期刊
MEASUREMENT SCIENCE AND TECHNOLOGY
卷 25, 期 2, 页码 -出版社
IOP PUBLISHING LTD
DOI: 10.1088/0957-0233/25/2/025701
关键词
glucose sensor; portable system; fluorescence
资金
- National Institutes of Health, National Institutes of Child Health and Human Development [R41HD069207]
- GE Healthcare
Glucose in non-invasively collected biofluids is generally in the micromolar range and thus, requires sensing methodologies capable of measuring glucose at these levels. Here, we present a small fluorometer system that can quantify glucose in the range of 0-5 mu M with resolution of similar to 0.07 mu M. It relies on the glucose binding protein (GBP) fluorescently labeled with two fluorophores. Fluorescence signals from the dual-labeled GBP are utilized in a ratiometric mode, making the measurements insensitive to variations in protein concentration and other systematic errors. Fluorescence is quantified by a miniature, dedicated ratiometric fluorometer that is powered via USB. Concentration is calculated using an ultra-mobile personal computer. The whole system is designed to be pocket sized suitable for point-of-care or bedside applications. Test results suggest that the system is a promising tool for accurate measurements of low glucose concentrations (0.1-10 mu M) in biological samples.
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