Hormone Replacement Therapy and Messenger RNA Expression of Estrogen Receptor Coregulators after Exercise in Postmenopausal Women

DIELI-CONWRIGHT, C. M., T. M. SPEKTOR, J. C. RICE, F. R. SATTLER, and E. T. SCHROEDER. Hormone Replacement Therapy and Messenger RNA Expression of Estrogen Receptor Coregulators after Exercise in Postmenopausal Women. Med. Sci. Sports Exerc., Vol. 42, No. 3, pp. 422-429, 2010. The use of hormone replacement therapy (HRT) is a potential treatment to relieve symptoms of menopause in postmenopausal women; however, the effects on skeletal muscle are unclear. Specifically, it is unknown if HRT enhances estrogen receptor (ER) transcriptional activation in skeletal muscle at rest and after resistance exercise. Purpose: To evaluate changes in the messenger RNA (mRNA) expression of ER coregulators ( steroid receptor coactivator-1 (SRC-1) and silencing mediator of retinoid and thyroid receptors (SMRT)) in postmenopausal women after a maximal eccentric exercise bout. Methods: Fourteen postmenopausal women were divided into two groups: Control, women not using HRT (n = 6, 59.2 +/- 4.2 yr, 63.1 +/- 17.4 kg); or HRT, women using traditional HRT (n = 8, 58.5 +/- 3.7 yr, 89.5 +/- 23.7 kg). Participants performed 10 sets of 10 maximal eccentric repetitions of single-leg extension on the Cybex dynamometer at 60 degrees.s(-1) with 20-s rest periods between sets. Muscle biopsies of the vastus lateralis were obtained from the exercised leg at baseline and 4 h after the exercise bout. mRNA expression was determined using real-time polymerase chain reaction for SRC-1 and SMRT. Results: mRNA expression of SRC-1 significantly increased (P <= 0.01; 2.4- to 5.2-fold change) and mRNA expression of SMRT significantly decreased (P <= 0.01; -1.3- to -4.3-fold change) after the exercise bout in both groups. We observed significantly greater changes in mRNA expression of SRC-1 and SMRT (P <= 0.01) in the HRT group compared with controls after exercise. Conclusions: A single bout of maximal eccentric exercise enhances ER transcriptional activity with a greater response present in postmenopausal women using HRT.