry & Molecular Biology
Anne G. W. E. Wintjens, Peter-Paul K. H. Fransen, Kaatje Lenaerts, Hong Liu, Geert C. van Almen, Sebastiaan van Steensel, Marion J. Gijbels, Ignace H. J. T. de Hingh, Patricia Y. W. Dankers, Nicole D. Bouvy
Summary: A pH-sensitive supramolecular hydrogel is developed as a drug delivery vehicle to increase the intraperitoneal retention time of specific drugs. In vitro, postmortem, and in vivo experiments demonstrate the feasibility, safety, and tissue compatibility of the hydrogel. The study concludes that the supramolecular hydrogel is safe and shows good tissue compatibility for intraperitoneal application in rats.
MACROMOLECULAR BIOSCIENCE
(2023)
Article
Sport Sciences
Thorben Aussieker, Luuk Hilkens, Andrew m. Holwerda, Cas j. Fuchs, Lisanne h. p. Houben, Joan m. Senden, Jan-willem VAN Dijk, Tim Snijders, Luc j. c. VAN Loon
Summary: This study assessed the impact of whey protein and collagen protein on postexercise protein synthesis rates. The results showed that whey protein increased myofibrillar protein synthesis rates, but had no effect on muscle connective protein synthesis rates. Collagen protein did not further increase muscle connective protein synthesis rates during the early stages of postexercise recovery in recreational athletes.
MEDICINE & SCIENCE IN SPORTS & EXERCISE
(2023)
Article
Sport Sciences
Jorn Trommelen, Glenn A. A. van Lieshout, Pardeep Pabla, Jean Nyakayiru, Floris K. K. Hendriks, Joan M. M. Senden, Joy P. B. Goessens, Janneau M. X. van Kranenburg, Annemie P. P. Gijsen, Lex B. B. Verdijk, Lisette C. P. G. M. de Groot, Luc J. C. van Loon
Summary: Pre-sleep ingestion of casein and whey protein increases overnight muscle protein synthesis rates after exercise.
Article
Immunology
Min Deng, Merel R. Aberle, Annemarie A. J. H. M. van Bijnen, Gregory van der Kroft, Kaatje Lenaerts, Ulf P. Neumann, Georg Wiltberger, Frank G. Schaap, Steven W. M. Olde Damink, Sander S. Rensen
Summary: This study found that high levels of lipocalin-2 are associated with neutrophil activation in patients with pancreatic cancer cachexia and may contribute to their poor nutritional status.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Nutrition & Dietetics
Devin G. McCarthy, Jack Bone, Matthew Fong, Phillippe J. M. Pinckaers, William Bostad, Douglas L. Richards, Luc J. C. van Loon, Martin J. Gibala
Summary: The effect of acute ketone monoester (KE) supplementation on exercise performance is unclear. This study aimed to investigate the difference in mean power output during a 20-min cycling time trial (TT) after KE ingestion compared to a placebo (PL). Twenty-three trained cyclists completed two trials with either KE or PL ingestion. The results showed that mean TT power output was lower after KE ingestion compared to PL, suggesting impaired performance.
INTERNATIONAL JOURNAL OF SPORT NUTRITION AND EXERCISE METABOLISM
(2023)
Article
Nutrition & Dietetics
Glenn A. A. van Lieshout, Jorn Trommelen, Jean Nyakayiru, Janneau van Kranenburg, Joan M. Senden, Lex B. Verdijk, Luc J. C. van Loon
Summary: This study assessed the postprandial plasma amino acid concentrations after ingestion of protein in either solid or liquid form. The results showed that there was no difference in the availability of amino acids after consuming protein as a bar or as a drink in healthy adult females.
INTERNATIONAL JOURNAL OF SPORT NUTRITION AND EXERCISE METABOLISM
(2023)
Article
Medicine, General & Internal
Annet A. M. Duivenvoorden, Mathias Clarysse, Laurens J. Ceulemans, Robert H. Geelkerken, Joep P. M. Derikx, Jean-Paul P. M. de Vries, Hessel C. J. L. Buscher, Steven W. M. Olde Damink, Frederik Jan van Schooten, Tim Lubbers, Kaatje Lenaerts
Summary: This study aims to validate a panel of plasma biomarkers and investigate volatile organic compound (VOC) profiles in exhaled air as a tool to timely and accurately diagnose Acute Mesenteric Ischaemia (AMI). 120 patients with clinical suspicion of AMI will be recruited, and their plasma levels and VOCs in exhaled air will be assessed. The diagnosis of AMI will be based on CT, endovascular and surgical reports, clinical findings, and verified by histopathological examination if applicable.
Article
Biology
Danique J. I. Heuvelings, Anne G. W. E. Wintjens, Audrey C. H. M. Jongen, Maurits J. C. A. M. Gielen, Kaatje Lenaerts, Peter-Paul K. H. Fransen, Marion J. Gijbels, Geert C. van Almen, Patricia Y. W. Dankers, Ignace H. J. T. de Hingh, Nicole D. Bouvy
Summary: This study evaluates the effect of intraperitoneal hydrogel administration on anastomotic healing. The results suggest that the injection of the hydrogel may not be safe and warrants further optimization.
Article
Nutrition & Dietetics
Gabriel Nasri Marzuca-Nassr, Andrea Alegria-Molina, Yuri SanMartin-Calisto, Macarena Artigas-Arias, Nolberto Huard, Jorge Sapunar, Luis A. Salazar, Lex B. Verdijk, Luc J. C. van Loon
Summary: Resistance exercise training can effectively increase muscle mass and function in older adults. This study compared the impact of resistance exercise training on muscle mass and function in older adults aged 65-75 years versus those above 85 years, and found no differences between the two groups.
INTERNATIONAL JOURNAL OF SPORT NUTRITION AND EXERCISE METABOLISM
(2023)