期刊
MEDICAL MICROBIOLOGY AND IMMUNOLOGY
卷 198, 期 2, 页码 113-121出版社
SPRINGER
DOI: 10.1007/s00430-009-0111-z
关键词
Pseudomonas aeruginosa; Quorum sensing system; Homoserine lactone; Mast cells; Apoptosis; Calcium; Cytokines
资金
- National Natural Science Foundation of China [30571647]
- China National 973 project [2007CB512900]
Quorum sensing system is a cell-to-cell communication system that plays a pivotal role in virulence expression in bacteria. Recent advances have demonstrated that the Pseudomonas aeruginosa quorum sensing molecule, N-3-oxododecanoyl homoserine lactone (3OC(12)-HSL), exerts effects on mammalian cells and modulates host immune response. Mast cells (MCs) are strategically located in the tissues that are constantly exposed to external stimulus. Therefore, it is very much possible that 3OC(12)-HSL may interact with MCs. Little is known, however, about specific effects of 3OC(12)-HSL on MCs. To address this, we investigated the influence of 3OC(12)-HSL on cell viability, apoptosis, intracellular calcium and cytokine release in MCs. We found that at high concentrations (100 mu M), 3OC(12)-HSL inhibited proliferation and induced apoptosis in P815. The 3OC(12)-HSL treatment significantly increased intracellular calcium release in both P815 and HMC-1. We also observed that 3OC(12)-HSL-induced histamine release and degranulation in HMC-1 cells. Furthermore, 3OC(12)-HSL-induced IL-6 production at lower concentrations (6.25-12.5 mu M) but steadily reduced IL-6 production at high concentration (50-100 mu M). These data demonstrate that P. aeruginosa 3OC(12)-HSL affects MCs function.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据