Article
Biochemistry & Molecular Biology
Yana Zhao, Huizhen Li, Zhijun Zhang, Zhiqing Ren, Fuhan Yang
Summary: Polyphenols, particularly RosA and CafA, were extracted from Perilla frutescens using ultrasonic-assisted ethanol extraction and ultrasound-assisted cellulase hydrolysis methods. The total phenolic content was significantly increased by cellulase hydrolysis, and further purification led to higher phenolic content. HPLC analysis revealed the composition of polyphenols, and a system for isolating high-purity RosA and CafA was established. Antibacterial activities of the polyphenols demonstrated effective inhibition of various bacteria.
Article
Spectroscopy
Wensong Yao, Xiaomin Zhang, Zhen Lin
Summary: The study demonstrates that sodium molybdate significantly enhances the chemiluminescent reaction between hydrogen peroxide and luminol, providing a convenient and sensitive method for hydrogen peroxide determination, as well as potential application for glucose detection when combined with glucose oxidase. This finding suggests promising applications of sodium molybdate in analytical chemistry.
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
(2022)
Article
Chemistry, Multidisciplinary
Xin Zhang, Yanqiao Zhao, Bin Li, Meichen Guo, Jinwu Lv, Yuantao Wei
Summary: The effects of three extraction methods (ultrasonic-assisted water extraction, ultrasonic-assisted ethanol extraction, and ultrasonic-assisted aqueous two-phase extraction) on the yields, physicochemical properties, stabilities, and antioxidant activities of anthocyanins from perilla leaves were investigated. The results showed that ultrasonic-assisted aqueous two-phase extraction yielded the highest content of anthocyanins, with the best color quality, stability, and antioxidant activities.
SUSTAINABLE CHEMISTRY AND PHARMACY
(2022)
Article
Horticulture
Rosanna Dimita, Samar Min Allah, Andrea Luvisi, Davide Greco, Luigi De Bellis, Rita Accogli, Carlo Mininni, Carmine Negro
Summary: This study found differences in volatile organic compounds (VOCs) emitted from two varieties of Perilla leaves at the microgreens stage, with significantly reduced emission after development. The results also indicated that the unique smell of microgreens Perilla leaves is variety-specific and not related to the amount of total phenols or antioxidant capacity of the leaves.
Article
Horticulture
Hideo Yoshida, Kanae Shimada, Shoko Hikosaka, Eiji Goto
Summary: This study investigated the effect of different UV-B irradiations on the accumulation of rosmarinic acid (RA) and anthocyanin, and the expression of genes related to biosynthetic pathways in red perilla. The results showed that UV-B irradiation at 6 W m(-2) for 16 h enhanced the concentration and content of RA in red perilla.
Review
Biochemistry & Molecular Biology
Gigi Adam, Silvia Robu, Mihaela-Magdalena Flutur, Oana Cioanca, Ingrid-Andrada Vasilache, Ana-Maria Adam, Cornelia Mircea, Aurel Nechita, Valeriu Harabor, AnaMaria Harabor, Monica Hancianu
Summary: This review aims to provide an updated understanding of the clinical applications of Perilla frutescens through human studies and outline potential directions for further research. The findings suggest significant clinical effects of Perilla frutescens extracts on allergic rhinoconjuctivitis, especially in young populations, as well as a potent hypolipemiant effect and potential improvements in cognitive function. Supplementary research in controlled clinical settings is needed to confirm these therapeutic effects.
Article
Chemistry, Applied
Tingting Zhang, Hualin Wang, Dongxiu Qi, Li Xia, Linlin Li, Xingjiang Li, Shaotong Jiang
Summary: A cellulose acetate membrane incorporated with Perilla frutescens anthocyanins and chamomile essential oil has been developed for food freshness monitoring and shelf-life extending. The membrane exhibits high sensitivity and reversibility towards pH stimuli, as well as good antibacterial and antioxidant activity.
CARBOHYDRATE POLYMERS
(2022)
Article
Agricultural Engineering
SuYun Ding, ZhiQian Yan, HuiPing Liu, Pei Chen, ShuYuan Shi, MengLi Chang
Summary: The polysaccharide PFP extracted from Perilla frutescens var. frutescens has antitumor activity by inhibiting tumor proliferation through blocking the cell cycle S phase, and also exhibits protective effects on mouse liver and thymus tissues.
INDUSTRIAL CROPS AND PRODUCTS
(2022)
Article
Plant Sciences
Payungsak Tantipaiboonwong, Komsak Pintha, Wittaya Chaiwangyen, Maitree Suttajit, Chakkrit Khanaree, Orawan Khantamat
Summary: Perilla leaves contain polyphenols with various biological effects. Fresh (PLEf) and dry (PLEd) Thai perilla leaf extracts were compared for their bioefficacies and bioactivities. PLEf and PLEd were rich in rosmarinic acid and bioactive phenolic compounds, but PLEd exhibited greater free radical scavenging activity. Both extracts suppressed intracellular ROS generation and exhibited antimutagenic and anti-inflammatory activities. PLEf showed higher cellular ROS suppression and higher antimutagenic and anti-inflammatory activities, which can be attributed to its combination of phytochemical components. Overall, PLEf and PLEd have the potential to serve as natural bioactive agents for potential health benefits.
Article
Chemistry, Analytical
Huairong Zhang, Yong Liu, Mei Yao, Wenxiu Han, Shusheng Zhang
Summary: Cathodic electrochemiluminesence (ECL) microscopy based on luminol analog L012 utilizes multiwalled carbon nanotubes (MWCNTs) as coreactant accelerators to convert oxygen and H2O2 into reactive oxygen species. The produced ROS oxidizes L012 to emit bright cathodic ECL illumination, enabling the imaging of nanotubes and nucleolin on tumor cells. By modifying MWCNTs with AS1411 aptamers, label-free ECL imaging of nucleolin on plasma membranes was achieved, providing a universal approach in single nanomaterial and single-cell imaging.
ANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Analytical
Lucia Simona Ferraraccio, Donatella Di Lisa, Laura Pastorino, Paolo Bertoncello
Summary: A simple procedure for incorporating enzymes into alginate hydrogels was reported, and electrochemiluminescence was used for detecting the enzymatic reactions. The immobilized enzymes showed maintained activity and good stability within the alginate hydrogels for up to 28 days. The sensitivity and selectivity of the encapsulated enzymes were demonstrated through testing real samples.
ANALYTICAL CHEMISTRY
(2022)
Article
Agricultural Engineering
Jin Hwan Lee, Yong-Sung Cho
Summary: The research aimed to compare phenolic contents and beneficial effects from different organs of Korean perilla species, showing significant variations in antioxidant and enzyme inhibitory activities. Perilla seeds, especially from P. frutescens and P. citriodora, exhibited predominant abilities, making them potential sources for natural nutraceutical uses.
INDUSTRIAL CROPS AND PRODUCTS
(2021)
Article
Food Science & Technology
Lihua Gu, Yue Jiang, Yuanyuan Han, Li Yang, Zhengtao Wang
Summary: A practical TLC-direct bioassay method was established for screening and evaluating antioxidant activity. The method involved a series of reactions, including the production of lipid peroxides by UV irradiation of linoleic acid and the conversion of peroxides into malondialdehyde. A dot blot test using TLC separation showed that substances with anti-lipid peroxidation activity appeared as white spots/zones on a red background. The method was validated for selectivity, sensitivity, linearity, precision, and recovery, and was successfully used to identify active antioxidant constituents from Perilla fruits.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Wen-Fang Tang, Hui-Ping Tsai, Yu-Hsiu Chang, Tein-Yao Chang, Chung-Fan Hsieh, Chia-Yi Lin, Guan-Hua Lin, Yu-Li Chen, Jia-Rong Jheng, Ping-Cheng Liu, Chuen-Mi Yang, Yuan-Fan Chin, Cheng Cheung Chen, Jyh-Hwa Kau, Yi-Jen Hung, Po-Shiuan Hsieh, Jim-Tong Horng
Summary: The study found that Perilla leaf extract could inhibit SARS-CoV-2 replication by inactivating the virus, blocking viral infection steps, reducing viral replication and inflammation response. Moreover, it showed additive-to-synergistic efficacy when used in combination with remdesivir.
BIOMEDICAL JOURNAL
(2021)
Article
Biochemistry & Molecular Biology
Zhan Li, Ying Wang, Lili Yu, Yongzhe Gu, Lijuan Zhang, Jun Wang, Lijuan Qiu
Summary: The increasing problem of soil salinization has a severe impact on soybean growth and development, leading to reduced yields and posing a serious threat to global crop production. In this study, the PfFAD3a gene was isolated from perilla and overexpressed in soybeans to assess the salt tolerance of transgenic plants. The results showed that overexpression of PfFAD3a increased the expression of PfFAD3a and the content of ff-linolenic acid in transgenic soybean plants, significantly enhancing their salt tolerance. Physiological and biochemical analysis revealed that overexpression of PfFAD3a increased the chlorophyll content and maximum photochemical efficiency of transgenic soybean plants under salt stress, while reducing the accumulation of MDA, H2O2, and O-circle(2), and increasing the activities of SOD, POD, CAT, and APX, as well as the production of proline and soluble sugar. In conclusion, the overexpression of PfFAD3a enhances salt tolerance in transgenic soybean plants through improved membrane fluidity and increased antioxidant capacity induced by C18:3.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
