Article
Biochemistry & Molecular Biology
Ryan A. Denu, Peiman Hematti
Summary: MSCs have great potential for cellular therapy but are limited by tissue scarcity, requiring ex vivo expansion. Expansion and aging are associated with oxidative stress, highlighting the need for strategies to reduce it.
FREE RADICAL BIOLOGY AND MEDICINE
(2021)
Article
Biochemistry & Molecular Biology
Matthew T. Shephard, Marwan M. Merkhan, Nicholas R. Forsyth
Summary: The secretome of human mesenchymal stem cells (hMSC) has various effects that can be used for therapeutic purposes. Previous studies have shown that the composition of the secretome can be influenced by low oxygen conditions. In this study, using the Jurkat T cell model, it was found that cell growth was higher in serum-free media compared to serum-free conditioned media (SFCM), and SFCM suppressed the secretion of IL-2 while serum-free media supported its release. IL-10 was identified as playing an immunosuppressive role by reducing IL-2 secretion.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Virology
Mark A. A. Harrison, Emily M. Hochreiner, Brooke P. Benjamin, Sean E. Lawler, Kevin J. Zwezdaryk
Summary: This study revealed a potential role of human cytomegalovirus (HCMV) in glioblastoma (GBM). The findings demonstrated that HCMV infection can induce metabolic reprogramming in GBM cells, leading to increased tumor progression. Furthermore, the study revealed that the secretome of HCMV-infected GBM cells can alter the expression of key metabolic proteins and epigenetic markers in uninfected stromal cells, further promoting tumor development.
Review
Biochemistry & Molecular Biology
Yoojin Seo, Min-Jung Kang, Hyung-Sik Kim
Summary: MSCs are used as cell therapeutics for immune disorders due to their immunoregulatory properties, but variation among cells from different donors and rapid clearance after transplantation limit their efficacy. Various bioengineering strategies, including genetic modification and physical engineering, have been suggested to improve the immunomodulatory functions of MSCs.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Cell & Tissue Engineering
Yolande F. M. Ramos, Tobias Tertel, Georgina Shaw, Simon Staubach, Rodrigo Coutinho de Almeida, Eka Suchiman, Thomas B. Kuipers, Hailiang Mei, Frank Barry, Mary Murphy, Bernd Giebel, Ingrid Meulenbelt
Summary: This study investigates the similarities and therapeutic potential of hiMSCs and hBMSCs. RNA sequencing reveals a high resemblance between the two, with both cell types exhibiting immunomodulatory properties. hiMSCs stand out in terms of the expression and secretion of immunomodulatory genes.
STEM CELL RESEARCH & THERAPY
(2022)
Article
Neurosciences
Francesca Pischiutta, Enrico Caruso, Helena Cavaleiro, Antonio J. Salgado, David J. Loane, Elisa R. Zanier
Summary: Traumatic brain injury (TBI) patients require better neuroprotective/neuroreparative strategies. Mesenchymal stromal cells (MSCs) have shown promise in neuroprotection through paracrine mechanisms. Immunomodulation by MSC-derived secretome is a key mechanism for their therapeutic effects. Secretome promotes tissue repair and neurological recovery, but excessive neuroinflammatory response can worsen TBI.
EXPERIMENTAL NEUROLOGY
(2022)
Article
Oncology
Federico Villa, Silvia Bruno, Ambra Costa, Mingchuan Li, Michele Russo, James Cimino, Paola Altieri, Clarissa Ruggeri, Cansu Gorgun, Pierangela De Biasio, Dario Paladini, Domenico Coviello, Rodolfo Quarto, Pietro Ameri, Alessandra Ghigo, Silvia Ravera, Roberta Tasso, Sveva Bollini
Summary: This study evaluated and compared the cardioprotective effects of secretomes secreted by adult and fetal human stem cells, showing that both could protect against Dox-induced cardiac cell damage, reducing cardiotoxicity.
Article
Cardiac & Cardiovascular Systems
Masayuki Takano, Hidekazu Kondo, Taisuke Harada, Masaki Takahashi, Yumi Ishii, Hirochika Yamasaki, Tong Shan, Kumiko Akiyoshi, Takashi Shuto, Yasushi Teshima, Tomoyuki Wada, Kunio Yufu, Hidenori Sako, Hirofumi Anai, Shinji Miyamoto, Naohiko Takahashi
Summary: The SGLT2 inhibitor empagliflozin directly affects human epicardial adipocytes and preadipocytes, reducing adipogenesis and improving the paracrine secretome profile of epicardial adipose tissue (EAT), potentially through the regulation of IL-6 expression.
JACC-BASIC TO TRANSLATIONAL SCIENCE
(2023)
Article
Cell Biology
Abhilok Garg, Sheeba Khan, N. Luu, Davies J. J. Nicholas, Victoria Day, Andrew L. L. King, Janine Fear, Patricia F. F. Lalor, Philip N. N. Newsome
Summary: Priming human MSCs with TGF beta(1) can enhance their effectiveness in treating liver injury by improving their homing and reducing inflammation.
JOURNAL OF CELLULAR AND MOLECULAR MEDICINE
(2023)
Article
Immunology
Elise C. Wilcox, Elazer R. Edelman
Summary: Endothelial cells interact with and regulate the immune system, modulating immune cell function through the expression of various molecules and influencing immune recognition outcomes. Cell-substratum interactions impact endothelial cell phenotype and subsequently affect the immune response to transplanted cells. Endothelial cells grown on different substrata exhibit distinct basal expression levels and responsiveness to inflammatory stimuli. Understanding the relationship between endothelial cell substratum and allorecognition is crucial for predicting and modifying the outcome of endothelial cell-immune interactions.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Biology
Sunisa Yoodee, Chadanat Noonin, Kanyarat Sueksakit, Rattiyaporn Kanlaya, Sakdithep Chaiyarit, Paleerath Peerapen, Visith Thongboonkerd
Summary: Using label-free quantitative proteomics, this study identified significantly altered secreted proteins from COM-exposed macrophages, suggesting their potential role in renal fibrogenesis in kidney stone disease.
COMMUNICATIONS BIOLOGY
(2021)
Article
Obstetrics & Gynecology
Alice Luddi, Valentina Pavone, Laura Governini, Angela Capaldo, Claudia Landi, Francesca Ietta, Eugenio Paccagnini, Giuseppe Morgante, Vincenzo De Leo, Paola Piomboni
Summary: The study demonstrates that the secretome of implanting embryos can influence the molecular profile of endometrial organoids, increase the relative abundance of specific protein GdA, and enhance clinical pregnancy rates. Using embryo-conditioned culture medium during embryo transfer can effectively improve in vitro fertilization outcomes.
FERTILITY AND STERILITY
(2021)
Article
Multidisciplinary Sciences
Marek Kukumberg, Tatsanee Phermthai, Suparat Wichitwiengrat, Xiaoyuan Wang, Subramanian Arjunan, Suet Yen Chong, Chui-Yee Fong, Jiong-Wei Wang, Abdul Jalil Rufaihah, Citra Nurfarah Zaini Mattar
Summary: The study found that secretome derived from human amniotic fluid stem cells (AFSC-S) cultured under hypoxic conditions has higher concentrations of soluble bioactive factors, which positively affect cardioprotection and cell proliferation. Experimental validation showed that hypoxic-generated AFSC-S has potential therapeutic effects in ischemic cardiovascular disease through regenerative medicine.
SCIENTIFIC REPORTS
(2021)
Article
Immunology
Furkan Yigitbilek, Elif Ozdogan, Nitin Abrol, Walter D. Park, Michael J. Hansen, Surendra Dasari, Mark D. Stegall, Timucin Taner
Summary: Liver-resident mesenchymal stem cells (L-MSCs) are more potent inhibitors of NK cell functions compared to their counterparts, contributing to the tolerogenic liver microenvironment and liver-induced systemic tolerance.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Multidisciplinary Sciences
Rajkaran Banga, Veerkaran Banga, Amr Eltalla, Lauren Shahin, Sonam Parag, Maha Naim, Easha Iyer, Neha Kumrah, Brian Zacharias, Lubov Nathanson, Vladimir Beljanski
Summary: This study investigates how the addition of tamoxifen (TX) and chloroquine (CQ) alters the levels of long non-coding RNAs (lncRNAs) and evaluates their impact on the immunomodulatory properties of MIAMI cells. The findings suggest the potential involvement of multiple lncRNAs in modulating the response of MIAMI cells to inflammation.
Article
Endocrinology & Metabolism
Margherita Maffei, Antonio Giordano
Summary: Leptin, produced by white adipose tissue, plays a crucial role in informing the brain about energy stores and regulating energy balance. Obesity-induced alterations in certain hubs of the hypothalamus may hinder the action of Leptin, leading to resistance in obese patients. Understanding how Leptin gains access to the brain and how neuronal plasticity interferes with its function may provide insights for enhancing the effects of Leptin.
REVIEWS IN ENDOCRINE & METABOLIC DISORDERS
(2022)
Review
Oncology
Antonio Melillo, Andrea Chirico, Giuseppe De Pietro, Luigi Gallo, Giuseppe Caggianese, Daniela Barone, Michelino De Laurentiis, Antonio Giordano
Summary: This review article is the first to assess the impact of virtual reality on the rehabilitation care of cancer survivors. It suggests that virtual reality rehabilitation systems may improve adherence to training programs and better meet the needs of cancer patients. However, more data and randomized controlled trials are needed to confirm this hypothesis.
Review
Cell Biology
Pierluigi Scalia, Stephen J. Williams, Yoko Fujita-Yamaguchi, Antonio Giordano
Summary: The IGFs are important growth factors that play a key role in cell growth and cell cycle progression through their cellular receptors. Understanding the intracellular signaling mechanisms of IGFs is crucial for cancer research. This study provides a comprehensive summary of the current understanding of the regulatory mechanisms of IGFs in cancer and suggests new perspectives for further research.
Article
Anatomy & Morphology
Ilenia Severi, Silvia Abbatelli, Jessica Perugini, Eleonora Di Mercurio, Martina Senzacqua, Antonio Giordano
Summary: This study is the first comprehensive morphological investigation into the distribution and physiological role of BChE in the mouse gastrointestinal tract. BChE-positive cells were found in various digestive organs, such as the stomach, liver, and intestine, and were closely linked to ghrelin production.
JOURNAL OF ANATOMY
(2023)
Article
Medicine, General & Internal
Antonio Tufano, Nadia Cordua, Valerio Nardone, Raffaele Ranavolo, Rocco Simone Flammia, Federica D'Antonio, Federica Borea, Umberto Anceschi, Costantino Leonardo, Andrea Morrione, Antonio Giordano
Summary: This study investigated the prognostic value of site-specific metastases in patients with metastatic upper tract urothelial carcinoma (mUTUC) and its association with survival outcomes. The results showed that distant lymph nodes were the most common site of single-organ metastasis in mUTUC patients. Patients with liver metastases and multiple organ metastases had worse survival outcomes. Additionally, chemotherapy and radical nephroureterectomy (RNU) were identified as predictors of better survival outcomes in mUTUC patients.
JOURNAL OF CLINICAL MEDICINE
(2022)
Article
Oncology
Marcella Barbarino, Maria Bottaro, Laura Spagnoletti, Maria Margherita de Santi, Raffaella Guazzo, Chiara Defraia, Cosimo Custoza, Gabriella Serio, Francesco Iannelli, Matilde Pesetti, Raffaele Aiello, Diletta Rosati, Edoardo Zanfrini, Luca Luzzi, Cristiana Bellan, Antonio Giordano
Summary: Primary cilium is a cellular organelle found on almost all mammalian cells, which transduces signals and regulates various physiological pathways. Dysfunction in primary cilium is associated with pathologic conditions including cancer. This study focused on the role of primary cilium in the Hedgehog signaling pathway in malignant pleural mesothelioma (MPM) and uncovered evidence of a PC-independent regulation of Hh signaling in MPM.
Article
Cell Biology
Antonio C. Pagano Zottola, Ilenia Severi, Astrid Cannich, Philippe Ciofi, Daniela Cota, Giovanni Marsicano, Antonio Giordano, Luigi Bellocchio
Summary: By activating the CB1 receptor, cannabinoids regulate mitochondrial physiology and energy metabolism in adipocytes through intracellular signaling pathways.
Article
Oncology
Antonella Fameli, Valerio Nardone, Mojtaba Shekarkar Azgomi, Giovanna Bianco, Claudia Gandolfo, Bianca Maria Oliva, Marika Monoriti, Rita Emilena Saladino, Antonella Falzea, Caterina Romeo, Natale Daniele Calandruccio, Domenico Azzarello, Rocco Giannicola, Luigi Pirtoli, Antonio Giordano, Pierfrancesco Tassone, Pierosandro Tagliaferri, Maria Grazia Cusi, Luciano Mutti, Cirino Botta, Pierpaolo Correale
Summary: Peripheral immune-checkpoint blockade with PD-1/PD-L1 antibodies is a promising treatment strategy for mNSCLC patients. This study investigates the impact of PD-1/PD-L1 blockade on specific immune cell subsets and suggests a correlation between immune subpopulation deregulation and autoimmunity.
FRONTIERS IN ONCOLOGY
(2022)
Review
Microbiology
Elena Rossi, Luciano Mutti, Andrea Morrione, Antonio Giordano
Summary: SARS-CoV-2 infection can lead to interstitial pneumonia and neurological symptoms, especially in elderly patients prone to cytokine storm and severe outcomes like acute respiratory distress syndrome. Limited understanding exists on the correlation between symptoms and clinical outcomes, as well as virus-host interactions.
Review
Biochemistry & Molecular Biology
Giovanni Ciccarelli, Stefano Conte, Giovanni Cimmino, Patrizia Maiorano, Andrea Morrione, Antonio Giordano
Summary: Atherosclerosis is a complex disease involving metabolic processes and inflammation. Despite improved therapies, a significant residual risk remains due to unmet therapeutic targets. Endothelial dysfunction and lipid infiltration are primary causes of atherosclerotic plaque progression. Mitochondrial dysfunction can affect arterial wall cells, leading to oxidative stress, chronic inflammation, and intracellular lipid deposition. Evaluating mitochondrial defects through the detection and characterization of mitochondrial DNA (mtDNA) is crucial, and mitochondria-targeting therapies show promise in treating atherosclerosis.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Oncology
Elisa Ventura, Giacomo Ducci, Reyes Benot Dominguez, Valentina Ruggiero, Antonino Belfiore, Elena Sacco, Marco Vanoni, Renato V. V. Iozzo, Antonio Giordano, Andrea Morrione
Summary: Progranulin plays an important pro-tumorigenic role in various solid tumors and has diagnostic and prognostic values in multiple tumor types. The molecular mechanisms underlying progranulin's function in cancer are not fully understood, but recent evidence suggests a strong context-dependency of progranulin signaling. This review focuses on summarizing the current evidence supporting the pro-oncogenic role of progranulin and its molecular mechanisms of action in cancer.
Review
Agriculture, Dairy & Animal Science
Giovanna Liguori, Anna Costagliola, Renato Lombardi, Orlando Paciello, Antonio Giordano
Summary: Animal-assisted interventions (AAIs) are planned activities carried out in multidisciplinary teams with educational, therapeutic, and recreational purposes. These interventions offer many advantages to subjects but may expose them to zoonotic-pathogen transmission. Therefore, ensuring positive animal welfare and implementing preventive measures are crucial for the safety of patients.
Review
Biochemistry & Molecular Biology
Alfredo Caturano, Margherita D'Angelo, Andrea Mormone, Vincenzo Russo, Maria Pina Mollica, Teresa Salvatore, Raffaele Galiero, Luca Rinaldi, Erica Vetrano, Raffaele Marfella, Marcellino Monda, Antonio Giordano, Ferdinando Carlo Sasso
Summary: Oxidative stress plays a critical role in the development and progression of diabetes and its associated complications. The imbalance between reactive oxygen species (ROS) production and the body's antioxidant defense mechanisms leads to cellular damage and dysfunction. By understanding and addressing oxidative stress, it may be possible to alleviate the burden of diabetes and improve patient outcomes.
CURRENT ISSUES IN MOLECULAR BIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Anna Valentino, Stefania Leuci, Umberto Galderisi, Gianrico Spagnuolo, Michele Davide Mignogna, Gianfranco Peluso, Anna Calarco
Summary: We have found a different miRNA expression profile in plasma-derived exosomes (P-EVs) from MPV patients compared to healthy controls, with a significant downregulation of miR-148a-3p in MPV patients. Bioinformatics analysis predicted MMP7 as a potential target of miR-148a-3p. In vitro experiments showed that treatment with Dsg3 autoantibodies downregulated miR-148a-3p and upregulated MMP7 expression, leading to cell dissociation, which was prevented by overexpression of miR-148a-3p.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Letter
Oncology
Pierpaolo Correale, Francesca Pentimalli, Valerio Nardone, Antonio Giordano, Luciano Mutti